General information for g4611.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 4g15.
Parent gene
- The parent gene g4611 is located on chr_2: 3553893..3559094 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4611 | g4611.t1 | TSS | g4611.t1 | 3552667 | 3552667 |
| chr_2 | g4611 | g4611.t1 | isoform | g4611.t1 | 3552770 | 3559094 |
| chr_2 | g4611 | g4611.t1 | exon | g4611.t1.exon1 | 3552770 | 3552986 |
| chr_2 | g4611 | g4611.t1 | cds | g4611.t1.CDS1 | 3552770 | 3552986 |
| chr_2 | g4611 | g4611.t1 | exon | g4611.t1.exon2 | 3553893 | 3554090 |
| chr_2 | g4611 | g4611.t1 | cds | g4611.t1.CDS2 | 3553893 | 3554090 |
| chr_2 | g4611 | g4611.t1 | exon | g4611.t1.exon3 | 3554640 | 3554822 |
| chr_2 | g4611 | g4611.t1 | cds | g4611.t1.CDS3 | 3554640 | 3554822 |
| chr_2 | g4611 | g4611.t1 | exon | g4611.t1.exon4 | 3557332 | 3557852 |
| chr_2 | g4611 | g4611.t1 | cds | g4611.t1.CDS4 | 3557332 | 3557852 |
| chr_2 | g4611 | g4611.t1 | exon | g4611.t1.exon5 | 3558389 | 3558442 |
| chr_2 | g4611 | g4611.t1 | cds | g4611.t1.CDS5 | 3558389 | 3558442 |
| chr_2 | g4611 | g4611.t1 | exon | g4611.t1.exon6 | 3558501 | 3558626 |
| chr_2 | g4611 | g4611.t1 | cds | g4611.t1.CDS6 | 3558501 | 3558626 |
| chr_2 | g4611 | g4611.t1 | exon | g4611.t1.exon7 | 3558678 | 3559094 |
| chr_2 | g4611 | g4611.t1 | cds | g4611.t1.CDS7 | 3558678 | 3559094 |
| chr_2 | g4611 | g4611.t1 | TTS | g4611.t1 | 3559231 | 3559231 |
Sequences
>g4611.t1 Gene=g4611 Length=1716
ATGGCTGGTGGAACAGTTTCAGCCGATCAAGTTCAATCGCAAACTCAAGTCAGCTCGACT
ACTATTTTCATTTCTCTTTTACTTCCGGCATTATTGCTTTGGTACGTTTATTGGAAATTA
TCACGTAGACATCTCTATAAACTCGCTGAGAAATTACCGGGACCAAAGGGATTGCCTTTT
ATAGGAAATGCATTAGATCTAACTGGAACATCACATATTGTCTTTAAAACAGTCACAGAA
AAAGGAAAAGAATTTAATCAAGTAATCAAACTATGGGCTGGTCCAAAACTTCTTGTTTTC
TTGGTTGATCCTCGTGATATTGAACTTGTATTGAGCAGTCAAGTTTATCTTGAAAAATCA
CCAGAGTATAGATTTTTTGAACCATGGTTAGGTCAAGGATTGTTAATTAGTTCAGGTCAA
AAATGGCGAGCCCATCGTAAATTAATTGCACCAACTTTTCATCTAAATGTTTTGAAAAGT
TTCATTGAACTCTTTAATGATAACTCGCGACTTGTATGCAAAAAAATGCTAGCATTGAAT
GGTAAAACTTTTGATTGTCATGATTATATGTCAGAATGTACAGTCGAAATTCTTTTGGAA
ACGGCAATGGGTGTCTCGCGCAAGACTCAAGATCAATCTGGTTTCGATTATGCAATGGCT
GTTATGAAAATGTGCGATATTCTGCATCTTCGTCATACAAAGCTTTGGCTTTATCCTGAT
TTTCTTTTTAAACTCTCAAAATACTCGAAACTTCAAGTCAACCTTTTGGATAAAATTCAC
AGTCTTACAAAGAAAGTGATTAAAAGTAAAAAAGAAGCTTTTTCAAAAGGAACACGTGGA
ACTTTGGCAACACCAGAATTCATTAAGCCTGAAGAGAAACAAGAGAAGTCAAAGGAAGAA
AAGAAAGCAGAAACAACAGTTGTAGAAGGACTTTCATTTGGACAATCTGCTGGATTAAAA
GATGATTTAGATGTTGATGATGGTATGGATGTTGGTGAAAAGAAACGATTGGCTTTCTTA
GATTTATTATTGGAAAGTGCTGAAAATGGTGCAGTTATATCTGAACAAGAAATTAAAGAA
CAAGTGGATACAATCATGTTTGAGGGCCATGACACGACGGCTGCGGGCTCAAGCTTCTTC
TTATCACTTATGGGCATTCATCAAGATATTCAAGATAAAGTTATTGAAGAAATTGATTCA
ATTTTTGGAGACTCTGATCGTCCTTGCACATTCCAAGATACTTTAGAGATGAAATACCTT
GAACGTTGTATGATGGAAACCTTGAGAATGTATCCACCGGTACCAGTTATTGCAAGACAC
ATGAAACAAGATTTGAAACTTGCATCAGGCGATTATACTGTTCCAAAAGGATCAACTGTT
ATTGTGGCAACTTATAAACTTCATCGGTTGGAATCAATCTACCCAAATCCAGATAAATTC
GATCCTGACAACTTTTTGCCTGAACGAACCGCAAACCGTCACTATTATGCATTTGTGCCT
TTCTCAGCTGGTCCGAGATCTTGCGTTGGACGTAAATATGCGATGCTCAAATTAAAAGTC
ATACTTTCAACAATTCTTCGTCAATTCCGTGTTTATTCAGATTTGGATGAAAAAGACTTT
CGTTTACAAGCTGATATAATTTTGAAACGCGAAGAAGGCTTCCAAATTCGTCTAGAACCA
CGCAAACCTAAATCAAAACAAGCAGCTGCTTCATAG
>g4611.t1 Gene=g4611 Length=571
MAGGTVSADQVQSQTQVSSTTIFISLLLPALLLWYVYWKLSRRHLYKLAEKLPGPKGLPF
IGNALDLTGTSHIVFKTVTEKGKEFNQVIKLWAGPKLLVFLVDPRDIELVLSSQVYLEKS
PEYRFFEPWLGQGLLISSGQKWRAHRKLIAPTFHLNVLKSFIELFNDNSRLVCKKMLALN
GKTFDCHDYMSECTVEILLETAMGVSRKTQDQSGFDYAMAVMKMCDILHLRHTKLWLYPD
FLFKLSKYSKLQVNLLDKIHSLTKKVIKSKKEAFSKGTRGTLATPEFIKPEEKQEKSKEE
KKAETTVVEGLSFGQSAGLKDDLDVDDGMDVGEKKRLAFLDLLLESAENGAVISEQEIKE
QVDTIMFEGHDTTAAGSSFFLSLMGIHQDIQDKVIEEIDSIFGDSDRPCTFQDTLEMKYL
ERCMMETLRMYPPVPVIARHMKQDLKLASGDYTVPKGSTVIVATYKLHRLESIYPNPDKF
DPDNFLPERTANRHYYAFVPFSAGPRSCVGRKYAMLKLKVILSTILRQFRVYSDLDEKDF
RLQADIILKREEGFQIRLEPRKPKSKQAAAS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 18 | g4611.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 35 | 313 | 1.3E-37 |
| 17 | g4611.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 319 | 566 | 1.4E-77 |
| 25 | g4611.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 285 | 304 | - |
| 24 | g4611.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 286 | 304 | - |
| 3 | g4611.t1 | PANTHER | PTHR24291:SF106 | CYTOCHROME P450 4G1-RELATED | 11 | 560 | 3.9E-282 |
| 4 | g4611.t1 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 11 | 560 | 3.9E-282 |
| 5 | g4611.t1 | PRINTS | PR00463 | E-class P450 group I signature | 82 | 101 | 5.6E-22 |
| 11 | g4611.t1 | PRINTS | PR00463 | E-class P450 group I signature | 357 | 374 | 5.6E-22 |
| 13 | g4611.t1 | PRINTS | PR00385 | P450 superfamily signature | 368 | 385 | 1.2E-7 |
| 9 | g4611.t1 | PRINTS | PR00463 | E-class P450 group I signature | 377 | 403 | 5.6E-22 |
| 7 | g4611.t1 | PRINTS | PR00463 | E-class P450 group I signature | 421 | 439 | 5.6E-22 |
| 15 | g4611.t1 | PRINTS | PR00385 | P450 superfamily signature | 422 | 433 | 1.2E-7 |
| 10 | g4611.t1 | PRINTS | PR00463 | E-class P450 group I signature | 463 | 487 | 5.6E-22 |
| 8 | g4611.t1 | PRINTS | PR00463 | E-class P450 group I signature | 498 | 508 | 5.6E-22 |
| 12 | g4611.t1 | PRINTS | PR00385 | P450 superfamily signature | 499 | 508 | 1.2E-7 |
| 6 | g4611.t1 | PRINTS | PR00463 | E-class P450 group I signature | 508 | 531 | 5.6E-22 |
| 14 | g4611.t1 | PRINTS | PR00385 | P450 superfamily signature | 508 | 519 | 1.2E-7 |
| 1 | g4611.t1 | Pfam | PF00067 | Cytochrome P450 | 53 | 306 | 4.1E-32 |
| 2 | g4611.t1 | Pfam | PF00067 | Cytochrome P450 | 332 | 556 | 2.5E-69 |
| 20 | g4611.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 19 | - |
| 21 | g4611.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 20 | 38 | - |
| 19 | g4611.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 39 | 571 | - |
| 23 | g4611.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 501 | 510 | - |
| 16 | g4611.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 53 | 561 | 7.99E-114 |
| 22 | g4611.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 21 | 38 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K15001 (cytochrome P450 family 4 [EC:1.14.-.-])
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed