General information for g4405.t13
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative cysteine proteinase CG12163.
Parent gene
- The parent gene g4405 is located on chr_2: 2005157..2008641 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4405 | g4405.t13 | TTS | g4405.t13 | 2001061 | 2001061 |
| chr_2 | g4405 | g4405.t13 | isoform | g4405.t13 | 2001294 | 2003272 |
| chr_2 | g4405 | g4405.t13 | exon | g4405.t13.exon1 | 2001294 | 2001377 |
| chr_2 | g4405 | g4405.t13 | exon | g4405.t13.exon2 | 2001442 | 2001689 |
| chr_2 | g4405 | g4405.t13 | cds | g4405.t13.CDS1 | 2001648 | 2001689 |
| chr_2 | g4405 | g4405.t13 | exon | g4405.t13.exon3 | 2001761 | 2002007 |
| chr_2 | g4405 | g4405.t13 | cds | g4405.t13.CDS2 | 2001761 | 2002007 |
| chr_2 | g4405 | g4405.t13 | exon | g4405.t13.exon4 | 2002061 | 2003089 |
| chr_2 | g4405 | g4405.t13 | cds | g4405.t13.CDS3 | 2002061 | 2003046 |
| chr_2 | g4405 | g4405.t13 | exon | g4405.t13.exon5 | 2003160 | 2003272 |
| chr_2 | g4405 | g4405.t13 | TSS | g4405.t13 | NA | NA |
Sequences
>g4405.t13 Gene=g4405 Length=1721
CGTCAAATCGGTGTTCCCGGTGGAATCAGTCCTGTAGAAAACTTTGAAGATGTAAAAATT
TATGTTCAAGAAGCTATTGATGAAATTAATGATAATGAAGATCCTGATTACATTTTGAAA
CATATCGTTGAAGCAACCCAACAAGTTGTTGCAGGCATGAGTTATAAAATTAAAGCAGTG
TTTTCCAGAGATGGAAGCGACATTGAATGTGATTTTGATGTATGGGAGCAAGCTTGGATT
AAAGATGGACGTAAAGTTTCAGTTTCTTGCAAAAATGATAAGAAATATAAGTTGACCCAA
TCACCATCTAATCAGCGTGTCAAACGTGATAACACGCTTGAAAGAGTTCTTGGTTTACCA
TCCAATACTGATGATCATGACGATTTGATAAAAATACTTTCTGAACATTTGAAGAGACTC
GATACTGGAAGTGATGCACAATTTGAATTGGTAAAACTTGAAAAGGTAACTCAACAAGTA
GTAGCTGGAATAAAATATAAAGCAACAGGTATTTTTAAAATTGGCAATGAAGAGAAAAAA
TGTGTTATCGATGTATGGCATCGCTCATGGATTAAGGGAGATGAAGGCACTCAATTAAGC
GCTGATTGTGATAAAGGTGCAACAACTTTCAAGACAAAATCTTCTAGAAAAAGGAGATCA
GTTCATCACCACACACACAATCGTCACAATAGACAATCAGTAAGCGATCATTTTGATGAC
CATCATCATCATACTGATAGACATCATCATCAATACTCAGCTACTGAAGAAATGAAAGAA
ATAAAATCTGAAATTTTATTTAACAATTTCATAACTAAATATAATCGTAAATATGCCAAT
GAACTTGAACATAAAATGAGAATGAGAATTTTCAAGAAGAATTTACATAAAATTGAAATG
TTGAATAAGCATGAACAAGGCACTGCAAAGTATGGAATTACAGAATTCGCTGATTTAACT
GAAAAGGAATACTTGCATAAAACTGGTTTGAGAGTGCGTGAAAGACATGAGAATGAATTA
GAAAATCCAATTGCACATATTCCAGAAGTTGAAGATTTACCAACCGAATTTGATTGGAGA
GATAAATCAGCAGTTACAAGTGTAAAAAATCAAGGAAATTGTGGATCATGCTGGAGTTTT
TCTGTTACAGGAAATATTGAAGGCTTACATGCTATTAAAACTGGAAAACTTGAAGCTTAT
TCTGAACAAGAACTTTTGGACTGTGATACAACTGATAATGCTTGCAATGGTGGTTATATG
GATGATGCTTTTAAAGCAATTGAAAAAATTGGTGGTCTAGAATTAGAAGATGAATATCCT
TATCAAGCAAGGAAACAAAAGAAATGCTTGTTTAATGCTACTATGAGTCATGTTAAAGTT
AAAGGTGTTATTTGCCTAAAGGTGATGAAATTGCAATGCAAAAGTTTTTAGTCTCAACTG
GTCCGATTTCCATTGGCATAAATGCTAATGCTATGCAATTTTATCGTGGTGGTGTTTCGC
ATCCATGGAAAGTTCTTTGCAGAAAATCTAATTTAGATCATGGTGTTTTGATTGTTGGAT
ATGGAATAAAAGAGTATCCCATGTTTAATAAAACTTTACCTTATTGGACTATTAAAAATT
CATGGGGTCCAAAATGGGGTGAACAAGGATATTATCGAGTTTATCGTGGAGATAACAGTT
GTGGAGTTGCAGAAATGGCAAGCAGCGCAGTACTTGAATAA
>g4405.t13 Gene=g4405 Length=424
MSYKIKAVFSRDGSDIECDFDVWEQAWIKDGRKVSVSCKNDKKYKLTQSPSNQRVKRDNT
LERVLGLPSNTDDHDDLIKILSEHLKRLDTGSDAQFELVKLEKVTQQVVAGIKYKATGIF
KIGNEEKKCVIDVWHRSWIKGDEGTQLSADCDKGATTFKTKSSRKRRSVHHHTHNRHNRQ
SVSDHFDDHHHHTDRHHHQYSATEEMKEIKSEILFNNFITKYNRKYANELEHKMRMRIFK
KNLHKIEMLNKHEQGTAKYGITEFADLTEKEYLHKTGLRVRERHENELENPIAHIPEVED
LPTEFDWRDKSAVTSVKNQGNCGSCWSFSVTGNIEGLHAIKTGKLEAYSEQELLDCDTTD
NACNGGYMDDAFKAIEKIGGLELEDEYPYQARKQKKCLFNATMSHVKVKGVICLKVMKLQ
CKSF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g4405.t13 | CDD | cd02248 | Peptidase_C1A | 302 | 411 | 9.2745E-51 |
| 7 | g4405.t13 | Gene3D | G3DSA:3.10.450.10 | - | 60 | 150 | 5.0E-11 |
| 8 | g4405.t13 | Gene3D | G3DSA:3.90.70.10 | Cysteine proteinases | 159 | 420 | 1.4E-63 |
| 14 | g4405.t13 | MobiDBLite | mobidb-lite | consensus disorder prediction | 157 | 203 | - |
| 15 | g4405.t13 | MobiDBLite | mobidb-lite | consensus disorder prediction | 160 | 178 | - |
| 13 | g4405.t13 | MobiDBLite | mobidb-lite | consensus disorder prediction | 179 | 203 | - |
| 3 | g4405.t13 | PANTHER | PTHR13814:SF16 | CYSTATIN | 214 | 407 | 4.6E-76 |
| 4 | g4405.t13 | PANTHER | PTHR13814 | FETUIN | 214 | 407 | 4.6E-76 |
| 1 | g4405.t13 | Pfam | PF08246 | Cathepsin propeptide inhibitor domain (I29) | 215 | 272 | 2.2E-12 |
| 2 | g4405.t13 | Pfam | PF00112 | Papain family cysteine protease | 301 | 411 | 9.7E-37 |
| 10 | g4405.t13 | ProSitePatterns | PS00139 | Eukaryotic thiol (cysteine) proteases cysteine active site. | 319 | 330 | - |
| 12 | g4405.t13 | SMART | SM00848 | Inhibitor_I29_2 | 215 | 272 | 3.0E-18 |
| 11 | g4405.t13 | SMART | SM00645 | pept_c1 | 301 | 424 | 6.2E-15 |
| 5 | g4405.t13 | SUPERFAMILY | SSF54403 | Cystatin/monellin | 73 | 140 | 3.4E-8 |
| 6 | g4405.t13 | SUPERFAMILY | SSF54001 | Cysteine proteinases | 208 | 415 | 1.23E-62 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008234 | cysteine-type peptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed