General information for g3749.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Phospholipase B1, membrane-associated.
Parent gene
- The parent gene g3749 is located on chr_3: 27807849..27809943 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3749 | g3749.t1 | isoform | g3749.t1 | 27807849 | 27809943 |
| chr_3 | g3749 | g3749.t1 | exon | g3749.t1.exon1 | 27807849 | 27808335 |
| chr_3 | g3749 | g3749.t1 | cds | g3749.t1.CDS1 | 27807849 | 27808335 |
| chr_3 | g3749 | g3749.t1 | exon | g3749.t1.exon2 | 27808803 | 27808917 |
| chr_3 | g3749 | g3749.t1 | cds | g3749.t1.CDS2 | 27808803 | 27808917 |
| chr_3 | g3749 | g3749.t1 | exon | g3749.t1.exon3 | 27808991 | 27809037 |
| chr_3 | g3749 | g3749.t1 | cds | g3749.t1.CDS3 | 27808991 | 27809037 |
| chr_3 | g3749 | g3749.t1 | exon | g3749.t1.exon4 | 27809102 | 27809314 |
| chr_3 | g3749 | g3749.t1 | cds | g3749.t1.CDS4 | 27809102 | 27809314 |
| chr_3 | g3749 | g3749.t1 | exon | g3749.t1.exon5 | 27809372 | 27809471 |
| chr_3 | g3749 | g3749.t1 | cds | g3749.t1.CDS5 | 27809372 | 27809471 |
| chr_3 | g3749 | g3749.t1 | exon | g3749.t1.exon6 | 27809598 | 27809943 |
| chr_3 | g3749 | g3749.t1 | cds | g3749.t1.CDS6 | 27809598 | 27809943 |
| chr_3 | g3749 | g3749.t1 | TSS | g3749.t1 | NA | NA |
| chr_3 | g3749 | g3749.t1 | TTS | g3749.t1 | NA | NA |
Sequences
>g3749.t1 Gene=g3749 Length=1308
ATGAACTCATTGAAATCATTCATTTTTCTCTGCATTGTTGCAGTCGCTAATCTACAGAGT
ACAAAAATCAGTCGAAACAGTGGCAATTTACTAACAAATTTGACACAAGTTCAAGAGTTC
AAAGACCACATTGTGACGAGCAGTTTGGACAATTCGTTAATGATTCGAATGTTTTATCGT
GGTTTTCGAGAGTTGTATAGTAATTTAATGAGTGGCACAGGTAATCGGCATAACAGTGAA
CGCTATCAGACAGCCATCTCAAATCGCGTTCCTTTCCCGTGCGTCGACGTCAAAAACTTT
CGCAGCAAGAAGAAACCAAAAAGTGTTCATCAATTGAGACCCGGAGATTTTGATATAGTT
GCTGCTATGGGAGATTCTTTGACTTCAGCGACTGCAGCTAATTCAGCTGCTTTACATGAA
TTACTTGTGGAAAATCGTGGATTAAGCTGGAGCATCGGTGGCCAAGGCAGTTGGCGCTCA
CATTTAACGCTTCCCAATATTCTTAAAGTTTTCAATCCAAATTTATATGGCTTTTCACTC
TCTGATGCTTACAATGTGCATAAAAGTGCTCAATTTAATATAGCTGAAAACATTGCAACA
ACAACTGATATGCCTTTCAATGCGAAAATCCTAATGCAACGAATGATGAAAGATCCAAGA
GTGGATATGAAGAAACATTGGAAATTGCTGACGTTAATGATTGGCGGAAATGATTTTTGT
TCAGACGTTTGTCATCAAAAAAATGCAACACAATGGCTAAATGAAGTTCAACGAAAATAT
CTTGTACAAACTTTACGTTATTTGCGAGATGCTTCTCCCAGAACACTTGTAAATTTAGTA
CCATCGCCTTTAGTAAATCTTCTTTTCTCTGTTGATCGAGTTGCGATTCCACTGTCGTGT
TATTTATCGATGCCAATTGAATGTAGTTGTTTATTTGGTAAAAAATATTCAAGAAACAGA
GAGCAATATCGTGTACTTGAAAGAAAGTTTACTCAAATTATGGAAGAAGTTAGTTTCATG
TCAGAGTTACATACTAATGATTTCACTGTCGTTTATCAGCCATTTTTCAAAGATGCAAGC
ATTTTCTATCAAAATAATGATAAACCAGATCTCACAATTATGGCAATTGATTGTTTACAT
TTGAGTCAAAAAGGACATGCATTATCAGCCAATGGAATCTGGAATAACATGCTTGAACCA
GTTGGAAGAAAATCATTAGGATTGCAACCATTATTCAAAAAATTCAATTGCCCAACAGAA
CGTCATCCTTATATTTTCACAAATTATAATAGCAACAATTTCTTTTAA
>g3749.t1 Gene=g3749 Length=435
MNSLKSFIFLCIVAVANLQSTKISRNSGNLLTNLTQVQEFKDHIVTSSLDNSLMIRMFYR
GFRELYSNLMSGTGNRHNSERYQTAISNRVPFPCVDVKNFRSKKKPKSVHQLRPGDFDIV
AAMGDSLTSATAANSAALHELLVENRGLSWSIGGQGSWRSHLTLPNILKVFNPNLYGFSL
SDAYNVHKSAQFNIAENIATTTDMPFNAKILMQRMMKDPRVDMKKHWKLLTLMIGGNDFC
SDVCHQKNATQWLNEVQRKYLVQTLRYLRDASPRTLVNLVPSPLVNLLFSVDRVAIPLSC
YLSMPIECSCLFGKKYSRNREQYRVLERKFTQIMEEVSFMSELHTNDFTVVYQPFFKDAS
IFYQNNDKPDLTIMAIDCLHLSQKGHALSANGIWNNMLEPVGRKSLGLQPLFKKFNCPTE
RHPYIFTNYNSNNFF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g3749.t1 | CDD | cd01824 | Phospholipase_B_like | 108 | 402 | 6.0659E-121 |
| 6 | g3749.t1 | Gene3D | G3DSA:3.40.50.1110 | - | 96 | 395 | 3.5E-8 |
| 2 | g3749.t1 | PANTHER | PTHR21325 | PHOSPHOLIPASE B, PLB1 | 75 | 434 | 5.8E-110 |
| 3 | g3749.t1 | PANTHER | PTHR21325:SF31 | PHOSPHOLIPASE B1, MEMBRANE-ASSOCIATED | 75 | 434 | 5.8E-110 |
| 1 | g3749.t1 | Pfam | PF00657 | GDSL-like Lipase/Acylhydrolase | 120 | 391 | 7.4E-10 |
| 8 | g3749.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 9 | g3749.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 6 | - |
| 10 | g3749.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 7 | 15 | - |
| 11 | g3749.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 20 | - |
| 7 | g3749.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 435 | - |
| 4 | g3749.t1 | SUPERFAMILY | SSF52266 | SGNH hydrolase | 181 | 401 | 1.39E-9 |
| 5 | g3749.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004620 | phospholipase activity | MF |
| GO:0016788 | hydrolase activity, acting on ester bonds | MF |
KEGG
Orthology
- This transcript has been annotated with K14621 (phospholipase B1, membrane-associated)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
