General information for g3883.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Dual specificity mitogen-activated protein kinase kinase 4.
Parent gene
- The parent gene g3883 is located on chr_3: 28611835..28612745 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3883 | g3883.t3 | TSS | g3883.t3 | 28611026 | 28611026 |
| chr_3 | g3883 | g3883.t3 | isoform | g3883.t3 | 28611269 | 28612427 |
| chr_3 | g3883 | g3883.t3 | exon | g3883.t3.exon1 | 28611269 | 28611357 |
| chr_3 | g3883 | g3883.t3 | cds | g3883.t3.CDS1 | 28611269 | 28611357 |
| chr_3 | g3883 | g3883.t3 | exon | g3883.t3.exon2 | 28611485 | 28611564 |
| chr_3 | g3883 | g3883.t3 | cds | g3883.t3.CDS2 | 28611485 | 28611564 |
| chr_3 | g3883 | g3883.t3 | exon | g3883.t3.exon3 | 28611622 | 28611767 |
| chr_3 | g3883 | g3883.t3 | cds | g3883.t3.CDS3 | 28611622 | 28611767 |
| chr_3 | g3883 | g3883.t3 | exon | g3883.t3.exon4 | 28611824 | 28612427 |
| chr_3 | g3883 | g3883.t3 | cds | g3883.t3.CDS4 | 28611824 | 28612426 |
| chr_3 | g3883 | g3883.t3 | TTS | g3883.t3 | 28613023 | 28613023 |
Sequences
>g3883.t3 Gene=g3883 Length=919
ATGGATAACGACAGTAAAAAAGCACTTAAATTAGTTTTCCCGAGTCAATCAAGAACAGCC
CATAGGCCCCATCAATCGCTACCTACAAATCGTGATCGATTTCGTAATGCTCATTCGGAT
TCTGGAAAATTGCAATTTAGCAGTACTGAGATATATGATTTTACAGCAGACGATCTCTTA
GACCTTGGTGAAATCGGAAGAGGCGGTTTTGGAAGTGTTAATAAAATGATTTTTAAGAAA
ACAGAAAAAGTAATGGCAGTTAAAAGAATACGATGTTCTAATGTTGTTGATGAGAAGGAG
CAAAAAAGAATTATGGATCTGGATGTAGCAATGAAATCGTCTAATGATTGCAAATATATT
GTACAATTTTATGGAGCTATTTTTAAAGAAGGAGATTGTTGGATTTGTATGGAAATTCTT
TCTGTTTCCCTTGATAAATTCTATAAGTATGTCTATGAGAGACAAAATCAACGCATACCT
GAAAACATTCTTGGACAAATAACCGTTGCAACAGTAAACGCGTTAAATTATTTAAAAGAA
AAATTAAAAATCATCCATAGAGATGTTAAACCGAGCAACATTCTGCTTGCTGAGCGTGGT
GATATCAAGCTCTGTGATTTCGGTATTTCCGGACAATTGATTGATTCAATTGCAAGAACT
AAAGACGCTGGTTGTCGCCCCTATATGGCACCTGAGAGAATCGATCCAGAATTTTCTCAT
AAAGGCTATGATGTTCGATCTGATGTCTGGTCACTTGGAATTACTTTAATGGAAGTTGCG
ACTGGAAAATTTCCTTATCCTAGCAAATGGGCCTCAGTTTTTGAGCAATTATATCAAGTT
GTGCAAGGCCCACCTCCACAATTAACTCGCCAATATAATGGATTTGAATTTACTGAAGAA
TTTTGTAATTTTGTTAATA
>g3883.t3 Gene=g3883 Length=306
MDNDSKKALKLVFPSQSRTAHRPHQSLPTNRDRFRNAHSDSGKLQFSSTEIYDFTADDLL
DLGEIGRGGFGSVNKMIFKKTEKVMAVKRIRCSNVVDEKEQKRIMDLDVAMKSSNDCKYI
VQFYGAIFKEGDCWICMEILSVSLDKFYKYVYERQNQRIPENILGQITVATVNALNYLKE
KLKIIHRDVKPSNILLAERGDIKLCDFGISGQLIDSIARTKDAGCRPYMAPERIDPEFSH
KGYDVRSDVWSLGITLMEVATGKFPYPSKWASVFEQLYQVVQGPPPQLTRQYNGFEFTEE
FCNFVN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g3883.t3 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 37 | 140 | 7.7E-34 |
| 6 | g3883.t3 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 141 | 306 | 3.5E-46 |
| 11 | g3883.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 41 | - |
| 10 | g3883.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 25 | 39 | - |
| 2 | g3883.t3 | PANTHER | PTHR48013 | DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED | 21 | 306 | 1.5E-143 |
| 3 | g3883.t3 | PANTHER | PTHR48013:SF15 | DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4 | 21 | 306 | 1.5E-143 |
| 1 | g3883.t3 | Pfam | PF00069 | Protein kinase domain | 62 | 276 | 2.6E-48 |
| 8 | g3883.t3 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 65 | 88 | - |
| 7 | g3883.t3 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 184 | 196 | - |
| 12 | g3883.t3 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 59 | 306 | 36.541 |
| 9 | g3883.t3 | SMART | SM00220 | serkin_6 | 59 | 298 | 2.4E-41 |
| 4 | g3883.t3 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 46 | 283 | 4.05E-57 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
