General information for g553.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as DnaJ homolog subfamily A member 1.
Parent gene
- The parent gene g553 is located on chr_3: 4028825..4030935 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g553 | g553.t2 | isoform | g553.t2 | 4028247 | 4029723 |
| chr_3 | g553 | g553.t2 | exon | g553.t2.exon1 | 4028247 | 4029723 |
| chr_3 | g553 | g553.t2 | TTS | g553.t2 | 4028265 | 4028265 |
| chr_3 | g553 | g553.t2 | cds | g553.t2.CDS1 | 4028825 | 4029691 |
| chr_3 | g553 | g553.t2 | TSS | g553.t2 | NA | NA |
Sequences
>g553.t2 Gene=g553 Length=1477
GCGAACGTAAAGGAAAAGATTTGGTTCACCAAATGCAAGTGTCGTTGGAAGAAATATACA
ATGGAGCTGTACGCAAATTGGCTCTTCAAAAGAATGTCATTTGCGAGAAATGTGAAGGTC
GTGGTGGAAAGAAAGGAGCTGTTGAAACTTGCGGCACTTGTCAAGGCAAAGGTGTTGAAG
TGAAAATTCAGCAAATCATGCCTGGATTTGTTCAAAAACATGAGCAAATTTGCCGGGCAT
GTCAAGGACAAGGAGAAATCATCAATGCAAAAGACCGTTGCAAGACTTGTAATGGCAAAA
AGACAGTGCGTGATAGGAAAATTCTCGAAGTTCATATTGAAAAGGGAATGCAAGATGGTC
AGAGAATAGTCTTCAACGGTGAAGGTGATCAAGAACCCGATTTACAACCAGGCGATATCA
TTATCGTTTTGGATGTGAAAAAGCATCCAGTTTTCCAATTTAGCAATGGAACTGATCTTG
CAATGGTTATGGATCTGCAATTGGTTGAAGCACTCTGCGGTTTTCAGAAAGTCATCAAGA
CACTTGATCAACGTGATTTAGTGATTACTAGCCTTCCAGGTGAAGTAATTAAAGACAATG
AAATCAAGTGTGTGATGGGCGAGGGTATGCCAGAATACAAAAATCCATTCGAGAAAGGTC
GTTTGATTATTCAGTTTAATGTTGTATTTCCAAATGCAATTCCTCCTGAGTATATTCCTA
CTCTTGAACGATGCTTACCTTCACGACCACAAGTTGACATACCCATCACTGCTGAAGAAT
GTATGCTTTCAAATTACGATCCCAATGCAGAGTCACAGCGACGACACAGAGAAGCATATG
AGGAAGATGATGAGCATTATCATCGTGGACCTCGTGTTCAACAATGTGCTACATCGTAAA
TATATAAGAGTCGTAGAATTATTTAACCATCGTTTCTCGCAACAAACCCGGAAATGAAGT
TCTTTAAATTTAAAATTGATTCGTATATATGAAGGAAAAATGCAATTTACCATCCAGCAA
GAAAAAGCATAATAATAACTTATTTCATTCTATTCATATGTATTGCATTTTCAAATCACT
CATATTTTGTTTTCTCACTCTCACTATATTTTATATGCATAATAATAATGTCCTTATTTT
CTCTAAGTATGTGTTGAGATGAACGGAAAAATAAATAGATGAGGATTAAACTGTTAATGT
AAAATTATAATACATAGAAATTACATGCTAGCAATGAAAATAATCACATACCGTAGAATT
TTTATGTTGAACTCATTTTTTTTTGTAACGTTAACAGATATAAATTCACATGCGTCTATG
TTGATTATTAGCTTAACAATTAAAATTTCCTTTGACGTCTATAAGTTAAAATAGATTATT
TGAATTTCTTATTTTTAAGTTCATTCCTAAATTTAAACAGAAACAATACACAATATTCTA
ATAAAGATTATAGAAAATTAATTTAATGGTTTTTCTT
>g553.t2 Gene=g553 Length=288
MQVSLEEIYNGAVRKLALQKNVICEKCEGRGGKKGAVETCGTCQGKGVEVKIQQIMPGFV
QKHEQICRACQGQGEIINAKDRCKTCNGKKTVRDRKILEVHIEKGMQDGQRIVFNGEGDQ
EPDLQPGDIIIVLDVKKHPVFQFSNGTDLAMVMDLQLVEALCGFQKVIKTLDQRDLVITS
LPGEVIKDNEIKCVMGEGMPEYKNPFEKGRLIIQFNVVFPNAIPPEYIPTLERCLPSRPQ
VDIPITAEECMLSNYDPNAESQRRHREAYEEDDEHYHRGPRVQQCATS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g553.t2 | CDD | cd10719 | DnaJ_zf | 24 | 90 | 3.99856E-19 |
| 15 | g553.t2 | CDD | cd10747 | DnaJ_C | 89 | 220 | 2.89422E-40 |
| 12 | g553.t2 | Gene3D | G3DSA:2.60.260.20 | Urease metallochaperone UreE | 1 | 131 | 1.5E-44 |
| 10 | g553.t2 | Gene3D | G3DSA:2.10.230.10 | - | 24 | 90 | 1.5E-44 |
| 11 | g553.t2 | Gene3D | G3DSA:2.60.260.20 | Urease metallochaperone UreE | 143 | 221 | 1.2E-21 |
| 8 | g553.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 256 | 288 | - |
| 9 | g553.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 259 | 280 | - |
| 3 | g553.t2 | PANTHER | PTHR43888 | DNAJ-LIKE-2, ISOFORM A-RELATED | 2 | 283 | 1.8E-114 |
| 4 | g553.t2 | PANTHER | PTHR43888:SF9 | DNAJ HOMOLOG SUBFAMILY A MEMBER 4 | 2 | 283 | 1.8E-114 |
| 1 | g553.t2 | Pfam | PF01556 | DnaJ C terminal domain | 1 | 220 | 2.4E-39 |
| 2 | g553.t2 | Pfam | PF00684 | DnaJ central domain | 24 | 90 | 1.6E-15 |
| 13 | g553.t2 | ProSiteProfiles | PS51188 | Zinc finger CR-type profile. | 11 | 95 | 23.622 |
| 7 | g553.t2 | SUPERFAMILY | SSF49493 | HSP40/DnaJ peptide-binding domain | 1 | 139 | 1.57E-16 |
| 5 | g553.t2 | SUPERFAMILY | SSF57938 | DnaJ/Hsp40 cysteine-rich domain | 21 | 93 | 8.63E-18 |
| 6 | g553.t2 | SUPERFAMILY | SSF49493 | HSP40/DnaJ peptide-binding domain | 145 | 225 | 1.31E-15 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g553/g553.t2; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g553.t2.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006457 | protein folding | BP |
| GO:0051082 | unfolded protein binding | MF |
| GO:0031072 | heat shock protein binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
