General information for g45.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Cytochrome P450 6A1.
Parent gene
- The parent gene g45 is located on chr_3: 406759..407706 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g45 | g45.t1 | isoform | g45.t1 | 406759 | 407706 |
| chr_3 | g45 | g45.t1 | exon | g45.t1.exon1 | 406759 | 407122 |
| chr_3 | g45 | g45.t1 | cds | g45.t1.CDS1 | 406759 | 407122 |
| chr_3 | g45 | g45.t1 | exon | g45.t1.exon2 | 407177 | 407706 |
| chr_3 | g45 | g45.t1 | cds | g45.t1.CDS2 | 407177 | 407706 |
| chr_3 | g45 | g45.t1 | TSS | g45.t1 | NA | NA |
| chr_3 | g45 | g45.t1 | TTS | g45.t1 | NA | NA |
Sequences
>g45.t1 Gene=g45 Length=894
ATGCATCTCTTCATATTTTTTCTTCTTGTATTTTTCACATACATTTATGTATGGTTAAAT
AAGCGAAAAAACTACTGGAAAGATCGCGGTTTTTTACATGAAAAATTTGAATTTCCTTTT
GGCTCATTCAAAGGAATTGGAAGTGAAAAGTCAATGTGTATTGGATTGGATGAGTATTAT
AAAAAATATAAAGGAAAAGGAATGGCTGTCGGGTTATTTTGCTTTGTGAAACCATTGCTA
CTTCCTATTGATCCGGAACTAATAAAAATGATTTGTGTTCAGAACTTTGATGCTTTTCAA
GATCGATTAATTTATTACAATAAAGAAGATGATCCAATTTCAGTTCATTTACTTGCACTT
GAAGGACAAAAATGGAAGGAAAGAAGACAGAAATTAACATTCGTGTTTTCTTCAGCTAAA
ATGAGACTGATGTTTGATATAATTCAATTAATTGGCAATCATTTTGTGCAATCGTTAGAA
AATGCAATTAAAAAGTCTAATCAACTTGAAATGCATGATTGGCTTGCACGTTTTACTACC
GATGTTATCTCTAATGTTGCTTTTGGCATGGATAGTTCTAGCTTAAATAATCCACAAGCT
GATATGCGAAAATATGGCAAAGAAATTCTAAATTTAGGCCCAACTGGTTTTCTTAAATTT
TTGTTCACTTCATCATTTCCTGCGTTGTCTCGCAAACTGCACATTACAGCAAACAAGAAA
AATGTCATTGATTTTTTTTACAACACGTTTAAGAAAAATATTGAACAACGTGAACGAACG
GAAACATTCCGCAAAGATTTTATACAGATTTTACTGGAATTAAAAAAGAGTTCATCATTG
ACAGTTTCCGAACTTGCTGCAGAAAGTTTCATCTTTTTCTTAGGAGTCTGTTAA
>g45.t1 Gene=g45 Length=297
MHLFIFFLLVFFTYIYVWLNKRKNYWKDRGFLHEKFEFPFGSFKGIGSEKSMCIGLDEYY
KKYKGKGMAVGLFCFVKPLLLPIDPELIKMICVQNFDAFQDRLIYYNKEDDPISVHLLAL
EGQKWKERRQKLTFVFSSAKMRLMFDIIQLIGNHFVQSLENAIKKSNQLEMHDWLARFTT
DVISNVAFGMDSSSLNNPQADMRKYGKEILNLGPTGFLKFLFTSSFPALSRKLHITANKK
NVIDFFYNTFKKNIEQRERTETFRKDFIQILLELKKSSSLTVSELAAESFIFFLGVC
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g45.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 14 | 295 | 8.1E-49 |
| 2 | g45.t1 | PANTHER | PTHR24292 | CYTOCHROME P450 | 5 | 294 | 2.2E-78 |
| 3 | g45.t1 | PANTHER | PTHR24292:SF95 | CYP6A16, ISOFORM B-RELATED | 5 | 294 | 2.2E-78 |
| 5 | g45.t1 | PRINTS | PR00464 | Group II E-class P450 signature | 122 | 142 | 1.9E-7 |
| 4 | g45.t1 | PRINTS | PR00464 | Group II E-class P450 signature | 177 | 195 | 1.9E-7 |
| 1 | g45.t1 | Pfam | PF00067 | Cytochrome P450 | 40 | 295 | 3.3E-24 |
| 10 | g45.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 17 | - |
| 11 | g45.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 12 | g45.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 12 | - |
| 13 | g45.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 17 | - |
| 9 | g45.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 18 | 297 | - |
| 7 | g45.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 54 | 294 | 2.36E-36 |
| 6 | g45.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 2 | 19 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0004497 | monooxygenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
