Gene loci information

Transcript annotation

  • This transcript has been annotated as Cytochrome P450 6A1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g4849 g4849.t1 isoform g4849.t1 5395352 5397116
chr_2 g4849 g4849.t1 exon g4849.t1.exon1 5395352 5395508
chr_2 g4849 g4849.t1 cds g4849.t1.CDS1 5395352 5395508
chr_2 g4849 g4849.t1 exon g4849.t1.exon2 5395573 5395831
chr_2 g4849 g4849.t1 cds g4849.t1.CDS2 5395573 5395831
chr_2 g4849 g4849.t1 exon g4849.t1.exon3 5395892 5396065
chr_2 g4849 g4849.t1 cds g4849.t1.CDS3 5395892 5396065
chr_2 g4849 g4849.t1 exon g4849.t1.exon4 5396189 5397116
chr_2 g4849 g4849.t1 cds g4849.t1.CDS4 5396189 5397116
chr_2 g4849 g4849.t1 TSS g4849.t1 NA NA
chr_2 g4849 g4849.t1 TTS g4849.t1 NA NA

Sequences

>g4849.t1 Gene=g4849 Length=1518
ATGATTGTAAATATTTTACTTTATGTTGTTTTGCCAATTGCAACGGCAATTTACATTTAC
TTCAATAGAAAGTTCAATCATTTCAAAGAACGTGGCATTCCTCATTTTAAGCCATCATTT
CCACTTGGAAATATGCAAGGAATCGGCACAAAATATCATTCAATTGACATTATTAAAAAT
GTTTATGATGAATTTAAAGGAAAAGATGTAATTGCTGGACTTTATAATTTGAATCAAGCT
TTCTATGTGGTCACAGATGCTGAACTTGTCAAGAATATTTTAGTTCGTGATTTCAATAAT
TTTGTCAATCGAGGAATGTATGTAAATGAGGAAGGTGAACCTTTGACTGGTCATTTGTTT
GCAGTGAGAGATGAAAAGTGGAAATTTCTAAGAAATAAGCTTTCGCCGGCTTTTACTTCA
GGAAAGATGAAGGCCATGTATTACACAATTAGTGACAAAGGAAAAGATTATGTTTCATTA
TTTGATGCTGCTTTAGAAAAGAGCAAAACTCTTGATATCAAAAATTTTACCAGTCGTTAT
ACAATTGATGTTGTTTCTTCAGTTGCTTTTGGAATGGAATCAAATACATTAAAAGGACAA
AATGATGAACTACTTGTGATTTTTAAAGAACTTTTTGGTGAAGAAGCAAGTCGCAATTTT
TTCAAATTTTTCTTCATGTCAGCTTTTCCTGAAATTTCTAAAAAGCTACATTTGAAATTA
TTTTCTCAAAAAATTTCTCAATATTTTATAAATGTCATTGGTAAAAATATTGAAAATCGT
GAAATAACAAATGACAATCGACCAGATTTCTTGAACATGTTGATTCAGTTGAAGAACAAA
GGATCAATTGATGGAGAATTTTCAAAAGAGAACAAAAAAATTACTTTGAATGAAGCAATG
GCACAAACTTTTGTCTTCTTTTTTGCAGGCGCAGACAGTTCAAGTACAGCGATTGCTTTT
GCATTAGCTGAACTCGCTCACAATACAGAAGCACAAAATAAATTAAGAGCTGAAATTTTG
GAAAAGACTAAAGGAAAAGAAAATGAAGATATAAATTATGAAACATTGCAAGAAATGACT
TATTTGAATCAAGTTGTTAATGAAACCCTTCGAATGTACACAATTAATTCATTATTATTG
CGTCAAGCAAATGAAGATTACAAAATTCCAAACTCAAATCATGTTATTCCAAAAAAGGCT
AATATTATTATTCCTATGGTAGGATTTCACTATGATGACAGATATTGGAAGAATCCTGAA
AAATTCGATCCTGAAAGATTTACACCAGAAGAAATCGCAAAAAGACCAAACCATGTTTAT
CTTCCATTTGGTGATGGAATGAGAAACTGTATCGGAATGAGATATGGGCTTTTGATAGTC
AAATTTGCAATTGCAACAACATTGAGAAATTACAAACTTAAACTGAATCCAAAAATGAAA
TATCCGATTAAACTTGATCCGAAAAATCCTCTTTTGGAGCCAGAAGGTGGTTTTCATTTG
GACCTTGAAAGAATTTAG

>g4849.t1 Gene=g4849 Length=505
MIVNILLYVVLPIATAIYIYFNRKFNHFKERGIPHFKPSFPLGNMQGIGTKYHSIDIIKN
VYDEFKGKDVIAGLYNLNQAFYVVTDAELVKNILVRDFNNFVNRGMYVNEEGEPLTGHLF
AVRDEKWKFLRNKLSPAFTSGKMKAMYYTISDKGKDYVSLFDAALEKSKTLDIKNFTSRY
TIDVVSSVAFGMESNTLKGQNDELLVIFKELFGEEASRNFFKFFFMSAFPEISKKLHLKL
FSQKISQYFINVIGKNIENREITNDNRPDFLNMLIQLKNKGSIDGEFSKENKKITLNEAM
AQTFVFFFAGADSSSTAIAFALAELAHNTEAQNKLRAEILEKTKGKENEDINYETLQEMT
YLNQVVNETLRMYTINSLLLRQANEDYKIPNSNHVIPKKANIIIPMVGFHYDDRYWKNPE
KFDPERFTPEEIAKRPNHVYLPFGDGMRNCIGMRYGLLIVKFAIATTLRNYKLKLNPKMK
YPIKLDPKNPLLEPEGGFHLDLERI

Protein features from InterProScan

Transcript Database ID Name Start End E.value
16 g4849.t1 Gene3D G3DSA:1.10.630.10 Cytochrome p450 16 505 2.0E-123
2 g4849.t1 PANTHER PTHR24292 CYTOCHROME P450 6 504 5.2E-159
3 g4849.t1 PANTHER PTHR24292:SF95 CYP6A16, ISOFORM B-RELATED 6 504 5.2E-159
8 g4849.t1 PRINTS PR00463 E-class P450 group I signature 89 110 4.1E-17
6 g4849.t1 PRINTS PR00463 E-class P450 group I signature 298 315 4.1E-17
12 g4849.t1 PRINTS PR00385 P450 superfamily signature 309 326 1.2E-9
5 g4849.t1 PRINTS PR00463 E-class P450 group I signature 318 344 4.1E-17
4 g4849.t1 PRINTS PR00463 E-class P450 group I signature 363 381 4.1E-17
14 g4849.t1 PRINTS PR00385 P450 superfamily signature 364 375 1.2E-9
10 g4849.t1 PRINTS PR00463 E-class P450 group I signature 405 429 4.1E-17
7 g4849.t1 PRINTS PR00463 E-class P450 group I signature 440 450 4.1E-17
11 g4849.t1 PRINTS PR00385 P450 superfamily signature 441 450 1.2E-9
9 g4849.t1 PRINTS PR00463 E-class P450 group I signature 450 473 4.1E-17
13 g4849.t1 PRINTS PR00385 P450 superfamily signature 450 461 1.2E-9
1 g4849.t1 Pfam PF00067 Cytochrome P450 42 495 9.0E-91
18 g4849.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 5 -
19 g4849.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 6 21 -
17 g4849.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 22 505 -
21 g4849.t1 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 443 452 -
15 g4849.t1 SUPERFAMILY SSF48264 Cytochrome P450 33 503 1.83E-103
20 g4849.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 2 21 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed