General information for g4115.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Wee1-like protein kinase.
Parent gene
- The parent gene g4115 is located on chr_3: 30720136..30723022 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g4115 | g4115.t3 | TSS | g4115.t3 | 30718307 | 30718307 |
| chr_3 | g4115 | g4115.t3 | isoform | g4115.t3 | 30718385 | 30719621 |
| chr_3 | g4115 | g4115.t3 | exon | g4115.t3.exon1 | 30718385 | 30718551 |
| chr_3 | g4115 | g4115.t3 | cds | g4115.t3.CDS1 | 30718385 | 30718551 |
| chr_3 | g4115 | g4115.t3 | exon | g4115.t3.exon2 | 30718617 | 30718837 |
| chr_3 | g4115 | g4115.t3 | cds | g4115.t3.CDS2 | 30718617 | 30718837 |
| chr_3 | g4115 | g4115.t3 | exon | g4115.t3.exon3 | 30718904 | 30719621 |
| chr_3 | g4115 | g4115.t3 | cds | g4115.t3.CDS3 | 30718904 | 30719619 |
| chr_3 | g4115 | g4115.t3 | TTS | g4115.t3 | NA | NA |
Sequences
>g4115.t3 Gene=g4115 Length=1106
ATGGATACAGAAAGTAGCGATTTTGATTCTCCGGATATGAATTCAAGTTTGGAACGACAG
TCATCTCCAGAGAGAATGGTTCAGCCGCGAAAATTAAAATTTTCTCCAAAGAGTCCTCAT
AAACTTCAACAATTTTCACCGGATATAAACAGAATTAAGGCATTACGTCTTTTTGATTCT
CCAAGAACGCCTAAATCTCTAATGTCAACAAAATTTCAAAAATACATGTCTACAAATAAT
GAACAGCAACATGATGAAAATAATTATGAAGTGATTGAACGACCAAAGCCTGAAATTCCT
CCTTTTATGCATATAAATGTCAACAACAATAATCATATTTCAAAACCAAAGCCGTTTCCA
CTTTATAAACAATTACATGAAAACTTATCTGCAAATATCAATCCATTTACACCGCCAGGA
ATATCATTACGCAAGAAAAGAGCTCGATTTGAAGGAGATTTTCTTAATTCTACTATGCCT
ATTATCAGTAACAATTCGATAAAACCAAATAATTTAAATGCAAACAAATGGCTGCATCAT
TGTAATAATAATAAGGAACAAATAATTAATCCATTGAATGCAACGACTGCATGTGATTCT
TTTGAGCTGTGCTATGATGAGGAGCTAAATGAGTTTAGGCAAGCGCCAAAACGACTTGCC
TTACAAGAATCTAATATTACACGATATGAGAAGGAGTTCGTTGAATTGAAATTATTGGGT
GTTGGGGAATTTGGTTTAGTTTATCAGTGCCTTAATCGTTTAGATGGCTGTATTTATGCT
ATTAAAAGAAGCATTAAACCAGTCGCGGGTAGTTCGTTTGAAAAAATATCATTGAACGAA
GTTTATGCGCATGCTGTTCTAGGCAAGCATGATAATGTCGTTAGATATTATTCAGCATGG
GCCGAAAATAATCATATGTTAATTCAAAATGAGTATTGTAATGGAGGAAGCTTACAAAGT
CTCTTACAAAAGAGAAATCTAAATGAAGCTGAATTGAAAATACTCCTATTACAAATTGCC
GAAGGATTAAAATATATTCATTCACATAATCTCGTTCATATGGACATTAAAGCAGGAAAT
ATATTTATTGCAAAGCCACAAGTCAA
>g4115.t3 Gene=g4115 Length=368
MDTESSDFDSPDMNSSLERQSSPERMVQPRKLKFSPKSPHKLQQFSPDINRIKALRLFDS
PRTPKSLMSTKFQKYMSTNNEQQHDENNYEVIERPKPEIPPFMHINVNNNNHISKPKPFP
LYKQLHENLSANINPFTPPGISLRKKRARFEGDFLNSTMPIISNNSIKPNNLNANKWLHH
CNNNKEQIINPLNATTACDSFELCYDEELNEFRQAPKRLALQESNITRYEKEFVELKLLG
VGEFGLVYQCLNRLDGCIYAIKRSIKPVAGSSFEKISLNEVYAHAVLGKHDNVVRYYSAW
AENNHMLIQNEYCNGGSLQSLLQKRNLNEAELKILLLQIAEGLKYIHSHNLVHMDIKAGN
IFIAKPQV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g4115.t3 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 223 | 311 | 8.7E-28 |
| 6 | g4115.t3 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 312 | 368 | 1.8E-15 |
| 11 | g4115.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 41 | - |
| 10 | g4115.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 8 | 23 | - |
| 2 | g4115.t3 | PANTHER | PTHR11042 | EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED | 26 | 365 | 2.2E-73 |
| 3 | g4115.t3 | PANTHER | PTHR11042:SF75 | WEE1-LIKE PROTEIN KINASE 2 | 26 | 365 | 2.2E-73 |
| 1 | g4115.t3 | Pfam | PF00069 | Protein kinase domain | 234 | 365 | 5.5E-36 |
| 8 | g4115.t3 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 239 | 262 | - |
| 7 | g4115.t3 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 351 | 363 | - |
| 12 | g4115.t3 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 233 | 368 | 26.379 |
| 9 | g4115.t3 | SMART | SM00220 | serkin_6 | 233 | 368 | 1.9E-7 |
| 4 | g4115.t3 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 218 | 366 | 1.5E-39 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
