Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Probable cytochrome P450 313a1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g4192 g4192.t1 isoform g4192.t1 7326 8486
chr_2 g4192 g4192.t1 exon g4192.t1.exon1 7326 8175
chr_2 g4192 g4192.t1 cds g4192.t1.CDS1 7326 8175
chr_2 g4192 g4192.t1 exon g4192.t1.exon2 8314 8486
chr_2 g4192 g4192.t1 cds g4192.t1.CDS2 8314 8486
chr_2 g4192 g4192.t1 TSS g4192.t1 NA NA
chr_2 g4192 g4192.t1 TTS g4192.t1 NA NA

Sequences

>g4192.t1 Gene=g4192 Length=1023
ATGAAATGCTTGATGAAAAATTTAAGTGAAATGGAAGGAAAAGGAGAATTTAATGTTTTT
CACATCATGTTTGCATTATCACTTGATAATATTCTTTCTATAATGAATTTAGAAGCAAAT
ATTCAAGATTTAAATAATAAAGATAGACTGATCCATATTAAAAATATGAAAATTTGGGCT
CAAACAAAAATAATCAGAAGTCTTTTTTGGTGGCTATATCCTGACATAATCTTTAATTAT
ACAAAATTAGGAAAAAATCAAATAAAAGCCATGTCAGAAATTAATAAAAATTTTATTTCA
AAAATTATTGATCAAAACTATTTGGTTGAAAATTATGAAAAACCTAAAACATTTGTGCAA
GCATTAATTGACCCAAAGAATAAATTGACAAAAAATGAAATTTCTGACGAAATTTTTTGT
TTAATGATTGCAGCTCAAGATTCATCAGCAGTTACAGGATCAACTGTTCTTCTTATGTTG
GCAATGCATAGGAATATTCAAGAAAAGGTTGTTGATGAGCTTAAGGAAATAATTGGAAAA
TCAAAAGACTTTTATTTGGATATTGAACAATTGAATGAACTCAAATATCTTGAGATGGTC
ATTTGTGAAGTTCTAAGACTTTTCCCAGTTGCTCCATTTAATATTAGAAGTAATGACAAT
GAAATTATTTCTACAGAAGGATATATCATTCCAGTAAATTCAAATCTTTTTATAAATTTC
TTTGATGTCCATCACGACACATCAATTTGGGGATACGATGCTGATAAATTTAAACCAGAA
AGATTCGAGCAAGAAAGTTTTAGAAAAATTCATCCATATGCTTTTGTGCCATTCTCTAAA
GGCAAAAGAATGTGTCTCGGCTATCGATATGCGATTCTCTTTATAAAAATGCAAATTGCA
AATGTTCTTTTAAAATATGAAATTAATACAAGCTTGAAGTATGAAAATATAAATTACAAA
ATTGGTATCACACTAGTTATTCAACAGGGCTATAAAATAAGTTTAAAAAAGAGGAAAAAA
TAA

>g4192.t1 Gene=g4192 Length=340
MKCLMKNLSEMEGKGEFNVFHIMFALSLDNILSIMNLEANIQDLNNKDRLIHIKNMKIWA
QTKIIRSLFWWLYPDIIFNYTKLGKNQIKAMSEINKNFISKIIDQNYLVENYEKPKTFVQ
ALIDPKNKLTKNEISDEIFCLMIAAQDSSAVTGSTVLLMLAMHRNIQEKVVDELKEIIGK
SKDFYLDIEQLNELKYLEMVICEVLRLFPVAPFNIRSNDNEIISTEGYIIPVNSNLFINF
FDVHHDTSIWGYDADKFKPERFEQESFRKIHPYAFVPFSKGKRMCLGYRYAILFIKMQIA
NVLLKYEINTSLKYENINYKIGITLVIQQGYKISLKKRKK

Protein features from InterProScan

Transcript Database ID Name Start End E.value
14 g4192.t1 Gene3D G3DSA:1.10.630.10 Cytochrome p450 1 340 1.3E-69
2 g4192.t1 PANTHER PTHR24291:SF140 CYTOCHROME P450 4C3-RELATED 5 338 7.7E-66
3 g4192.t1 PANTHER PTHR24291 CYTOCHROME P450 FAMILY 4 5 338 7.7E-66
11 g4192.t1 PRINTS PR00385 P450 superfamily signature 144 161 1.2E-6
5 g4192.t1 PRINTS PR00463 E-class P450 group I signature 153 179 1.1E-12
7 g4192.t1 PRINTS PR00463 E-class P450 group I signature 198 216 1.1E-12
8 g4192.t1 PRINTS PR00385 P450 superfamily signature 199 210 1.2E-6
6 g4192.t1 PRINTS PR00463 E-class P450 group I signature 275 285 1.1E-12
9 g4192.t1 PRINTS PR00385 P450 superfamily signature 276 285 1.2E-6
4 g4192.t1 PRINTS PR00463 E-class P450 group I signature 285 308 1.1E-12
10 g4192.t1 PRINTS PR00385 P450 superfamily signature 285 296 1.2E-6
1 g4192.t1 Pfam PF00067 Cytochrome P450 1 311 3.8E-54
13 g4192.t1 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 278 287 -
12 g4192.t1 SUPERFAMILY SSF48264 Cytochrome P450 2 338 1.57E-69

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed