General information for g5491.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Enoyl-CoA delta isomerase 1, mitochondrial.
Parent gene
- The parent gene g5491 is located on chr_2: 9849639..9850656 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5491 | g5491.t1 | isoform | g5491.t1 | 9849639 | 9850656 |
| chr_2 | g5491 | g5491.t1 | exon | g5491.t1.exon1 | 9849639 | 9850316 |
| chr_2 | g5491 | g5491.t1 | cds | g5491.t1.CDS1 | 9849639 | 9850316 |
| chr_2 | g5491 | g5491.t1 | exon | g5491.t1.exon2 | 9850384 | 9850656 |
| chr_2 | g5491 | g5491.t1 | cds | g5491.t1.CDS2 | 9850384 | 9850656 |
| chr_2 | g5491 | g5491.t1 | TSS | g5491.t1 | NA | NA |
| chr_2 | g5491 | g5491.t1 | TTS | g5491.t1 | NA | NA |
Sequences
>g5491.t1 Gene=g5491 Length=951
ATGCTTTCATCTAAATTTATACACGCATTTACACGTCAAATTTATCACACTAATTTAATA
ACAAGTGCCACATTTAATGTAATAAAAAAACATAATTCTACACATACGAAGAATGATTTA
GTTTTAGTGGATGTAAATGACAAAACAGGATTTTCTATTGTCACATTAAATAGGCCTCCT
GTGAATTCACTCAATTTAGAACTCTTATCAGCATTTTCATCAGTTTTAGATGATTTACAT
AAAAATAATTCACGAGGAATGATCCTCACTAGTTCTTCTTCAACAGTTTTCTCAGCTGGT
CTCGATATAAATGAATTGTACAAGCCTGATATGGATCGAGTTCGTAAATTTTGGACCTCT
TTACAAGATTGTTGGATAAAACTTTATGGATCATCCTTTCCAACTGCTGCTGCTATAAAT
GGACATGCACCTGCTGGTGGATGTTTATTTGCAATGTGCTGCGAATATCGAGTGATGCTT
CCTAAATATACGATAGGATTAAATGAAACTCAACTTGGAATTGTTGCTCCAACATGGTTT
ATGGCGACTATGAAAAATACAATACCATTGAGACAAGCAGAACTTGCATTAACACTTGGA
AAAATGTTTAAAACAGATGATGCATTAAAATTAGGCCTTATCGATGAAATAGCTGAAAAT
AAAGAGGATGCAATTCATAAATGCGAAATATTTCTTAAGAAATATCAGAAAATTCCACAA
ACGGCACGAGCATTGACAAAACAAAGTTATAGAAAAAATGATATTATGGAACTTGAAAAT
AACAGAGAGGCTGATCTTCAATTGTTCTTTCTTGCAATATCAGATCCAAGAGTTCAAAGT
TCTTTAGGCAAATATGTTGCATTTCTTAAATTGAAATCAAGAATGAAACCAATACTTGAA
GCAGTTAAATTTCTAAAAGGAATATTCAAATCAAAAAAGAAGAAGAATTAA
>g5491.t1 Gene=g5491 Length=316
MLSSKFIHAFTRQIYHTNLITSATFNVIKKHNSTHTKNDLVLVDVNDKTGFSIVTLNRPP
VNSLNLELLSAFSSVLDDLHKNNSRGMILTSSSSTVFSAGLDINELYKPDMDRVRKFWTS
LQDCWIKLYGSSFPTAAAINGHAPAGGCLFAMCCEYRVMLPKYTIGLNETQLGIVAPTWF
MATMKNTIPLRQAELALTLGKMFKTDDALKLGLIDEIAENKEDAIHKCEIFLKKYQKIPQ
TARALTKQSYRKNDIMELENNREADLQLFFLAISDPRVQSSLGKYVAFLKLKSRMKPILE
AVKFLKGIFKSKKKKN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g5491.t1 | CDD | cd06558 | crotonase-like | 41 | 222 | 0 |
| 5 | g5491.t1 | Gene3D | G3DSA:3.90.226.10 | - | 38 | 268 | 0 |
| 2 | g5491.t1 | PANTHER | PTHR11941:SF45 | ENOYL-COA DELTA ISOMERASE 1, MITOCHONDRIAL | 29 | 292 | 0 |
| 3 | g5491.t1 | PANTHER | PTHR11941 | ENOYL-COA HYDRATASE-RELATED | 29 | 292 | 0 |
| 1 | g5491.t1 | Pfam | PF00378 | Enoyl-CoA hydratase/isomerase | 50 | 286 | 0 |
| 4 | g5491.t1 | SUPERFAMILY | SSF52096 | ClpP/crotonase | 38 | 280 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5491/g5491.t1; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5491.t1.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
- This transcript has been annotated with K13238 (Delta3-Delta2-enoyl-CoA isomerase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
