General information for g47.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable cytochrome P450 6a13.
Parent gene
- The parent gene g47 is located on chr_3: 408986..410519 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g47 | g47.t1 | TSS | g47.t1 | 408948 | 408948 |
| chr_3 | g47 | g47.t1 | isoform | g47.t1 | 408986 | 410519 |
| chr_3 | g47 | g47.t1 | exon | g47.t1.exon1 | 408986 | 409109 |
| chr_3 | g47 | g47.t1 | cds | g47.t1.CDS1 | 408986 | 409109 |
| chr_3 | g47 | g47.t1 | exon | g47.t1.exon2 | 409317 | 409335 |
| chr_3 | g47 | g47.t1 | cds | g47.t1.CDS2 | 409317 | 409335 |
| chr_3 | g47 | g47.t1 | exon | g47.t1.exon3 | 409391 | 410519 |
| chr_3 | g47 | g47.t1 | cds | g47.t1.CDS3 | 409391 | 410519 |
| chr_3 | g47 | g47.t1 | TTS | g47.t1 | 410706 | 410706 |
Sequences
>g47.t1 Gene=g47 Length=1272
ATGGATTTAATTTTAACTGTGTTTTTGCTTACAATACTTAGTCTTTTGTATTTGTGGGTT
AAAAGACGTTTTAGTTATTGGAAAGATCTTGGTTTTTTGCAAGCTGAAGCATCATTTCCT
TTCGATGATCCATTGAGTGCTAACATTCTCACGCTTGAAGGTCAAGAATGGCGGGATCGA
AGAGTGAAATTATCGCCTATTTTTACATCAGCAAAAATGAAAATGATGTTTGAAATTATT
GATTCAATTGGAGATAAATTTGTAAATTCACTTGAAAAAGATATGAAACTTTCAAATATT
TGTGAAATGCGTCAATGGTGTCAACGATTTACCAGTGATAACATCGCTAGCACTGCCTTT
GGACTGCAATCGAATTGCCTTGAAGATTCAAATTCACAATTTATGAAAATGGGCAGAAGA
CTTTTTGATCTTTCTACTTTTGAAGTTATAAAATTTTTTGCTTCTACAGCTTGGCCTGAT
TTATCTAGAAAACTTGGAATTTTATTTACTCCAAAAGAAGCAGGTGATTTCTTCCATCAT
ACTTTTCTTCAAACATTTGAAAATCGAACGAAAAATCCAATTGAACGTCATGATTTTGTT
CAATTGCTGTTAAATTTAAAAGACACATTTACACCTACTGAATTGGCAGCAGAAGCATTT
TTGGTTTATGCAGGTGGTTTTGAATCAAGTTCAACATTGATGCAGTTTACACTTTATGAG
CTTGCATTGAATCAAGACATGCAAGATCGTTTGAGAAAAGAATTGATTGAAGGAATTGAA
GAAAATGATGGAAAATTGACTTATGATTTGATTTTTAGCTTCAAATATCTAAATATGGTT
ATTCAGGAATCTCTAAGAAAATATCCACCAATTCCAAATACATTCCGAAAAGCCACAAAA
GACTATCAATTTCCTGACACAAATTTAATTATCCCAAAAGGAACGAATATTATTTTAAAC
ACATATTCGTTACATCGTGATCCAGAGTACTTCCCAGAACCTGAAAAATTTGATCCAGAA
AGGTTTAGTGCTGAAAATGTGAGAAATATTCGGCCGTATACAATGCTTCCTTTCGCAACA
GGTCCACGCAATTGTTTGGGTGAAAGATTCGCTCTTATGCAATCAAAAATTGGAATTACA
AAACTAATAACAAATTTTGTTTTCATGCCATGTGACAAAACAACTATTCCAATGAAATAT
GTTCCAAGTGCACCGTTTTTGGCACCTGTAAATGGAATTCAATTAAAAGTTGAGAAGTTT
AAGAAAGATTAA
>g47.t1 Gene=g47 Length=423
MDLILTVFLLTILSLLYLWVKRRFSYWKDLGFLQAEASFPFDDPLSANILTLEGQEWRDR
RVKLSPIFTSAKMKMMFEIIDSIGDKFVNSLEKDMKLSNICEMRQWCQRFTSDNIASTAF
GLQSNCLEDSNSQFMKMGRRLFDLSTFEVIKFFASTAWPDLSRKLGILFTPKEAGDFFHH
TFLQTFENRTKNPIERHDFVQLLLNLKDTFTPTELAAEAFLVYAGGFESSSTLMQFTLYE
LALNQDMQDRLRKELIEGIEENDGKLTYDLIFSFKYLNMVIQESLRKYPPIPNTFRKATK
DYQFPDTNLIIPKGTNIILNTYSLHRDPEYFPEPEKFDPERFSAENVRNIRPYTMLPFAT
GPRNCLGERFALMQSKIGITKLITNFVFMPCDKTTIPMKYVPSAPFLAPVNGIQLKVEKF
KKD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g47.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 14 | 423 | 3.2E-113 |
| 2 | g47.t1 | PANTHER | PTHR24292 | CYTOCHROME P450 | 42 | 419 | 5.3E-129 |
| 3 | g47.t1 | PANTHER | PTHR24292:SF95 | CYP6A16, ISOFORM B-RELATED | 42 | 419 | 5.3E-129 |
| 5 | g47.t1 | PRINTS | PR00463 | E-class P450 group I signature | 214 | 231 | 3.9E-18 |
| 10 | g47.t1 | PRINTS | PR00385 | P450 superfamily signature | 225 | 242 | 1.8E-10 |
| 7 | g47.t1 | PRINTS | PR00463 | E-class P450 group I signature | 234 | 260 | 3.9E-18 |
| 8 | g47.t1 | PRINTS | PR00463 | E-class P450 group I signature | 278 | 296 | 3.9E-18 |
| 11 | g47.t1 | PRINTS | PR00385 | P450 superfamily signature | 279 | 290 | 1.8E-10 |
| 9 | g47.t1 | PRINTS | PR00463 | E-class P450 group I signature | 320 | 344 | 3.9E-18 |
| 4 | g47.t1 | PRINTS | PR00463 | E-class P450 group I signature | 355 | 365 | 3.9E-18 |
| 13 | g47.t1 | PRINTS | PR00385 | P450 superfamily signature | 356 | 365 | 1.8E-10 |
| 6 | g47.t1 | PRINTS | PR00463 | E-class P450 group I signature | 365 | 388 | 3.9E-18 |
| 12 | g47.t1 | PRINTS | PR00385 | P450 superfamily signature | 365 | 376 | 1.8E-10 |
| 1 | g47.t1 | Pfam | PF00067 | Cytochrome P450 | 44 | 416 | 6.2E-87 |
| 18 | g47.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 19 | g47.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 20 | g47.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 13 | - |
| 21 | g47.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 18 | - |
| 17 | g47.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 423 | - |
| 15 | g47.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 41 | 418 | 2.1E-96 |
| 14 | g47.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 3 | 20 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
