Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Cytochrome P450 6a22.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g43 g43.t1 TSS g43.t1 405320 405320
chr_3 g43 g43.t1 isoform g43.t1 405368 406249
chr_3 g43 g43.t1 exon g43.t1.exon1 405368 405712
chr_3 g43 g43.t1 cds g43.t1.CDS1 405368 405712
chr_3 g43 g43.t1 exon g43.t1.exon2 405771 405993
chr_3 g43 g43.t1 cds g43.t1.CDS2 405771 405993
chr_3 g43 g43.t1 exon g43.t1.exon3 406050 406249
chr_3 g43 g43.t1 cds g43.t1.CDS3 406050 406249
chr_3 g43 g43.t1 TTS g43.t1 406684 406684

Sequences

>g43.t1 Gene=g43 Length=768
ATGATTTTGACCTTGATTCTTGTTTCATTGCTCACAATTTTTGTTTACATAAAGTCTCGA
TACAATTTTTGGTCTTCTCATGGATTTCCAAGTGCAAAAGCAAAATTTTTAATAGGAAAT
CTTGATGGTGTTGGTCGTTCAAAAAATACTGCACAGAAATTAAAAGAAATTTATGATGAA
TTTCAAGAAAAAGAAGATTTTGTTGGAATTTATTTATTGATTAGTCCAGCAATATTAATT
CTCAAGCCAGAAATCATAAGACTCGTGTTGGTGAAAGATTTTTCAAATTTTAGTGATCGT
GGAATGTATTACAATAAAAAAGATGATCCATTAAGTGCTAATTTGTTGACAATGCGTGTA
GAAGAATGGAAACCAATGAGAACTAAAATCACTCCAACATTTAGTACAGCAAAAATAAAA
ACAATGTTTGATATCGTCTCGAATATAACTGATAATCTTGTTGACAAGTTATCAACAAGC
GATGTTTCAGGGACAAACATTACAGAGACAATTGCTGCCTATTCGACCGATGTTATTTCA
AATATCGCTTTTGGTATCAGTTCAAATTGTTTAGCCGATCCTAATTCGGAAATTAGAAAA
TACAGCAAATTAATTTTCGCAATGAGTCCACGAAGACTTATGAAATTTTTCATTTCATCA
CAATTTCCAAAATTTGCAGCAGCTCTTGGATTTACATCGACTAATAAAATTGCATCTGAT
TATTTTATGAAACTTTACGGAAGTATCCGCCAATTCCAATGTCATTGA

>g43.t1 Gene=g43 Length=255
MILTLILVSLLTIFVYIKSRYNFWSSHGFPSAKAKFLIGNLDGVGRSKNTAQKLKEIYDE
FQEKEDFVGIYLLISPAILILKPEIIRLVLVKDFSNFSDRGMYYNKKDDPLSANLLTMRV
EEWKPMRTKITPTFSTAKIKTMFDIVSNITDNLVDKLSTSDVSGTNITETIAAYSTDVIS
NIAFGISSNCLADPNSEIRKYSKLIFAMSPRRLMKFFISSQFPKFAAALGFTSTNKIASD
YFMKLYGSIRQFQCH

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g43.t1 Gene3D G3DSA:1.10.630.10 Cytochrome p450 12 253 7.0E-40
2 g43.t1 PANTHER PTHR24292 CYTOCHROME P450 3 245 5.4E-63
3 g43.t1 PANTHER PTHR24292:SF95 CYP6A16, ISOFORM B-RELATED 3 245 5.4E-63
5 g43.t1 PRINTS PR00464 Group II E-class P450 signature 120 140 2.2E-7
4 g43.t1 PRINTS PR00464 Group II E-class P450 signature 173 191 2.2E-7
1 g43.t1 Pfam PF00067 Cytochrome P450 34 214 1.9E-22
9 g43.t1 Phobius SIGNAL_PEPTIDE Signal peptide region 1 19 -
10 g43.t1 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 2 -
11 g43.t1 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 3 14 -
12 g43.t1 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 15 19 -
8 g43.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 20 255 -
6 g43.t1 SUPERFAMILY SSF48264 Cytochrome P450 20 251 1.44E-30

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0004497 monooxygenase activity MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values