Gene loci information

Transcript annotation

  • This transcript has been annotated as Cytochrome P450 18a1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g4003 g4003.t2 TSS g4003.t2 29462923 29462923
chr_3 g4003 g4003.t2 isoform g4003.t2 29463946 29464913
chr_3 g4003 g4003.t2 exon g4003.t2.exon1 29463946 29464136
chr_3 g4003 g4003.t2 exon g4003.t2.exon2 29464197 29464741
chr_3 g4003 g4003.t2 cds g4003.t2.CDS1 29464327 29464741
chr_3 g4003 g4003.t2 exon g4003.t2.exon3 29464819 29464913
chr_3 g4003 g4003.t2 cds g4003.t2.CDS2 29464819 29464913
chr_3 g4003 g4003.t2 TTS g4003.t2 NA NA

Sequences

>g4003.t2 Gene=g4003 Length=831
GTGTGATAAATGCAGAAGGTGCACTTTGGAAGTCAGAAAGGCGCTTCTTGCATGAGAAAC
TTAGACGTTTTGGAATGACGCATTTAAATAGTAAAAACAACAAATTGCACGACATCATAA
ATGTAAGTTTTTTCCTTCTTTTTTTTCGGTGCCCTTGACAAAACTAAAAAATTCACACAA
CTATAGTCTTTATGTACACGTGCATGCATTTAGAATGAAATCAATGAGCTTCTAACGACG
TTAAGGCACGAAACGAAGAAGCCAATCGACTTGGAACCGCACTTTGCCGTCTCAATTAGC
AACGTCGTGTGCAATTTATTAATGTCTGTTCGTTTTACACGAAATGATCCAAAATTCATC
AGATTTAATAGCATGATTGAGGAGGGCATGAAATTATTTGGAAAAATCACTTCAGTCGAT
TACATTCCAATTACTCAGTATTTTCCATCATTCCAAACAGCAAAGAGTCAAATCGCTAGC
AATCGCGAAGAAATGTTTGAATTTTATCGCGAAGTGATTGAAGACCATCGAAAGACATTT
GATCGTAATAACATTCGCGATTTAGTGGACTTTTATCTATTAGAAATTGAACTTGCAAAG
GAAAATGGAACTGAACATGAGCTTTTTAATGGTCGAAATCATGATGAACAAATAATGCAA
GTGATGGGAGATTTATTCAGTGCTGGTATGGAAACCATCAAGACGACATTGCTTTGGTTG
ATCGTCTTTATGTTGCGACTTACAATATCTGCCAAATACCGAGAGTACAATTTACGAAGT
GCTTCGAATAAGCTCGATAGTGCCACTAGCCACAACACACTCACCGATGAT

>g4003.t2 Gene=g4003 Length=170
MSVRFTRNDPKFIRFNSMIEEGMKLFGKITSVDYIPITQYFPSFQTAKSQIASNREEMFE
FYREVIEDHRKTFDRNNIRDLVDFYLLEIELAKENGTEHELFNGRNHDEQIMQVMGDLFS
AGMETIKTTLLWLIVFMLRLTISAKYREYNLRSASNKLDSATSHNTLTDD

Protein features from InterProScan

Transcript Database ID Name Start End E.value
5 g4003.t2 Gene3D G3DSA:1.10.630.10 Cytochrome p450 1 142 0
2 g4003.t2 PANTHER PTHR24300 CYTOCHROME P450 508A4-RELATED 1 139 0
3 g4003.t2 PANTHER PTHR24300:SF334 CYTOCHROME P450 18A1 1 139 0
1 g4003.t2 Pfam PF00067 Cytochrome P450 2 139 0
4 g4003.t2 SUPERFAMILY SSF48264 Cytochrome P450 2 139 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed