Gene loci information

Transcript annotation

  • This transcript has been annotated as Cytochrome P450 4d2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g4831 g4831.t1 TSS g4831.t1 5163901 5163901
chr_2 g4831 g4831.t1 isoform g4831.t1 5163915 5166095
chr_2 g4831 g4831.t1 exon g4831.t1.exon1 5163915 5164074
chr_2 g4831 g4831.t1 cds g4831.t1.CDS1 5163915 5164074
chr_2 g4831 g4831.t1 exon g4831.t1.exon2 5164169 5165322
chr_2 g4831 g4831.t1 cds g4831.t1.CDS2 5164169 5165322
chr_2 g4831 g4831.t1 exon g4831.t1.exon3 5165859 5165876
chr_2 g4831 g4831.t1 cds g4831.t1.CDS3 5165859 5165876
chr_2 g4831 g4831.t1 exon g4831.t1.exon4 5165946 5166095
chr_2 g4831 g4831.t1 cds g4831.t1.CDS4 5165946 5166095
chr_2 g4831 g4831.t1 TTS g4831.t1 5166128 5166128

Sequences

>g4831.t1 Gene=g4831 Length=1482
ATGTGGTTCATTTACGTTGCTCTGTTTATTTTATTTATTTTTATTCTCGATGATTTTCTA
CTTGATAAGCGGCAATTTAAATTAGCTGAAGTTTTCAATGGACCTAAAAGATTGCCACTT
ATTGGAAATGTTAACATTTTCTTAAACCTTAAACCTAAAGACTATTATGATTTTGTAAAG
GATTTTATGGAAAAATATGCACGCACTGGAACTCGTATGGCACGATATTGGGCTGGAAAT
CAACTTGAGTTGATGGTTGATGACTACAAGTTACTAGAGAAAATTATGTCAAATCCAAAG
TTCATTTCAAAAAGTTCTCAATATGGATTTTTGCATGGAGTGTTGGGTGAAGGACTTTTA
TTTAGCACAGATCAAAAATGGTTCAATCGTCGCAGAATCATCACACCAACTTTTCATTTT
AAAATTCTTGAGCAATTTTTTGAAGTTTTTCGTAAACACAATGAATTGTTAATGGAAGAA
ATCAAAGAAAAATCAAAGAATGGAAAACCAATCAACATTTTTCCAATTGTAACTTCTTCT
GTGCTTAATGCACTTTGTGAAACTGCAATGGGTTATGAAATGAAACCACAAGATTCTGAT
TATTTAAATGCTGTTCGTGAAGTTGGTCATATTGTTGCTACAAGATTCTTAAGTCCTTGG
CTAAGAATTGAATTTATTTACAATATGACAGAAGGAAAAAGAAAGCAAGACAAATGTGCT
AAAATTATGCATGAATTTACAACAAAATTGATTGAAGAACGAAGAAAATTGTTATCTAAA
AGTGAAAAGCAGAATTTATCAAATTTAGATGATGATGATATTGGTTTAAAGAAGAAAATG
TGCTTACTTGATGTTTTACTGCAGTCAACTGTTGACAATAAACCATTAAGTAATGCAGAC
ATTCAAGAAGAAGTTGACACTTTTACTTTTGCCGGACATGATACAACCACAAATTGCATC
TGTTTCACACTTTATTTAATTTCAAAACATCCAGAAGTAAAGGAAAAATTATTGAAAGAA
ATTGAAGAAGTTATTGGTGATGGTGAAGTAACTTTTAAAAGTTTAAATGAATTTAAATAT
CTTGAATTAGTCATTAAAGAATCACTTAGACTTTATCCACCTGCACCAGTGATTTCACGA
AGATTATATGAAGAAGTTGATTTTGGTGATTTTGTCGCTCCAGCTAATGCAAATTACAAT
CTTTGTCTTTATACACTCTTCCGTAATTCTGAAGTTTTTGAGAAGCCAAATGAATTTATT
CCTGAGAGATTTTTGAAAAATATTCCACCATTTGCTTTTGTGCCATTTAGTGCGGGTCAA
AGGAATTGTATTGGTCAAAAGTTTGCATTGATAAACATCAAAAACAATTTAATCAACATT
TTACGAAATTTTGAGGTAAATCCAGGAAATGTTGAACCAACATTTGAAATTAATTTGACA
ATTAAATGTGATTCGATGCTTGTCGGTTTTAAACCAAAATAA

