Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Sterol O-acyltransferase 1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g4101 g4101.t2 TTS g4101.t2 30581898 30581898
chr_3 g4101 g4101.t2 isoform g4101.t2 30581961 30582484
chr_3 g4101 g4101.t2 exon g4101.t2.exon1 30581961 30582484
chr_3 g4101 g4101.t2 cds g4101.t2.CDS1 30581961 30582473
chr_3 g4101 g4101.t2 TSS g4101.t2 NA NA

Sequences

>g4101.t2 Gene=g4101 Length=524
TTCATTCATGGATGAATGCATTTGCAGAGTTCCTTAGATTTTCTGATCGACAATTTTATC
GCGATTGGTGGAATAGTACAAATTTTTCAGTTTATTATCGTACATGGAATGTAATTGTGC
ATGATTGGCTTTATCAGTATGTCTACAGAGACCTTTATTTGCACATTTTCAAAAAGAAGC
GTGGAATTTGCCAGTTTTTAGTATTCTGCTTATCAGCAATCTTTCATGAATACATTCTTG
GTTTTACCTTCAGATTTTGTTATCCAATTTTATTCGTAGAATTTCAAGGTGCTGGTGTAT
TGTTGTTCTTTGCGACAAGAAAAGATATGAAAACAATTGGAAATTGCTTCATGTGGATGA
GTATTGCTATAGGAATGGGAATATTGCTAAGCTTATATCACATGGAATATTATGCACGCC
AGAATTGCTCATGGGACAAAGATAAAATTGTCAACTATTTTATTCCTATAAGTTGGAGTT
GTAATGGATTAAAAATCAGTGAAAATTGGAAGATTCAATTTTAA

>g4101.t2 Gene=g4101 Length=170
MNAFAEFLRFSDRQFYRDWWNSTNFSVYYRTWNVIVHDWLYQYVYRDLYLHIFKKKRGIC
QFLVFCLSAIFHEYILGFTFRFCYPILFVEFQGAGVLLFFATRKDMKTIGNCFMWMSIAI
GMGILLSLYHMEYYARQNCSWDKDKIVNYFIPISWSCNGLKISENWKIQF

Protein features from InterProScan

Transcript Database ID Name Start End E.value
2 g4101.t2 PANTHER PTHR10408:SF8 O-ACYLTRANSFERASE 1 150 3.8E-59
3 g4101.t2 PANTHER PTHR10408 STEROL O-ACYLTRANSFERASE 1 150 3.8E-59
1 g4101.t2 Pfam PF03062 MBOAT, membrane-bound O-acyltransferase family 6 129 5.0E-24
8 g4101.t2 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 57 -
13 g4101.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 58 76 -
9 g4101.t2 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 77 81 -
11 g4101.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 82 101 -
7 g4101.t2 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 102 112 -
12 g4101.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 113 131 -
10 g4101.t2 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 132 170 -
6 g4101.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 59 76 -
4 g4101.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 80 102 -
5 g4101.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 109 131 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0008374 O-acyltransferase activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed