Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Enoyl-CoA delta isomerase 1, mitochondrial.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g5492 g5492.t1 isoform g5492.t1 9850825 9851743
chr_2 g5492 g5492.t1 exon g5492.t1.exon1 9850825 9851436
chr_2 g5492 g5492.t1 cds g5492.t1.CDS1 9850825 9851436
chr_2 g5492 g5492.t1 exon g5492.t1.exon2 9851495 9851743
chr_2 g5492 g5492.t1 cds g5492.t1.CDS2 9851495 9851743
chr_2 g5492 g5492.t1 TSS g5492.t1 9851874 9851874
chr_2 g5492 g5492.t1 TTS g5492.t1 NA NA

Sequences

>g5492.t1 Gene=g5492 Length=861
ATGTTTTTAACAAGAGTTATTAAAAATACGAATTATTTATGGAGAAGTATAGCAACAAGT
GCAGTAACAAAGTGTCAAAGTAATAGCAATCAATTGGTGATTGTTGATATCAATGATAAA
ACAGGATATGCTACTTTATCGTTGAATCGACCACCAGCAAATTCATTTAACCTTGAACAA
CTTGTGGCGTTTTCAAAAGCATTGGATGATATTGAGAAGAGTAAAGTTAAAGGAATGATT
TTAACAAGTAACGTACCAAAAATATTTTCTGTTGGATTTGATGTCAGAGAATTTAATAGC
TCTGATGAAAATCGACTTCGTGAATTTTGGACAGTTGTTCAAGATTGTTGGTTAAAGCTC
TATGGATCATATTATCCAACTGCTGCTGCCATCACTGGACATAATCCTGCTGGAGGTTGT
TTCTTTAGCATGTCGTGTGAATATCGAGTTATGGTTTCAAAATCAAAAATTGGTTTGAGT
GGAGCACGCTTAGGAATTGCAATTCCTTTACCAATTATTTTATCTATGAGAAGTATATTG
TCACCACATGCAACAGAATTAGCCATTACAACAGGAAAACTCTTTACAACTGAAGAAGCT
CTCAAAATTGGTTTGATTGATGAAATTGCAGAAGATAAAGAAGATGCTCTGCGAAAATGT
GAACGATTTCTTGATCGTTTCAAAAAAATTCCTCCAAATGCTCGTGCTTTAACAAAACAA
GCTATAAGAAAGAAGGAACTCGATGAAATTGCTAACAATCGTAAGCGTGATGTTGATGAA
TTTATAGCTGCTGTCACAAGTCATGAAGCAAAAGAATCATTTGAGATTTTTCTTGATCCA
TCAAAATACAAGGAAAATTAA

>g5492.t1 Gene=g5492 Length=286
MFLTRVIKNTNYLWRSIATSAVTKCQSNSNQLVIVDINDKTGYATLSLNRPPANSFNLEQ
LVAFSKALDDIEKSKVKGMILTSNVPKIFSVGFDVREFNSSDENRLREFWTVVQDCWLKL
YGSYYPTAAAITGHNPAGGCFFSMSCEYRVMVSKSKIGLSGARLGIAIPLPIILSMRSIL
SPHATELAITTGKLFTTEEALKIGLIDEIAEDKEDALRKCERFLDRFKKIPPNARALTKQ
AIRKKELDEIANNRKRDVDEFIAAVTSHEAKESFEIFLDPSKYKEN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g5492.t1 CDD cd06558 crotonase-like 33 226 6.55235E-42
6 g5492.t1 Coils Coil Coil 206 226 -
5 g5492.t1 Gene3D G3DSA:3.90.226.10 - 28 261 1.1E-51
2 g5492.t1 PANTHER PTHR11941:SF45 ENOYL-COA DELTA ISOMERASE 1, MITOCHONDRIAL 23 284 3.4E-78
3 g5492.t1 PANTHER PTHR11941 ENOYL-COA HYDRATASE-RELATED 23 284 3.4E-78
1 g5492.t1 Pfam PF00378 Enoyl-CoA hydratase/isomerase 42 282 1.7E-36
4 g5492.t1 SUPERFAMILY SSF52096 ClpP/crotonase 31 278 1.09E-46

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

Data is missing for g5492/g5492.t1; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5492.t1.fa.iupred3.txt does not exist

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0003824 catalytic activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values