Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Chitooligosaccharidolytic beta-N-acetylglucosaminidase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g5193 g5193.t3 isoform g5193.t3 7603757 7605657
chr_2 g5193 g5193.t3 exon g5193.t3.exon1 7603757 7605562
chr_2 g5193 g5193.t3 cds g5193.t3.CDS1 7604064 7604723
chr_2 g5193 g5193.t3 exon g5193.t3.exon2 7605638 7605657
chr_2 g5193 g5193.t3 TSS g5193.t3 NA NA
chr_2 g5193 g5193.t3 TTS g5193.t3 NA NA

Sequences

>g5193.t3 Gene=g5193 Length=1826
TGCATTTTCTAAAGATAAAGTTTATTTTAAATCTGATATTGATAAAATTGTTCAATATGC
AAAATATCGTGGTATTCGAGTGATTTTTGAGCTTGATGCGCCTGCTCATGCGGGAAAAGG
TTGGGAATGGGGTGAAAAATCTGGCTTAGGAAAACTTGCAGTTTGTGTTGATGATCAGCC
ATGGAGAAAATCGTGCATTCAACCAAATTGTGGACAATTAAATCCATCAAATGAAAACCT
TTATTCAGTATTGCATGACATTTATCAAGATATTCGTGATTATAAAGAGAAAAATGAATT
TATACACATGGGTGGCGATGAAGTCTTTGTCAAGTGTTGGAATAATACAAAAGAAATCAC
AGACTATATGGCAACAAAAAATTATGATCGCAATAATGTCATTGGCTTTTTACAGTTATG
GTCTGAATTTCAAGGAAAAGCTTTAAATATTTGGGATAACATCACAGATGAAAAAGAATC
AATTGTAATGTGGTCAAGTGAATTGACACTGCCTGAAAATATTGAAAAATTCTTACCTAA
AGAACGTTATATCATTCAAACTTGGATTCCAAATAATTCTGATATTCCTCAAAAGTTGTT
AGATAAAGGCTATCGACTCATAATGACTACAAAAAATGCTTGGTATTTTGACCATGGTTT
TTGGGGCAATACAAAATATTATAATTGGAAAACAGTTTATGCAAATACTCTTCCACGAAA
TGAGTTGGTTTTAGGTGGTGAAGTTTGCATGTGGTCAGAATATTTGGATGAACATTCAAT
TGAAATGAAAATTTTTCCACGTCTTAATGCTGCTGCAGAACGTTTGTGGGCAAATCCAAA
AACTGATCTTAATGCTGTAGAATCCAGATTTTATCGTCAACGTGAGAGAATCGTTGCAAA
AGGAATTCAAGCTGATGCTATTTTAACTGAATATTGTACATTGTTTGAAGGTGAATGCCG
TGGTTAATGTGTGAAAAATGAACACATTTATTTATTTTAATAATTTACATTACAATTAAT
TAAGTTGCACATTTAATAAAAGCATCAGTATTAGATAAATTTGCACACTTATTTGCTCTT
TTGGCTCTCTGCTGCATTCCAGTAAATTTCAGGTAATGTGCTCTTTGCAAATGGCCATAA
AATGCATGACAGCCAATTGTCGCCAAAAACAATCTTCAAATTTTTATTAAGGCCGAGATC
ATATGCGCCTTTGTTTTTCTCATGTGCTGTTGCATTTTTAACTATAAGACGTCCATGATA
AACCATCAAAACTCCAGTAAAAATTGCTCCAATTAAGATCAGCATGTATAATAAAAGATG
AATTTCATTTGATGTATTAAACATGAACATAAACATAGGAAAAATCATTTTCAAAGGTGT
AATCCATTGAAGAAAAAGTGAACCATTAACAATCCACAAATAGTATGAATTATAAAAGAA
TGCATAAGTTGTTCCAATAAAGAAGTAAAGTAAAAACACCAAAAAATGTCGATGATTATT
ATAGCCAATACAATTTCCAGCAAACATACAGTGATGATCACGTTTTAGAATGCAGACATT
ACATGTATCACAATGCCAGCTTCGAGGCGGTACAATTATTTCGCATTTTTCACACAAACT
CCAATCTTTTGCTGATGGATCATTTTTGAAGCGAGTTGTTGTCATTGTTTCAGGAGAAGC
AAAAACACTTGTGTCTATTAAAATTATGTGCATCAAATTTGCGATTATGTTAAATACGAT
GAATGTACCAAGTATATAGTTGACTAGCAGAATAATTGAAAATCCATGAATGTGATAAAG
AACATAATGAAGTTCATAGTAAAACA

>g5193.t3 Gene=g5193 Length=219
MGGDEVFVKCWNNTKEITDYMATKNYDRNNVIGFLQLWSEFQGKALNIWDNITDEKESIV
MWSSELTLPENIEKFLPKERYIIQTWIPNNSDIPQKLLDKGYRLIMTTKNAWYFDHGFWG
NTKYYNWKTVYANTLPRNELVLGGEVCMWSEYLDEHSIEMKIFPRLNAAAERLWANPKTD
LNAVESRFYRQRERIVAKGIQADAILTEYCTLFEGECRG

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g5193.t3 Gene3D G3DSA:3.20.20.80 Glycosidases 1 219 0e+00
2 g5193.t3 PANTHER PTHR22600 BETA-HEXOSAMINIDASE 1 217 0e+00
3 g5193.t3 PANTHER PTHR22600:SF42 BETA-HEXOSAMINIDASE 1 217 0e+00
4 g5193.t3 PRINTS PR00738 Glycosyl hydrolase family 20 signature 141 157 2e-06
5 g5193.t3 PRINTS PR00738 Glycosyl hydrolase family 20 signature 158 175 2e-06
1 g5193.t3 Pfam PF00728 Glycosyl hydrolase family 20, catalytic domain 1 176 0e+00
6 g5193.t3 SUPERFAMILY SSF51445 (Trans)glycosidases 1 178 0e+00

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

Data is missing for g5193/g5193.t3; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5193.t3.fa.iupred3.txt does not exist

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004563 beta-N-acetylhexosaminidase activity MF
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds MF
GO:0005975 carbohydrate metabolic process BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values