Gene loci information

Transcript annotation

  • This transcript has been annotated as Probable cytochrome P450 6a13.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g48 g48.t8 TSS g48.t8 410853 410853
chr_3 g48 g48.t8 isoform g48.t8 410889 412637
chr_3 g48 g48.t8 exon g48.t8.exon1 410889 411235
chr_3 g48 g48.t8 cds g48.t8.CDS1 410889 411235
chr_3 g48 g48.t8 exon g48.t8.exon2 411293 411525
chr_3 g48 g48.t8 cds g48.t8.CDS2 411293 411525
chr_3 g48 g48.t8 exon g48.t8.exon3 411582 411882
chr_3 g48 g48.t8 cds g48.t8.CDS3 411582 411882
chr_3 g48 g48.t8 exon g48.t8.exon4 411941 412338
chr_3 g48 g48.t8 cds g48.t8.CDS4 411941 412338
chr_3 g48 g48.t8 exon g48.t8.exon5 412395 412417
chr_3 g48 g48.t8 cds g48.t8.CDS5 412395 412417
chr_3 g48 g48.t8 exon g48.t8.exon6 412477 412637
chr_3 g48 g48.t8 cds g48.t8.CDS6 412477 412635
chr_3 g48 g48.t8 TTS g48.t8 412694 412694

Sequences

>g48.t8 Gene=g48 Length=1463
ATGGGTTTTGTTTTGACATTAATAATTATTTTAGTAATTTCATTTTATTTTTGGATGAAA
AAACATTTTAATTATTGGAAAAATCGAGGTTTTCTGCAAGTTGATGGATCATTTCCATTA
GGCTCACTTAGTGGTGTTGGAACAAAAATATGTTCGACTGAAAAATATGACTTGATTTAT
AAAGGTTTCAAGGGAAAAGCGTCGGTTGTTGGATTTTATAATTTTTTATCACCAGCAGTT
TTGCCAATTGAACCTGAATTGATTAAAAATATTTTGGTATTAAATTTTGCATCATTTCAT
GATCGTGGTCTTTATTATAATAAGAAAGATGATCCAATGAGTGCTAATTTAGTAGCTTTA
GAAGGTCAAGAATGGAAAGAACGTCGTACAAAAATCACCCCATTATTTTCATCAGGAAAG
ATTAAAATGATGTTTGAAATAGTCACTGAAATTGGTGATAAATTAGTAAATGCTATAAAA
AGAGAATTGAATGAGTCAACTGAACAAGATATGAGAGTTTGGGCACAAAAATATACAAAT
GATACAATTAGCAATATCGCATTTGGACTCTCTCTTTCTTGTCTTGATGGTGAAAATTTG
GAATTTTTAAAAAATGGAAAGAGACTTTTTGAGCTTAAACCATGGGAAGTTTTGAAAATT
CTTTTTACATCTGAAATGCCAAAACTTAGTAGATTTCTTCGTTTGAAATTCACTGCTGGA
AATACTGGAGAATTTTTTCATAAAACTTTTCTTGAAACTTTTAATTATCGTCAAAATAAT
TCAATTGAACGCAATGATTTAGTGTCGTTGTTGTTAAAACTGAAAGATGTTTATACTCTG
ACAGAACTTGCAGCTGAAGCATTTTTAGTATACACTGGCGGATTTGAGACAAGTTCAACT
TTAATAACTTTTGCTCTCTATGAATTTTCATTAAATCAACAAATTCAAGAAAGATTGCGT
GATGAAATAAATTCGGGAATTGAAAATAATGATGGAAAATTGACTTATGAATTATTAAAC
AGTTTTAAATATTTAGAAATGGTTATTAATGAAACTCTAAGGAAATATCCACCGCTTTCT
AACATTTTTCGTAAAACTACCAAAGATTATAAAATTCCTGATGAAGATTTATTGATTTGT
AAAGGAACAGCAGTTATAATTAATACTTATTCATTACATCATGATCCTCAATACTTTTCA
AAACCTGACAAATTTGATCCGGAAAGGTTTAATGAAGAAAACATTGGAAGTATTAAACCT
TATACATTTTTGCCATTTGGTGCTGGAGCTAGAAATTGCATTGGAATGCGATTTGGAATG
ATGCAAACAAAAATAGCAATCACGAAATTGATTTCAAATTTTTCTTTTAAGCCATGTTCA
AAAACAACAATTCCAATGAAATTTAAACCTTCTTCACCATTCATTTGTCCTGTTGATGGA
ATGTTTTTGAGTGTGGAATCATT

>g48.t8 Gene=g48 Length=487
MGFVLTLIIILVISFYFWMKKHFNYWKNRGFLQVDGSFPLGSLSGVGTKICSTEKYDLIY
KGFKGKASVVGFYNFLSPAVLPIEPELIKNILVLNFASFHDRGLYYNKKDDPMSANLVAL
EGQEWKERRTKITPLFSSGKIKMMFEIVTEIGDKLVNAIKRELNESTEQDMRVWAQKYTN
DTISNIAFGLSLSCLDGENLEFLKNGKRLFELKPWEVLKILFTSEMPKLSRFLRLKFTAG
NTGEFFHKTFLETFNYRQNNSIERNDLVSLLLKLKDVYTLTELAAEAFLVYTGGFETSST
LITFALYEFSLNQQIQERLRDEINSGIENNDGKLTYELLNSFKYLEMVINETLRKYPPLS
NIFRKTTKDYKIPDEDLLICKGTAVIINTYSLHHDPQYFSKPDKFDPERFNEENIGSIKP
YTFLPFGAGARNCIGMRFGMMQTKIAITKLISNFSFKPCSKTTIPMKFKPSSPFICPVDG
MFLSVES

Protein features from InterProScan

Transcript Database ID Name Start End E.value
14 g48.t8 Gene3D G3DSA:1.10.630.10 Cytochrome p450 14 487 1.5E-124
2 g48.t8 PANTHER PTHR24292 CYTOCHROME P450 4 486 3.7E-156
3 g48.t8 PANTHER PTHR24292:SF95 CYP6A16, ISOFORM B-RELATED 4 486 3.7E-156
8 g48.t8 PRINTS PR00463 E-class P450 group I signature 282 299 2.4E-19
9 g48.t8 PRINTS PR00385 P450 superfamily signature 293 310 1.0E-10
4 g48.t8 PRINTS PR00463 E-class P450 group I signature 302 328 2.4E-19
12 g48.t8 PRINTS PR00385 P450 superfamily signature 347 358 1.0E-10
7 g48.t8 PRINTS PR00463 E-class P450 group I signature 388 412 2.4E-19
6 g48.t8 PRINTS PR00463 E-class P450 group I signature 423 433 2.4E-19
10 g48.t8 PRINTS PR00385 P450 superfamily signature 424 433 1.0E-10
5 g48.t8 PRINTS PR00463 E-class P450 group I signature 433 456 2.4E-19
11 g48.t8 PRINTS PR00385 P450 superfamily signature 433 444 1.0E-10
1 g48.t8 Pfam PF00067 Cytochrome P450 68 475 1.8E-94
16 g48.t8 Phobius SIGNAL_PEPTIDE Signal peptide region 1 19 -
17 g48.t8 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 2 -
18 g48.t8 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 3 14 -
19 g48.t8 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 15 19 -
15 g48.t8 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 20 487 -
21 g48.t8 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 426 435 -
13 g48.t8 SUPERFAMILY SSF48264 Cytochrome P450 65 485 2.62E-107
20 g48.t8 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 2 19 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values