Gene loci information

Transcript annotation

  • This transcript has been annotated as Probable enoyl-CoA hydratase, mitochondrial.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g3891 g3891.t3 TTS g3891.t3 28634277 28634277
chr_3 g3891 g3891.t3 isoform g3891.t3 28634381 28635396
chr_3 g3891 g3891.t3 exon g3891.t3.exon1 28634381 28634847
chr_3 g3891 g3891.t3 cds g3891.t3.CDS1 28634381 28634847
chr_3 g3891 g3891.t3 exon g3891.t3.exon2 28634912 28635396
chr_3 g3891 g3891.t3 cds g3891.t3.CDS2 28634912 28635122
chr_3 g3891 g3891.t3 TSS g3891.t3 28635461 28635461

Sequences

>g3891.t3 Gene=g3891 Length=952
ATGGCTTCAATCACAAAGATCGTATCATCATTATCACGTGCACCTGCATTAAAGCAACAA
TACAGACTTTTCTGTGCGGCAGGTAAAATAAATGATTAAATTTAGAAAACAATGTGCCGA
CATATTCATTTTTTGATTTCATGAACTTTGATCGTGAAAATTTGCAGCAAACAAATATGA
ATTTATTGTAAAAGATGTTGCTGGCGAGAAAAAGAATGTTGGTTTGATTACATTGAATCG
ACCAAAAGCTCTTAATGCTTTAAATAAAGGTTTAATGACAGAAGTTGCCAGAGCATTGAA
TGAATTTGATTCTGATAAATCTATTGGTGCAATTGTCATAACTGGAAATGAGAAAGCTTT
TGCTGCCGGTGCCGATATCAAAGAAATGCAGGGTAACACATATGCCGATTGCATTCTTAA
TAACTTTTTAGCTGATTGGACAAAAGTTGCAAGCACAAAAAAACCAGTCATTGCAGCAGT
TAATGGATATGCTCTTGGAGGCGGTTGTGAATTAGCAATGATGTGCGATATAATTTATGC
TGGAGATAAAGCTCGTTTCGGTCAACCTGAGATCAATATTGGAACAATTCCCGGAGCTGG
CGGTACACAACGTTTAACACGCGTGATTGGAAAATCAAAGGCAATGGAAATGTGTTTGAC
AGGAAACATGATTACAGCTGAAGAAGCTGAAAAGTCAGGTTTAGTAAGCAAAGTGTTTCC
AGCTGATCAGTTAGTCAAAGAGGCAGTTAAACTCGGTGAAAAAATTAGTGCTCAATCGCC
ATTGACAGTTGCTCTTGCAAAAGAAGCCGTTAATGTTGCATATGAGAGCACACTTGCTGA
AGGTTTGAGATTTGAGAGAAGATACTTCCACGCAACATTCTCAACCAAAGATCAGAAAGA
AGGAATGTTGGCATTTATTGAGAAACGTGCACCTAACTTTACCAGCGAATAA

>g3891.t3 Gene=g3891 Length=225
MTEVARALNEFDSDKSIGAIVITGNEKAFAAGADIKEMQGNTYADCILNNFLADWTKVAS
TKKPVIAAVNGYALGGGCELAMMCDIIYAGDKARFGQPEINIGTIPGAGGTQRLTRVIGK
SKAMEMCLTGNMITAEEAEKSGLVSKVFPADQLVKEAVKLGEKISAQSPLTVALAKEAVN
VAYESTLAEGLRFERRYFHATFSTKDQKEGMLAFIEKRAPNFTSE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
8 g3891.t3 CDD cd06558 crotonase-like 1 164 9.78696E-66
7 g3891.t3 Gene3D G3DSA:3.90.226.10 - 1 167 1.9E-67
6 g3891.t3 Gene3D G3DSA:1.10.12.10 - 168 224 7.8E-27
2 g3891.t3 PANTHER PTHR11941:SF54 ENOYL-COA HYDRATASE, MITOCHONDRIAL 1 220 9.2E-83
3 g3891.t3 PANTHER PTHR11941 ENOYL-COA HYDRATASE-RELATED 1 220 9.2E-83
1 g3891.t3 Pfam PF00378 Enoyl-CoA hydratase/isomerase 1 224 1.7E-82
5 g3891.t3 ProSitePatterns PS00166 Enoyl-CoA hydratase/isomerase signature. 66 86 -
4 g3891.t3 SUPERFAMILY SSF52096 ClpP/crotonase 2 224 1.66E-78

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0003824 catalytic activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values