General information for g4853.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative fatty acyl-CoA reductase CG5065.
Parent gene
- The parent gene g4853 is located on chr_2: 5419745..5421767 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g4853 | g4853.t1 | isoform | g4853.t1 | 5419745 | 5421767 |
| chr_2 | g4853 | g4853.t1 | exon | g4853.t1.exon1 | 5419745 | 5420102 |
| chr_2 | g4853 | g4853.t1 | cds | g4853.t1.CDS1 | 5419745 | 5420102 |
| chr_2 | g4853 | g4853.t1 | exon | g4853.t1.exon2 | 5420284 | 5420526 |
| chr_2 | g4853 | g4853.t1 | cds | g4853.t1.CDS2 | 5420284 | 5420526 |
| chr_2 | g4853 | g4853.t1 | exon | g4853.t1.exon3 | 5420738 | 5420898 |
| chr_2 | g4853 | g4853.t1 | cds | g4853.t1.CDS3 | 5420738 | 5420898 |
| chr_2 | g4853 | g4853.t1 | exon | g4853.t1.exon4 | 5420952 | 5421767 |
| chr_2 | g4853 | g4853.t1 | cds | g4853.t1.CDS4 | 5420952 | 5421767 |
| chr_2 | g4853 | g4853.t1 | TSS | g4853.t1 | 5421823 | 5421823 |
| chr_2 | g4853 | g4853.t1 | TTS | g4853.t1 | NA | NA |
Sequences
>g4853.t1 Gene=g4853 Length=1578
ATGAAGTTTCCAAATGGATCATTCAGTCCTTTTTTATCGCATGATGATTGGATTAAAAAG
AATTTGAAGAAACCTACACCATTGAGTCATTTACCATCAATTCCTGAATTTTACGCTAAT
AAGGAAATTTTTGTGACTGGATTTTCTGGATTTATTGGAAAAGTTTTGATCGAGAAATTA
CTGAGATCGTGTCCAAAAATTAAAGCAATTTATGTGTTATTAAGAGCTAAGAAAGGTCAA
ACAATTGAAGAAAGATTGAAGACTTTAACTAAACAAGTTGTTTTCGACCTTCTACGAAAA
GAAAATCCAAATTTCGCTGAAAAAATTATTCCAATTGAAGGCGATGTCATGGAATTGAAT
TTGGGAATATCAAATAAAAATTTTGAGAAGATAAAAAATGTCTCAATTGTTTTTCATTCA
GCTGCAAGTATTCGTTTTGATGACAATTTAAAATTTGCAGTTTTAACTAACACTAGAGGA
ACAAGAGAAGTCATGAGACTTGCAGAAAAGATGAAAAATATTAAAGTTGTTATGCATGTG
TCTACAACTTATAGCAATGTTTATTTAGAAACTGTTGAAGAAAAAATTTATCCACCTTTG
GTTGATTGGGAGAAAACAATTAAAATTTGTGAAGAATCTGATCAAAATCAAGTTGATATT
TTGACTCATCATTATATCAATTTTATGCCAAATACGTATGTTTTTTCAAAAAATCTAGCA
GAGCATTGTTCTTATAGTTTTCATGATCAACTGCCAATTATCATTTTTCGACCTTCTATT
GTCGTATCATCTCATGTCGAACCATTTGCTGGATATGTTGATAATTTTAATGGACCAATG
GGTATTCTTTTAGCATCAGCAGTTGGAATCACTAAAACAATGTATTGTGATGAAAAAAAT
GTACTGGATTGTTTGCCAGTTGATGTTTGTGTTAAAGCTATGATTGTATCTGCTTGGAAG
AGAGCTCATGAGATTGACAACACACCCCCAGTTATGAATGCAGCTTGTGCAAATCAATTT
AAAGTTACAATAAAGCAATTTCTTGATATGGGAATACATGGAATTTGTGAAGAATTTCCA
ATGGGTAAAAAGATGGTTTTTCTACCATTTGGAGGAGTAACTTTATGTAAAACTTGGAAT
TTCATTCGATTGATATTTATGCAATTATCACTTGCTGTTTTGGTTGATGCTATTTTAAAA
GTTAAAGGAAAAAGACCAAGAATTATGAAACTTCAAAGAAAAATATATGAAGCTAACAAA
GCTCTTGGACATTTTGTAACACATAATTGGAATTTTAAAAATGAAAGTTTCATCAATTTG
TGCACTTATTTGAAACCTGAAGATGTTTCATCATTTGATTATCGACCATTCTTTACTACA
GATGTTATAAATTTTGGAAGATATGTTTGTTATGGATTTCGAAGATATTTATTGAAATTG
AAAGATGAAGATTTAGAAAAAGATCGAAAAAATGTCAAGTTAATGCTTTTTGCTGTAACA
ATTTTTAAATGTCTTGGTGCTTTCATAATTTTTGGTTTGATTTTCTTTAAATACAATGGA
AATTATTTTTTTCAATAA
>g4853.t1 Gene=g4853 Length=525
MKFPNGSFSPFLSHDDWIKKNLKKPTPLSHLPSIPEFYANKEIFVTGFSGFIGKVLIEKL
LRSCPKIKAIYVLLRAKKGQTIEERLKTLTKQVVFDLLRKENPNFAEKIIPIEGDVMELN
LGISNKNFEKIKNVSIVFHSAASIRFDDNLKFAVLTNTRGTREVMRLAEKMKNIKVVMHV
STTYSNVYLETVEEKIYPPLVDWEKTIKICEESDQNQVDILTHHYINFMPNTYVFSKNLA
EHCSYSFHDQLPIIIFRPSIVVSSHVEPFAGYVDNFNGPMGILLASAVGITKTMYCDEKN
VLDCLPVDVCVKAMIVSAWKRAHEIDNTPPVMNAACANQFKVTIKQFLDMGIHGICEEFP
MGKKMVFLPFGGVTLCKTWNFIRLIFMQLSLAVLVDAILKVKGKRPRIMKLQRKIYEANK
ALGHFVTHNWNFKNESFINLCTYLKPEDVSSFDYRPFFTTDVINFGRYVCYGFRRYLLKL
KDEDLEKDRKNVKLMLFAVTIFKCLGAFIIFGLIFFKYNGNYFFQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g4853.t1 | CDD | cd05236 | FAR-N_SDR_e | 41 | 350 | 1.26647E-117 |
| 15 | g4853.t1 | CDD | cd09071 | FAR_C | 386 | 478 | 2.08596E-17 |
| 8 | g4853.t1 | Gene3D | G3DSA:3.40.50.720 | - | 15 | 360 | 3.5E-68 |
| 3 | g4853.t1 | PANTHER | PTHR11011:SF24 | FATTY ACYL-COA REDUCTASE | 31 | 517 | 2.2E-173 |
| 4 | g4853.t1 | PANTHER | PTHR11011 | MALE STERILITY PROTEIN 2-RELATED | 31 | 517 | 2.2E-173 |
| 1 | g4853.t1 | Pfam | PF07993 | Male sterility protein | 45 | 314 | 2.0E-71 |
| 2 | g4853.t1 | Pfam | PF03015 | Male sterility protein | 390 | 479 | 3.9E-17 |
| 10 | g4853.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 380 | - |
| 13 | g4853.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 381 | 399 | - |
| 9 | g4853.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 400 | 493 | - |
| 12 | g4853.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 494 | 516 | - |
| 11 | g4853.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 517 | 525 | - |
| 7 | g4853.t1 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 33 | 327 | 8.01E-29 |
| 6 | g4853.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 379 | 401 | - |
| 5 | g4853.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 494 | 516 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0080019 | fatty-acyl-CoA reductase (alcohol-forming) activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed