Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Histone H3.3.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g4523 g4523.t7 isoform g4523.t7 3051941 3053346
chr_2 g4523 g4523.t7 exon g4523.t7.exon1 3051941 3052093
chr_2 g4523 g4523.t7 TSS g4523.t7 3052000 3052000
chr_2 g4523 g4523.t7 exon g4523.t7.exon2 3052352 3053346
chr_2 g4523 g4523.t7 cds g4523.t7.CDS1 3052361 3052909
chr_2 g4523 g4523.t7 TTS g4523.t7 3053343 3053343

Sequences

>g4523.t7 Gene=g4523 Length=1148
ACTTGTCCCATTACTGATGAATCATACTCACTATATGTAATTGCGTTTTAATTTTTACTC
TTCTCGTTGAAAGCAAAAAAAAATATTTTGGTGCATTTTTATTGATAACAAAAATTGCAA
GTGTTGATTTTTTGCAATAATAAAGTGTTAAAGTTTTAAAAAATGGTTCGAACTAAACAA
ACCGCACGTAAGTCAACTGCAACAAGAGGCTCGATCAAGTTGCAAAGAATATTGGAAACA
AAAAGAAAAGAACCGAAGCAACATAAGCAATTCATACCAGTCGGGAGGAAGCATGTTAAG
GACACCAATCATGAGAGAAAGAGAAGATATCGTCCTGGAACTCTTGCGTTGAAGGAAATA
AGACGCTATCAAAAGACAACTGAATTATTGCTACGCGTTGCACCGTTTCAAAGACTTGTC
AGAGAAATAAGTGAATCATTTAAATCTGATTTTCGTTATCAAGCAGGAGCTTTGCATGCA
TTACAAGAGGCAGCAGAATCTTATATCATCGGATTGTTTGAGGACACAAATTTGTGTGCA
ATACACGCCAAGCGGTATATTTTTTTGCTGATGCGTAGTAAAAATTTTATGACTCATTCT
TTTTCTTCTATTTCCTTCTACATAGAGTTACAATTATGCCTAACGACATTCGATTGGCTA
TGCGCATCCGAGGTGAAGGAAGATGGGCCAAATAAAGAAAGCATCAAATAACTATTTGTC
GTTATCTCTAACTATATATTTTATTTACACAAAACAAATAATTAATTAATATATATTTGT
TCTTTTTCGTTGGAAAAATACAATGCATTTTTCGAGTAGAAAATTAATTTTCATTTTCAA
ATAATATAAATATTATATTGAGAATATTATTATAAAACATTTATCTTAAGAATGCAATGA
AAAATGAGAAGAACTATGTAAGATAAGAATCGTTAAAAGAAATAGAGACAGAGAGAGAAC
AAATATTTTTTATAAAAAATTTAAAAGAAAACAAAATTATACATTTTAATATTTTATCAT
TTTGGATTTATTTTCATTCAAAAAAGTTTGTTACATTTAAGTGAAACTAAAGTATAAAAG
AAGATTTTTGATGTGAAATAAAACTGTAATTACGTCATGTCTTTCAATAAAAATTTTAAA
GACCATTA

>g4523.t7 Gene=g4523 Length=182
MVRTKQTARKSTATRGSIKLQRILETKRKEPKQHKQFIPVGRKHVKDTNHERKRRYRPGT
LALKEIRRYQKTTELLLRVAPFQRLVREISESFKSDFRYQAGALHALQEAAESYIIGLFE
DTNLCAIHAKRYIFLLMRSKNFMTHSFSSISFYIELQLCLTTFDWLCASEVKEDGPNKES
IK

Protein features from InterProScan

Transcript Database ID Name Start End E.value
8 g4523.t7 Gene3D G3DSA:1.10.20.10 Histone 2 139 5.8E-44
2 g4523.t7 PANTHER PTHR11426 HISTONE H3 1 132 4.0E-41
4 g4523.t7 PRINTS PR00622 Histone H3 signature 3 17 1.7E-11
3 g4523.t7 PRINTS PR00622 Histone H3 signature 112 128 1.7E-11
1 g4523.t7 Pfam PF00125 Core histone H2A/H2B/H3/H4 29 131 8.3E-31
7 g4523.t7 ProSitePatterns PS00959 Histone H3 signature 2. 81 89 -
6 g4523.t7 SMART SM00428 h35 48 144 1.6E-41
5 g4523.t7 SUPERFAMILY SSF47113 Histone-fold 30 131 4.95E-30

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0003677 DNA binding MF
GO:0046982 protein heterodimerization activity MF
GO:0000786 nucleosome CC

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values