Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Lactosylceramide 4-alpha-galactosyltransferase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g4600 g4600.t1 isoform g4600.t1 3454678 3455841
chr_2 g4600 g4600.t1 exon g4600.t1.exon1 3454678 3455029
chr_2 g4600 g4600.t1 cds g4600.t1.CDS1 3454678 3455029
chr_2 g4600 g4600.t1 exon g4600.t1.exon2 3455083 3455494
chr_2 g4600 g4600.t1 cds g4600.t1.CDS2 3455083 3455494
chr_2 g4600 g4600.t1 exon g4600.t1.exon3 3455583 3455841
chr_2 g4600 g4600.t1 cds g4600.t1.CDS3 3455583 3455841
chr_2 g4600 g4600.t1 TSS g4600.t1 NA NA
chr_2 g4600 g4600.t1 TTS g4600.t1 NA NA

Sequences

>g4600.t1 Gene=g4600 Length=1023
ATGAATCACAATAAACTCAAAAAAATTTTAATTTTAATTCTTATAAAATTTCTCATAGTT
TTTATAATTCATCGATTTTATTTATATCAGCAATGGTTTCAAGACGAGTGCAATAAAAAT
ACTGCAATTATAATTGAAAATAATTTTAATCAAGTTCCTATTGAAATGCTAAAAAACGAA
ACAATTTATTTTCTTGAAACACACAATTCAAGTGATCATGAACTCACAGCTAGACAGGCA
TGTGCTATAGAATCAGCAGCTTATATGAATCCAAACACTAACGTTTTTGTTCTTTTTTTA
TCGTCATCAAATCACATTCGTCTCAAAAATTGGTCACAATTTCAAAGAATTTTAGATTAT
AAAAATATTCATTTAAAGTATTTTCATATGGATGATATAATAAAAGGTACTGTAGTTGAG
GATTTTTTGACTCATGATATGATACTAAATTCAAAATGGAAAGAACTTCATATAAGTGAC
ATATTAAGATATGCTTTGCTTCGTACATATTCGGGAGTATATTTAGATTTAGACATAATT
GTGAAAAGGCCGTTAGATGAAATTAAACTAAAGAACTTTGTCTGTCTGGAAGATAAAAAC
AGTATTAACAATGCAATAATAAAGTTTAGTGATGTTGATATTGCTTACAAAATAGGCAAT
TGGTTACTTGAGGGTGTGATAAAAAATTTCGATGGAAAACAATGGACAATTGGACCGAGA
CTCTTAACAAAAGTCATAAAAAGATATTGCAATATTACTGATTTTATAAACACAACAGAA
TGCAATGGAATAAATATCTTACCAATTGAAAAATGTTATGCAATAGAAGGATATCAATGG
AAATATTTTATTCAAGAATTTCGTTCAGATCAAGTTATGAGTGCTGTAGAGAATTCACAT
TTTGTTCATCTTTGGAATTATCAAAGCGACAAAAGACTTGTTAAAACTGGAAAATCTGTA
GCATTAAATTTTTTAGCAGCGGAGTATTGTCCACTAGTATTTGAATCAAATTTATACATT
TAG

>g4600.t1 Gene=g4600 Length=340
MNHNKLKKILILILIKFLIVFIIHRFYLYQQWFQDECNKNTAIIIENNFNQVPIEMLKNE
TIYFLETHNSSDHELTARQACAIESAAYMNPNTNVFVLFLSSSNHIRLKNWSQFQRILDY
KNIHLKYFHMDDIIKGTVVEDFLTHDMILNSKWKELHISDILRYALLRTYSGVYLDLDII
VKRPLDEIKLKNFVCLEDKNSINNAIIKFSDVDIAYKIGNWLLEGVIKNFDGKQWTIGPR
LLTKVIKRYCNITDFINTTECNGINILPIEKCYAIEGYQWKYFIQEFRSDQVMSAVENSH
FVHLWNYQSDKRLVKTGKSVALNFLAAEYCPLVFESNLYI

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g4600.t1 Gene3D G3DSA:3.90.550.20 - 72 258 8.2E-11
3 g4600.t1 PANTHER PTHR12042 LACTOSYLCERAMIDE 4-ALPHA-GALACTOSYLTRANSFERASE ALPHA- 1,4-GALACTOSYLTRANSFERASE 41 335 1.6E-53
1 g4600.t1 Pfam PF04488 Glycosyltransferase sugar-binding region containing DXD motif 77 190 3.3E-13
2 g4600.t1 Pfam PF04572 Alpha 1,4-glycosyltransferase conserved region 222 335 2.0E-23
7 g4600.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 8 -
9 g4600.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 9 29 -
8 g4600.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 30 340 -
5 g4600.t1 SUPERFAMILY SSF53448 Nucleotide-diphospho-sugar transferases 71 333 7.17E-22
4 g4600.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 9 28 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed