Gene loci information

Transcript annotation

  • This transcript has been annotated as Probable cytochrome P450 12a5, mitochondrial.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g4970 g4970.t1 TSS g4970.t1 6210086 6210086
chr_2 g4970 g4970.t1 isoform g4970.t1 6210139 6212287
chr_2 g4970 g4970.t1 exon g4970.t1.exon1 6210139 6210362
chr_2 g4970 g4970.t1 cds g4970.t1.CDS1 6210139 6210362
chr_2 g4970 g4970.t1 exon g4970.t1.exon2 6210429 6210492
chr_2 g4970 g4970.t1 cds g4970.t1.CDS2 6210429 6210492
chr_2 g4970 g4970.t1 exon g4970.t1.exon3 6210552 6210781
chr_2 g4970 g4970.t1 cds g4970.t1.CDS3 6210552 6210781
chr_2 g4970 g4970.t1 exon g4970.t1.exon4 6211044 6211319
chr_2 g4970 g4970.t1 cds g4970.t1.CDS4 6211044 6211319
chr_2 g4970 g4970.t1 exon g4970.t1.exon5 6211379 6211521
chr_2 g4970 g4970.t1 cds g4970.t1.CDS5 6211379 6211521
chr_2 g4970 g4970.t1 exon g4970.t1.exon6 6211583 6211596
chr_2 g4970 g4970.t1 cds g4970.t1.CDS6 6211583 6211596
chr_2 g4970 g4970.t1 exon g4970.t1.exon7 6211656 6212071
chr_2 g4970 g4970.t1 cds g4970.t1.CDS7 6211656 6212071
chr_2 g4970 g4970.t1 exon g4970.t1.exon8 6212125 6212287
chr_2 g4970 g4970.t1 cds g4970.t1.CDS8 6212125 6212287
chr_2 g4970 g4970.t1 TTS g4970.t1 6212308 6212308

Sequences

>g4970.t1 Gene=g4970 Length=1530
ATGTTGATCCGCCAGACTGCTTCTATTTCTCGACGTCTTTTGACGACTACATCCACGTCA
TTCAGCCTTGACAATCAATTGAGTCAAAATAAGTCATTCTCTGAAATACCTTCTATTTCA
GCTCTTAAAGTTGTGGTTCGAAATATACCAGGTGGAAAATACTATAATAAATCTATGACA
CAGCTTCATGAGCTTTTATATGATGAATATGGATCTATATTGAGATTTCCTGGATTATTT
GGTCGACCACCAGTAGTTTTTGCATATGACGCAAAAGATTTTGAAAAAGTATATAGGAAT
GAAGGTGTATGGCCAGAGAGATTAAACTTGGATTCATTAGTTTATTATAGAAAAACAAAG
CGTTCTGACATTTATGATAAATTTGCTGGTTTATTTTCTTCTCAAGGTGAAGAATGGCAC
AGTATGAGAACAATTACCAATCCGATTTTGATGCAACCAAAAACTATGAAACTTTATTGC
ACACAAGTTGATGAAATAGCGAAGGAATTTGTCGGAATCATGCGGAAATTAAGAGATGAG
AAATGTGAAATGCCTGCTGATTTTAGCAATTACATAAATTCATGGTCGTTAGAGTCAGTT
GTTGCTGTTGCTCTAGAAAGACGTTTAAATATATTGAGTGGGTCTTCTAATGATGAAAAA
GCTCAAGAGCTCATCAAAAATATTAGAATATTTTTTGAAAAATCTTTTGAATTTGATGGA
CTTCCTTCAGTTTGGCGTTACTATGAAACAAAAAGTTTCAAAGATTTCCTAAAAGTTTAT
GATACGATAACTAATATTGTGCTGTATTTTATTGAAGAAGCAGTAGCAAGAATAGAATCA
AATCCTTCTTTATCATCTAACGAAGATGGTATACTGAGAAAACTTTTAAAAATTGATAAA
AAAGCTGCTGTTGTTATGGCAAGCGACATGCTATTAGCTGGTGTTGACACAACAGCAACA
GCAGCTATAAACACGCTTTACTGCCTCGCAAAAAATCCTGACAAACAGGAAATTCTTCGA
AAGGAATTGATAAACATTTTGCAAACGAAGGAAACACAATTGACTGCCGAAAATATGAAA
AATTTACCATATTTAAGAGGTGTAATCAAAGAAACATTGAGAATTATGCCAGTCATTAGT
GGGACAGCTAGAAAGGTATCAAATGATGTCAGTATATCGGGATATAAAGTACCTGCTGGT
ACACATGTGATCATGGTGCCTATATTTGAAACTACAAATGAAAAGCAATATCCACAGCCT
GAAAAGTTTATGCCTGAACGATGGCTCCGTGAAAATAACGACCCACAATGTCCTCATGCT
AAAAATGCCAATCCATTTACATTTCTTCCATTTGGTTTTGGAAGCAGAATGTGCATTGGA
CGTCGTTTGGCTGAACTGGAAATTGAAGTGTTGATTGCAAATGTCATCAGAAATTTTAAG
CTTGAATGGCATCATCCTGATATGAAAATTAAAAGTACTTTTGTAAACCTCCCAATTTCA
GATTTAAAATTTAAAGCTATTGATTTATGA

>g4970.t1 Gene=g4970 Length=509
MLIRQTASISRRLLTTTSTSFSLDNQLSQNKSFSEIPSISALKVVVRNIPGGKYYNKSMT
QLHELLYDEYGSILRFPGLFGRPPVVFAYDAKDFEKVYRNEGVWPERLNLDSLVYYRKTK
RSDIYDKFAGLFSSQGEEWHSMRTITNPILMQPKTMKLYCTQVDEIAKEFVGIMRKLRDE
KCEMPADFSNYINSWSLESVVAVALERRLNILSGSSNDEKAQELIKNIRIFFEKSFEFDG
LPSVWRYYETKSFKDFLKVYDTITNIVLYFIEEAVARIESNPSLSSNEDGILRKLLKIDK
KAAVVMASDMLLAGVDTTATAAINTLYCLAKNPDKQEILRKELINILQTKETQLTAENMK
NLPYLRGVIKETLRIMPVISGTARKVSNDVSISGYKVPAGTHVIMVPIFETTNEKQYPQP
EKFMPERWLRENNDPQCPHAKNANPFTFLPFGFGSRMCIGRRLAELEIEVLIANVIRNFK
LEWHHPDMKIKSTFVNLPISDLKFKAIDL

Protein features from InterProScan

Transcript Database ID Name Start End E.value
16 g4970.t1 Gene3D G3DSA:1.10.630.10 Cytochrome p450 24 507 3.0E-119
2 g4970.t1 PANTHER PTHR24291:SF155 CYTOCHROME P450 301B1 30 495 4.3E-118
3 g4970.t1 PANTHER PTHR24291 CYTOCHROME P450 FAMILY 4 30 495 4.3E-118
9 g4970.t1 PRINTS PR00463 E-class P450 group I signature 302 319 4.2E-17
11 g4970.t1 PRINTS PR00385 P450 superfamily signature 313 330 8.0E-12
8 g4970.t1 PRINTS PR00463 E-class P450 group I signature 322 348 4.2E-17
4 g4970.t1 PRINTS PR00463 E-class P450 group I signature 366 384 4.2E-17
10 g4970.t1 PRINTS PR00385 P450 superfamily signature 367 378 8.0E-12
6 g4970.t1 PRINTS PR00463 E-class P450 group I signature 406 430 4.2E-17
5 g4970.t1 PRINTS PR00463 E-class P450 group I signature 448 458 4.2E-17
12 g4970.t1 PRINTS PR00385 P450 superfamily signature 449 458 8.0E-12
7 g4970.t1 PRINTS PR00463 E-class P450 group I signature 458 481 4.2E-17
13 g4970.t1 PRINTS PR00385 P450 superfamily signature 458 469 8.0E-12
1 g4970.t1 Pfam PF00067 Cytochrome P450 54 490 2.1E-80
15 g4970.t1 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 451 460 -
14 g4970.t1 SUPERFAMILY SSF48264 Cytochrome P450 50 506 3.41E-87

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

Pathway

This gene does not belong to any pathways.

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values