Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Probable cytochrome P450 6a20.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g44 g44.t1 TSS g44.t1 405320 405320
chr_3 g44 g44.t1 isoform g44.t1 406315 406626
chr_3 g44 g44.t1 exon g44.t1.exon1 406315 406626
chr_3 g44 g44.t1 cds g44.t1.CDS1 406315 406626
chr_3 g44 g44.t1 TTS g44.t1 406684 406684

Sequences

>g44.t1 Gene=g44 Length=312
ATGGTTCCGATTTTGGCAATTCATCACGATCCACTTTATTATCCAGAACCAAATAATTTT
GATCCCGAGAGATTTGATGAGGAAAAAATTCGACAAAGACCAGCTTTTACATTTTTACCA
TTTGGTGATGGAATGAGAAATTGTATTGGAATGCGATTTGCACTTCTTCAAGCTAAAATT
GCAATAGCAAAACTCATTCTTAATTTTGAGTTTTCAGTTTGTGATAAGACAACAATTCCA
ATAGAATTTAATGCATCAACACCATTTTTAACACCAAAATCTGAAATTTATTTGTCAGTG
AAAAAATTATAA

>g44.t1 Gene=g44 Length=103
MVPILAIHHDPLYYPEPNNFDPERFDEEKIRQRPAFTFLPFGDGMRNCIGMRFALLQAKI
AIAKLILNFEFSVCDKTTIPIEFNASTPFLTPKSEIYLSVKKL

Protein features from InterProScan

Transcript Database ID Name Start End E.value
8 g44.t1 Gene3D G3DSA:1.10.630.10 Cytochrome p450 1 103 1.4E-35
2 g44.t1 PANTHER PTHR24292 CYTOCHROME P450 1 100 4.0E-35
3 g44.t1 PRINTS PR00463 E-class P450 group I signature 3 27 7.5E-12
5 g44.t1 PRINTS PR00463 E-class P450 group I signature 38 48 7.5E-12
4 g44.t1 PRINTS PR00463 E-class P450 group I signature 48 71 7.5E-12
1 g44.t1 Pfam PF00067 Cytochrome P450 1 99 6.7E-31
7 g44.t1 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 41 50 -
6 g44.t1 SUPERFAMILY SSF48264 Cytochrome P450 1 101 8.25E-33

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values