General information for g4376.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as 6-pyruvoyl tetrahydrobiopterin synthase.
Parent gene
- The parent gene g4376 is located on chr_2: 1727256..1729115 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4376 | g4376.t1 | TSS | g4376.t1 | 1726623 | 1726623 |
| chr_2 | g4376 | g4376.t1 | isoform | g4376.t1 | 1726694 | 1727256 |
| chr_2 | g4376 | g4376.t1 | exon | g4376.t1.exon1 | 1726694 | 1726764 |
| chr_2 | g4376 | g4376.t1 | cds | g4376.t1.CDS1 | 1726694 | 1726764 |
| chr_2 | g4376 | g4376.t1 | exon | g4376.t1.exon2 | 1726821 | 1727256 |
| chr_2 | g4376 | g4376.t1 | cds | g4376.t1.CDS2 | 1726821 | 1727256 |
| chr_2 | g4376 | g4376.t1 | TTS | g4376.t1 | 1727515 | 1727515 |
Sequences
>g4376.t1 Gene=g4376 Length=507
ATGACAACATCACGCCCAATTGTTGATATCATTCGTAAAGAAAGTTTTTCTGCTTGTCAT
CGGCTCCACAGTCCATACCTGAGCGATGAAGAAAATCGAGAAGTTTTTGGAAAATGCAAT
AATGAGAATGGTCATGGTCACAATTATACAGTAGAAATAAAAATTAGAGGTCACGTTGAT
GCAAAAACTGGAATGGTTATGAATTTAGTTGATTTAAAGGCATTGATTGAAAAATGCATC
ATGAAACCATTGGATCATAAAAATCTCGATAAAGATGTGGAATATTTCAAAACAGTTCCT
TCTACTACAGAAAATTTAGCAGTCTTTATTTTTGACTCGTTGAAAAAAGCGTTACTAAAA
CCTGAACTTTTATATGAGGTCAAAATTTATGAAACTGATAAAAATTCTGTGATATACCGT
GGAAAAGTCAAGAATATTTATAGTAATGGAATGAGAAGAGTGTCAGCTAATATCTGTGCG
AACATGAGTAGTGACTCTGATTCGTAA
>g4376.t1 Gene=g4376 Length=168
MTTSRPIVDIIRKESFSACHRLHSPYLSDEENREVFGKCNNENGHGHNYTVEIKIRGHVD
AKTGMVMNLVDLKALIEKCIMKPLDHKNLDKDVEYFKTVPSTTENLAVFIFDSLKKALLK
PELLYEVKIYETDKNSVIYRGKVKNIYSNGMRRVSANICANMSSDSDS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g4376.t1 | CDD | cd00470 | PTPS | 10 | 141 | 1.00211E-70 |
| 7 | g4376.t1 | Gene3D | G3DSA:3.30.479.10 | - | 2 | 142 | 1.6E-60 |
| 2 | g4376.t1 | PANTHER | PTHR12589 | PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE | 2 | 143 | 7.1E-45 |
| 3 | g4376.t1 | PANTHER | PTHR12589:SF7 | 6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE | 2 | 143 | 7.1E-45 |
| 1 | g4376.t1 | Pfam | PF01242 | 6-pyruvoyl tetrahydropterin synthase | 10 | 141 | 1.8E-33 |
| 6 | g4376.t1 | ProSitePatterns | PS00987 | 6-pyruvoyl tetrahydropterin synthase signature 1. | 39 | 49 | - |
| 5 | g4376.t1 | ProSitePatterns | PS00988 | 6-pyruvoyl tetrahydropterin synthase signature 2. | 85 | 92 | - |
| 4 | g4376.t1 | SUPERFAMILY | SSF55620 | Tetrahydrobiopterin biosynthesis enzymes-like | 8 | 141 | 8.89E-44 |
| 8 | g4376.t1 | TIGRFAM | TIGR00039 | 6PTHBS: 6-pyruvoyl tetrahydropterin synthase/QueD family protein | 8 | 142 | 8.9E-40 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003874 | 6-pyruvoyltetrahydropterin synthase activity | MF |
| GO:0006729 | tetrahydrobiopterin biosynthetic process | BP |
KEGG
Orthology
- This transcript has been annotated with K01737 (6-pyruvoyltetrahydropterin/6-carboxytetrahydropterin synthase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
