General information for g5297.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Pancreatic triacylglycerol lipase .
Parent gene
- The parent gene g5297 is located on chr_2: 8553644..8554711 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5297 | g5297.t1 | TTS | g5297.t1 | 8553495 | 8553495 |
| chr_2 | g5297 | g5297.t1 | isoform | g5297.t1 | 8553644 | 8554711 |
| chr_2 | g5297 | g5297.t1 | exon | g5297.t1.exon1 | 8553644 | 8553930 |
| chr_2 | g5297 | g5297.t1 | cds | g5297.t1.CDS1 | 8553644 | 8553930 |
| chr_2 | g5297 | g5297.t1 | exon | g5297.t1.exon2 | 8553985 | 8554711 |
| chr_2 | g5297 | g5297.t1 | cds | g5297.t1.CDS2 | 8553985 | 8554711 |
| chr_2 | g5297 | g5297.t1 | TSS | g5297.t1 | 8554744 | 8554744 |
Sequences
>g5297.t1 Gene=g5297 Length=1014
ATGAATAGTTTCATTTATTTCTCAGTTTTACTTGCATTTGTCAACTCAGCTTCGGCTGGT
TGTATGGATAATGTTAATTTAAAATTCTTCGGCAGTTCTTACACTGATTTTCAATCTGTA
TCAATGCCTGAGCCAATTAATGTCTTATCTTACATTACTTTCTGGAGTATTCCATCTTTG
CCATCATCAACTCAATATTATGATCCAAATAAGAAAACTGTGATCTACGTACATGGACTA
TTTGAAGGAACTGACACAGTTGGACCAAAAGCAATGATCGAAGGTTATTTGCAAAGAAAA
AGTGAATACAATGTAATTCTTTATGACTACAGCTCATGTGGTGAGAAATTTTTGACATTT
ACAACACATCAAGTTGATTTACTTGAAGGATCAAATGCACTTGCAAAAATGAATTTTGAT
TTTACAAAAGTTCACATAATTGGTTATGGGTATGGAACGTTTATAGCAGCAAATGTTGGA
AGAAAACTCAATGGAAATTTAAGAAGAATCACTGGTTTAGAACCAGCAGGAATTTTGGAT
AATATTTTGGGACCACTTGGATATGCACAACTTAATAAAGATAATGCACAATTTGTTGAT
GTCATTCATACAGATACAGGATTTCTTGGTGATAAAAAGAGCCTTGGTCATGTTGATTTT
TGGGTTAATGGTGGTTGGCATCAACCTTCATGTCTTCAAAGTGTTTTTGATATCAATGAA
TTTCTTCAAAATTCAACGTTAGTCATTGCAGTTGTTTGTAATGATGCAAGATCATGGATG
TATTATGCAGAATCTGTTAATAGTCCAGCATCACCACTTCCTTTTCAATCTTTAAAATGT
GCAACCAATTATGCAACTGCAACTGAAAATTCTTATACAGCTAGCAATTGCAATGGTTCA
CCAATAGTGTCAATGGGCTATTATGCTGATTTGAGTAAAAATGATTTAATTCAATCAACA
TCCAACAATTACTTTGTTGCAACTAATGGCGCCTCGAAATTTTCTTTGACATAA
>g5297.t1 Gene=g5297 Length=337
MNSFIYFSVLLAFVNSASAGCMDNVNLKFFGSSYTDFQSVSMPEPINVLSYITFWSIPSL
PSSTQYYDPNKKTVIYVHGLFEGTDTVGPKAMIEGYLQRKSEYNVILYDYSSCGEKFLTF
TTHQVDLLEGSNALAKMNFDFTKVHIIGYGYGTFIAANVGRKLNGNLRRITGLEPAGILD
NILGPLGYAQLNKDNAQFVDVIHTDTGFLGDKKSLGHVDFWVNGGWHQPSCLQSVFDINE
FLQNSTLVIAVVCNDARSWMYYAESVNSPASPLPFQSLKCATNYATATENSYTASNCNGS
PIVSMGYYADLSKNDLIQSTSNNYFVATNGASKFSLT
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g5297.t1 | Gene3D | G3DSA:3.40.50.1820 | - | 40 | 335 | 7.0E-56 |
| 2 | g5297.t1 | PANTHER | PTHR11610 | LIPASE | 52 | 334 | 9.6E-46 |
| 5 | g5297.t1 | PRINTS | PR00821 | Triacylglycerol lipase family signature | 61 | 80 | 2.8E-6 |
| 3 | g5297.t1 | PRINTS | PR00821 | Triacylglycerol lipase family signature | 143 | 161 | 2.8E-6 |
| 4 | g5297.t1 | PRINTS | PR00821 | Triacylglycerol lipase family signature | 253 | 268 | 2.8E-6 |
| 1 | g5297.t1 | Pfam | PF00151 | Lipase | 68 | 334 | 7.1E-40 |
| 11 | g5297.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 19 | - |
| 12 | g5297.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 13 | g5297.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 14 | - |
| 14 | g5297.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 19 | - |
| 10 | g5297.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 20 | 337 | - |
| 6 | g5297.t1 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 60 | 334 | 2.11E-39 |
| 8 | g5297.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 19 | - |
| 15 | g5297.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 19 | - |
| 7 | g5297.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 19 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5297/g5297.t1; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5297.t1.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004806 | triglyceride lipase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed