General information for g4584.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Hormone-sensitive lipase.
Parent gene
- The parent gene g4584 is located on chr_2: 3336823..3337644 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4584 | g4584.t1 | TTS | g4584.t1 | 3334711 | 3334711 |
| chr_2 | g4584 | g4584.t1 | isoform | g4584.t1 | 3334780 | 3337644 |
| chr_2 | g4584 | g4584.t1 | exon | g4584.t1.exon1 | 3334780 | 3334860 |
| chr_2 | g4584 | g4584.t1 | cds | g4584.t1.CDS1 | 3334780 | 3334860 |
| chr_2 | g4584 | g4584.t1 | exon | g4584.t1.exon2 | 3334925 | 3335435 |
| chr_2 | g4584 | g4584.t1 | cds | g4584.t1.CDS2 | 3334925 | 3335435 |
| chr_2 | g4584 | g4584.t1 | exon | g4584.t1.exon3 | 3335497 | 3335918 |
| chr_2 | g4584 | g4584.t1 | cds | g4584.t1.CDS3 | 3335497 | 3335918 |
| chr_2 | g4584 | g4584.t1 | exon | g4584.t1.exon4 | 3335978 | 3336312 |
| chr_2 | g4584 | g4584.t1 | cds | g4584.t1.CDS4 | 3335978 | 3336312 |
| chr_2 | g4584 | g4584.t1 | exon | g4584.t1.exon5 | 3336366 | 3336393 |
| chr_2 | g4584 | g4584.t1 | cds | g4584.t1.CDS5 | 3336366 | 3336393 |
| chr_2 | g4584 | g4584.t1 | exon | g4584.t1.exon6 | 3336452 | 3336742 |
| chr_2 | g4584 | g4584.t1 | cds | g4584.t1.CDS6 | 3336452 | 3336742 |
| chr_2 | g4584 | g4584.t1 | exon | g4584.t1.exon7 | 3336803 | 3337190 |
| chr_2 | g4584 | g4584.t1 | cds | g4584.t1.CDS7 | 3336803 | 3337190 |
| chr_2 | g4584 | g4584.t1 | exon | g4584.t1.exon8 | 3337250 | 3337644 |
| chr_2 | g4584 | g4584.t1 | cds | g4584.t1.CDS8 | 3337250 | 3337644 |
| chr_2 | g4584 | g4584.t1 | TSS | g4584.t1 | 3337842 | 3337842 |
Sequences
>g4584.t1 Gene=g4584 Length=2451
ATGGAAAGTGATAACGCCTTAAATAAGTCAGCTGGCATTGGAGTGAACTTTGTGATAAGT
AAATCTATGACAGAGAAAAAATATGAAATGGTAAATGAATTGATAAGAATGTGTTCGAGT
AATGTCGAATATTACAAGAATGACAAATCGTTTGTCGGCACGCGGATGCAACATAATTTT
GAAAAGTTAAAAGAATTTTTAGAAAAATCAGTGCCAGTAATTAAAGAAATTGAAAGTTTT
GCTCATCTTTATGATTATGATGAAAATACTCCAGGAAATGGATACCGAAGTTTTGTTCAT
ATTTTTGATTCTGCAGTCAAATATTCACTCAAACACTGTCAATATGTTACTGAAAATCGT
GGAAGTTTACTTTTTAGAAAGAGTCTTTATCAAAAAGAGATTGATGTTTGTTCACAACTT
ATTGAAAGTCTTTATCAAATATGCAGTGATTTAGTAAAAATGCATGAAAATTCGGAAGGT
GGAAACCTTTACTCAAAAGAATGGTCATACGATAATCTTATAACGCGTGGGGCGAAGGTT
ATAAATCAAACATGCTTTTATGGACGAGCTGTTGGTTTTCAATTTGAGCAATCACTAAGA
AGCGTTCTTAAATTCCTTGCTGTTTCACTTGCTTGCTTTTCTGAAGCATATTTCACAAAT
GGCTTCAAATTTATGAAAACTACAAATTATTTCTTAAAGCATCCGTCGTACTTTTTAGAT
CCAGAAATGTGTGCAAGACGTATTATCAATGTCAGTCATAATGCTGATGTAAAATTCTGC
AAGTCATTTTGGACACTTGGTGAAAGTAAATTAATGCATGCACTTCCACGACTTGTTGGT
TACAAAGTAGATGTCAATCGAGTATTTAAAATTCCACCTGAACCACTTCAAATTTATAGT
AAAAAATTTGATAAAATGATTGATATTCCCGTGCCGCATAGTCATATTGGAAAAGATCCA
GTTTCATGTAGACTATTGTGTGCTAGACGCCGTAAAGGAATGGTTGGTGAAAAATTAGCA
GAAGCAAATTTACCAGAACCATCAAAATATTTAATAATTCACACTCATGGCGGTGGATGG
ATTGCTACAAGCTCAAAATCACATGAATTTTACTTAAGAGAATGGGCTGATCGTTTAGAT
TGTCCAGTTCTTTCAATTGATTATCATCTTGCTCCTAAAGCTACTTTTCCTAGAGGGTTA
GAAGACGTTTTCTACACTTATTGTTGGATCTTAAAAAATTGTGAATTATTAGGAACAACT
GCTGAAAATATTGTTTTCTGTGGTGATAGTGCTGGAGCTAATCTTAATACAGCTTGTATT
ATAAAATGCATTGAAATGGGAATTCCAACAGTGAAAGGAATTTTCAATGCATACAGTCCA
TTTTTAGTTAATTTTGCTCAAACGCCTGCTAGATATTTAGTTGTTCAAGACCCTTTGGTT
CCATATGGTTTCATAATGAGAATTTTTAAAACTTATGGTACTCCTGTACCTATCAATGAT
GATAAAGATGAAAAAGAAATTGAAAATGTGTCAGACGAGAAAATTACAGATATTCAAGAT
GAAAAAGATGGTGCAACTTTATTATCACCTGAAAGCTCAAAATCACTAGAAGCAATTTGG
CAGAAAGTGAAAAGCACAGAAGAACCTGATTGGCATTCAAATTTAAATTCCATTCAAGAG
ACATCGTCGGAAGAACCAGTTTCACCGTTGGTCTATCGAAGTGACAGCATAGTTGAGCCT
CAATTTGCTGAAACATCAAACTATGATGATGATGAAGATGCTTTAGAAATGAAAATTGAT
TTAAAGAAAGAAATCACAGATGAAAAGGAAGAAAATTTTGTCAATGATTTTGTTAAAAAA
TATGAGACAACTAGTAGCAATAGTATCATTGATCCAATTATCACACGACCTCGTATGAAC
TCTCGTACATTGAGTGAAGAAAATATTGTCGTTGATGTAGGCATAGATGCATTGAAAATA
GAAAACCTTCAAAATGCTATGACAAACATAAAAAATGCTGTTTCAGGATCAAGTGAAACT
GCTATAATAAGCAGTAGCAATATGAGCAATAAAAATGAAACTTTAAAACGTAATTTTGCA
TTCAGTGAGCATCGTGATAGAAGTGAAGGAATTCCAAAAGCACCTGAAGATGAATTTTTA
TTTACTGTTCCTCGTAATCATTTAATGTCACCTTATATTGCACCAGATGAAATTCTAAGA
CAATTTCCTAAAACAAATATTCTCACGAGCATCACTGATCCATGTATCGATGACTGCGTT
GAAATGGCGAAAAAATTACGCGCTCTTAATGTTAATATTGAACTTGATATTGTCGGCGCA
TTGCCGCATGGGTTTTTAAACTTTGCCCATATATCTAAAGAATGCCATGATGCATCTATG
CTATGTCTGAAGCGAATAGCAACATTGCTTGGAATGAAAAATGAAGTTTAA
>g4584.t1 Gene=g4584 Length=816
MESDNALNKSAGIGVNFVISKSMTEKKYEMVNELIRMCSSNVEYYKNDKSFVGTRMQHNF
EKLKEFLEKSVPVIKEIESFAHLYDYDENTPGNGYRSFVHIFDSAVKYSLKHCQYVTENR
GSLLFRKSLYQKEIDVCSQLIESLYQICSDLVKMHENSEGGNLYSKEWSYDNLITRGAKV
INQTCFYGRAVGFQFEQSLRSVLKFLAVSLACFSEAYFTNGFKFMKTTNYFLKHPSYFLD
PEMCARRIINVSHNADVKFCKSFWTLGESKLMHALPRLVGYKVDVNRVFKIPPEPLQIYS
KKFDKMIDIPVPHSHIGKDPVSCRLLCARRRKGMVGEKLAEANLPEPSKYLIIHTHGGGW
IATSSKSHEFYLREWADRLDCPVLSIDYHLAPKATFPRGLEDVFYTYCWILKNCELLGTT
AENIVFCGDSAGANLNTACIIKCIEMGIPTVKGIFNAYSPFLVNFAQTPARYLVVQDPLV
PYGFIMRIFKTYGTPVPINDDKDEKEIENVSDEKITDIQDEKDGATLLSPESSKSLEAIW
QKVKSTEEPDWHSNLNSIQETSSEEPVSPLVYRSDSIVEPQFAETSNYDDDEDALEMKID
LKKEITDEKEENFVNDFVKKYETTSSNSIIDPIITRPRMNSRTLSEENIVVDVGIDALKI
ENLQNAMTNIKNAVSGSSETAIISSSNMSNKNETLKRNFAFSEHRDRSEGIPKAPEDEFL
FTVPRNHLMSPYIAPDEILRQFPKTNILTSITDPCIDDCVEMAKKLRALNVNIELDIVGA
LPHGFLNFAHISKECHDASMLCLKRIATLLGMKNEV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g4584.t1 | Gene3D | G3DSA:3.40.50.1820 | - | 331 | 532 | 0.0e+00 |
| 7 | g4584.t1 | Gene3D | G3DSA:3.40.50.1820 | - | 682 | 812 | 0.0e+00 |
| 4 | g4584.t1 | PANTHER | PTHR23025 | TRIACYLGLYCEROL LIPASE | 24 | 810 | 0.0e+00 |
| 1 | g4584.t1 | Pfam | PF06350 | Hormone-sensitive lipase (HSL) N-terminus | 31 | 334 | 0.0e+00 |
| 3 | g4584.t1 | Pfam | PF07859 | alpha/beta hydrolase fold | 352 | 465 | 0.0e+00 |
| 2 | g4584.t1 | Pfam | PF07859 | alpha/beta hydrolase fold | 724 | 786 | 5.9e-05 |
| 5 | g4584.t1 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 349 | 790 | 0.0e+00 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016042 | lipid catabolic process | BP |
| GO:0016787 | hydrolase activity | MF |
| GO:0008203 | cholesterol metabolic process | BP |
| GO:0016298 | lipase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K07188 (hormone-sensitive lipase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