>g4831.t1 Gene=g4831 Length=493
MWFIYVALFILFIFILDDFLLDKRQFKLAEVFNGPKRLPLIGNVNIFLNLKPKDYYDFVK
DFMEKYARTGTRMARYWAGNQLELMVDDYKLLEKIMSNPKFISKSSQYGFLHGVLGEGLL
FSTDQKWFNRRRIITPTFHFKILEQFFEVFRKHNELLMEEIKEKSKNGKPINIFPIVTSS
VLNALCETAMGYEMKPQDSDYLNAVREVGHIVATRFLSPWLRIEFIYNMTEGKRKQDKCA
KIMHEFTTKLIEERRKLLSKSEKQNLSNLDDDDIGLKKKMCLLDVLLQSTVDNKPLSNAD
IQEEVDTFTFAGHDTTTNCICFTLYLISKHPEVKEKLLKEIEEVIGDGEVTFKSLNEFKY
LELVIKESLRLYPPAPVISRRLYEEVDFGDFVAPANANYNLCLYTLFRNSEVFEKPNEFI
PERFLKNIPPFAFVPFSAGQRNCIGQKFALINIKNNLINILRNFEVNPGNVEPTFEINLT
IKCDSMLVGFKPK

Protein features from InterProScan

Transcript Database ID Name Start End E.value
16 g4831.t1 Gene3D G3DSA:1.10.630.10 Cytochrome p450 31 490 1.4E-107
2 g4831.t1 PANTHER PTHR24291:SF143 CYTOCHROME P450 4D1-RELATED 8 480 5.0E-132
3 g4831.t1 PANTHER PTHR24291 CYTOCHROME P450 FAMILY 4 8 480 5.0E-132
6 g4831.t1 PRINTS PR00463 E-class P450 group I signature 180 198 1.4E-25
9 g4831.t1 PRINTS PR00463 E-class P450 group I signature 300 317 1.4E-25
11 g4831.t1 PRINTS PR00385 P450 superfamily signature 311 328 5.3E-12
5 g4831.t1 PRINTS PR00463 E-class P450 group I signature 320 346 1.4E-25
4 g4831.t1 PRINTS PR00463 E-class P450 group I signature 362 380 1.4E-25
14 g4831.t1 PRINTS PR00385 P450 superfamily signature 363 374 5.3E-12
10 g4831.t1 PRINTS PR00463 E-class P450 group I signature 402 426 1.4E-25
8 g4831.t1 PRINTS PR00463 E-class P450 group I signature 433 443 1.4E-25
12 g4831.t1 PRINTS PR00385 P450 superfamily signature 434 443 5.3E-12
7 g4831.t1 PRINTS PR00463 E-class P450 group I signature 443 466 1.4E-25
13 g4831.t1 PRINTS PR00385 P450 superfamily signature 443 454 5.3E-12
1 g4831.t1 Pfam PF00067 Cytochrome P450 34 475 3.1E-96
18 g4831.t1 Phobius SIGNAL_PEPTIDE Signal peptide region 1 21 -
19 g4831.t1 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 5 -
20 g4831.t1 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 6 16 -
21 g4831.t1 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 17 21 -
17 g4831.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 22 493 -
23 g4831.t1 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 436 445 -
15 g4831.t1 SUPERFAMILY SSF48264 Cytochrome P450 34 483 6.94E-103
22 g4831.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 2 21 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed