Gene loci information

Transcript annotation

  • This transcript has been annotated as Connector enhancer of kinase suppressor of ras 2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g5575 g5575.t1 TSS g5575.t1 10349808 10349808
chr_2 g5575 g5575.t1 isoform g5575.t1 10350059 10354617
chr_2 g5575 g5575.t1 exon g5575.t1.exon1 10350059 10350116
chr_2 g5575 g5575.t1 cds g5575.t1.CDS1 10350059 10350116
chr_2 g5575 g5575.t1 exon g5575.t1.exon2 10350178 10350341
chr_2 g5575 g5575.t1 cds g5575.t1.CDS2 10350178 10350341
chr_2 g5575 g5575.t1 exon g5575.t1.exon3 10350409 10350469
chr_2 g5575 g5575.t1 cds g5575.t1.CDS3 10350409 10350469
chr_2 g5575 g5575.t1 exon g5575.t1.exon4 10350528 10351510
chr_2 g5575 g5575.t1 cds g5575.t1.CDS4 10350528 10351510
chr_2 g5575 g5575.t1 exon g5575.t1.exon5 10351569 10352082
chr_2 g5575 g5575.t1 cds g5575.t1.CDS5 10351569 10352082
chr_2 g5575 g5575.t1 exon g5575.t1.exon6 10352138 10352449
chr_2 g5575 g5575.t1 cds g5575.t1.CDS6 10352138 10352449
chr_2 g5575 g5575.t1 exon g5575.t1.exon7 10352506 10352730
chr_2 g5575 g5575.t1 cds g5575.t1.CDS7 10352506 10352730
chr_2 g5575 g5575.t1 exon g5575.t1.exon8 10352793 10354426
chr_2 g5575 g5575.t1 cds g5575.t1.CDS8 10352793 10354426
chr_2 g5575 g5575.t1 exon g5575.t1.exon9 10354489 10354617
chr_2 g5575 g5575.t1 cds g5575.t1.CDS9 10354489 10354617
chr_2 g5575 g5575.t1 TTS g5575.t1 10355284 10355284

Sequences

>g5575.t1 Gene=g5575 Length=4080
ATGGCATATATTAATGTAAATGATTGGAAATGTGAGCAAGTTATTGAATGGTTAAAAGGA
ATTGAATGCGTAAATAATTTTTACATAAAAGCATTTGCGAATAATCAAATAGGAGGATTT
CAATTGCTCTATATCACACATACAGAACTAAATGAATTGGGTATAAAGAAGATTGGGCAT
CAAGAATTGATATTAGAGGCAGTTGAGCATTTGAAAAATTTTCATTTTAATCTTGAAAAG
GAAAATTTGCAATATCTAGCTCTTCAAGTAGCAACAACGTCCACATCATTATTTAAACAA
CTTTCATATAATCCAGACACAAAACTTGAAAGTGTTGTTTTAAAAGATGTTGCCTACATC
ATATCCAAAGTCAAAACTCTTATTAGTTGGCTCGATAATTCTCCTTTTAAAGGACATTCA
CAATATAGTGAAATGCGTAGAAATTGGCTAAAATTAGGTTTAGAACTCGCGACAATTGCT
CAAAGAGATAGATTTGTCCAAAATCCAATCAAACAAATTACTAGCATAACACAAACTTTA
TCAAAACTTTCTGACTATATTATACATGACATTACTGATCCAATGGTTTTGCAACCTGCT
TTTCTGGATTTGGTGACATTGAAGAAAAAAGAATCAGAGTTAGGCTTCTATATAATGCCT
AATTATCAATCTATTCATAGAGTAACAGATATTAAATATAATTCTCCTGCACATAATTCA
GGAAAAGTAGAAGACGGAGACGAAATTGTGCAAATAAATTATCAAACTGTTATCGGATGG
CAGTATAAAAAAGTGTTGATGCAATTACAAGAATCGACGACTGATGTTTTACTTACATTG
AAAAAGCGGCCAAAACATAGTAATCGAATATACGGTCATCTAGGATTGATTAAATTGCCC
AGTAAAAAAAGATCGATTCCTTATCGTTGGGAACATTTTCCGCCTAGTCCAAGAATAGAA
GAAATGACAGCACCAGAACTCACATTGCCACCAGCAAAGAAAAAAGAAATTATTTTAGAT
CTTACTGATGAATCAAGTGGTGAAAGTGATGTTTTGACTCCAACTGAAATCAAGTCATCT
GATAAAGAACTGCGGTTGTATTTGCCAAAACCGAGAGCTGCAATTCAATTTACGAGGCGT
CATACAATTTGTGGAAATGATTTAAGAAATTTTAAGAATATTGGTAATTTGGTATTGTGG
CATGAGAGGAAGTCAAATAAAACAGATATCGAGTCAAATACAAGTTTACGTGATAAATCT
GTTTCGATTTCATTTGGGCTTAATACTAGACCTTCAACTTGTTTAGGAATTTCTGAAAAT
TCAGGAAAAATTGATCGACTTAAAAGTTCATTACCAGACATTATTAAAAATAGAAAAAAC
AATACTGAAGTGATTCATGAACAAGAGAATGAAAATGACGACAGCGCTTTAAAAACAGGT
GTCAGCAAGGTAGTGAGATTTGACTCTTTAAGTCAAGATTATAATAAAGATTCACAATAT
ACATGCAACGTTGAAGATACCATAATTGAATCATTTGCTCCCATACCTTATGTTGATGAC
GATCCTATTGTTGAAATAAAAAAATGTGATGAGACAAATGTTGAGATAAAAGAGGCAAAA
TCAGTTCAACAACCACCAGCATTAAATCCGAGGACAATTTATGATCATAATTTTAATACA
ATTGTAGAAGCTGTAAATGAAGTCGTAATCGCAAGAGAAACTAAACGAGGAAGATTAGAC
AAAAGTTATAGCACACCAACATATGACCCAACAGAACCAACTAACGAATTGGAATCAATT
GAAACATTATCCATTAATGTTCCACCAAGGCAACAAGCTGTAAAAATTCCTCCTGTGCCA
CCGAGAAAACAAAATATGCAAGCACCCCATCCTCCTCCATCACCTTTCTCTATCTCTATT
GAGGCTAATGATCATGATAATAATAAGTCTAATATAGAGAAACAAAAGAGAGATCAAACA
ACACCAAAAAATAATAACCTAATATTAGAGGCAGAAATTCATATTAAGAAAAGTAGCCCT
ACTGAAATATCAGAATTGCACACACTTACAAAATCTACTAAAAATCAAACGACTTTAACA
AGCAAGAAGAAAAAGTCATTAATGGCAAAGAGAAGAAAAGTAACTTTAAAATCTCTGATG
TCAACAGCTGCTGGTATACAAGGACATTTATACAGGCGTGCAAAAGATAAGAGTGAAGTT
GCATATTGGTCGAAGCTCTATTTTGTTTTAGTCGATACAACTCTATATGGATTTAAAAAT
AAAGAAGCTCAAAAGGCTGACTGTGTAGTTTTTCTAGTTGGATTTACTGTTGGATTAGCA
AGTGAAGTTCATTCAAAAGAGTTTGCATTTAAAGTATATCATCCTAAAAAGACTTTGTAT
CTAGCTGCCGAAACATCTGAAGCAATGACACAATGGATTGATTATATTCGACAAGCGACA
CAAAAAGGAACGACAAATCTTGACTGTGATACAAAGGAACTTTACTCTGAAACAGAGTGT
TCAGACGACGATTTTGACATTTCTTCCAATAAGAATCAATTGACAATCTCTTCACCTGGC
TCTCAATCTCAAACGAGCAATGACAAATCAATTGAGCAAACACCACCATCATCTAAACAT
CATCATTATCATTTGAATTTTGGATCATTAAAAAAGACATTTACGCGTGGAAGTGAAGGA
TCATCTCCATCAGATAATAAATTTTTAGGATTCTTCTCATCAAGTAAAAATGAGAAAAAA
TCAACTGATATTGTCCAAACAGCTCAATTTAAAACCTATAAGAAAGTGAAAGAAATGAAT
GGTGGACTACAACTCGGAGCTACATCAATGATAAATTCAAATGTATCAGATATGTATCTT
TCATCTGGTATTGAAAATTTTACACATCTCTCAACCGACATAAAACATTCTCCGCCTCCG
CAACAAGAAGATACAACTTCAATTAAAACTTCGGAAAGTTCACCGAGTTCAAGTAGTAAT
GATGATAGAAGTAAAAATTCTTCAAAGGGAAATCTTAGAAAAACCCACAATTTTTTGCAT
GCATCAAATCCAAATTTATTAGATTTTAATTTCCATCATACTATCGATTTTCCTATCAAT
AGCGTATCATCAAATACAAATTGGGATTTGAATGGAATGACTTTATTAGATTTTATGAAT
CAACAACGTGAGGAAGAAGAAAAAGATATGTACGATAAAAGAGTTGATCAAGGATTTGAG
AGATCTGAAGATAGATCAGCATTAAAAGTATCAAGTGAAAAACCAGCAATTCCGCAAAAA
CCCAATTCGCACATTGCTAAAATACAAAAACGTACTTTACCACTGCCACCTGATGCAGCA
CAAAGTTTCAAACCTAATGATCAAGCGATATTGTATACGAGAAGTAAGGAAGGACAAAAA
TTGCGAGACTTTGGTTATGAGCTTATTTCAAATGATGATGATCAAAATTCAAATGAGAAA
AATAATGATATAAAATTGTGGGCAAGTAAATCGAAACGCAAAGTTTCACCATTAACTGAA
AGTTTGAAAAAGAAAACAGGATTTAATTGGATGATGATGTCGCATGATAAAGAAGAGTGC
CATACTAATGTTAACTTGGGAAATTCAGGTAGTTTCCGCAAGCTCATGAAATCAAATGAT
GAAAAAACGGCAAAATTGCAAGGTGCTTCAAGCTCAAATATGAGCAGTAAAAATGATAAT
TTTTCATCAATAGTACGCAAAAATTCAGTTCCCAGTAATCCAACTAGTTTGCTCAATAAA
TTATCATTCTCATCATCAAATACAAAAGAAAAGAAATTGCTTGGCTCGCCTAAACTTCAT
CGTGCCATTTTTGGAAGAAATGCTCACTCAAGCACTAGCAGTAATATTAGTACTCAAGCT
TTAAAAGACAATTCAGATCATGAGATATTTACACCTATTACATTCTCAAAACAACCGTCA
AATAATCTGCAACCGTTTCATTTAACACAACATCAAAATTATCCCGATTTAACGCTTCCA
CCCGTTTTTCAACAAGAAACCTACACATTAAATGCATTAAAGAATTTAAAAAATGAATAA

>g5575.t1 Gene=g5575 Length=1359
MAYINVNDWKCEQVIEWLKGIECVNNFYIKAFANNQIGGFQLLYITHTELNELGIKKIGH
QELILEAVEHLKNFHFNLEKENLQYLALQVATTSTSLFKQLSYNPDTKLESVVLKDVAYI
ISKVKTLISWLDNSPFKGHSQYSEMRRNWLKLGLELATIAQRDRFVQNPIKQITSITQTL
SKLSDYIIHDITDPMVLQPAFLDLVTLKKKESELGFYIMPNYQSIHRVTDIKYNSPAHNS
GKVEDGDEIVQINYQTVIGWQYKKVLMQLQESTTDVLLTLKKRPKHSNRIYGHLGLIKLP
SKKRSIPYRWEHFPPSPRIEEMTAPELTLPPAKKKEIILDLTDESSGESDVLTPTEIKSS
DKELRLYLPKPRAAIQFTRRHTICGNDLRNFKNIGNLVLWHERKSNKTDIESNTSLRDKS
VSISFGLNTRPSTCLGISENSGKIDRLKSSLPDIIKNRKNNTEVIHEQENENDDSALKTG
VSKVVRFDSLSQDYNKDSQYTCNVEDTIIESFAPIPYVDDDPIVEIKKCDETNVEIKEAK
SVQQPPALNPRTIYDHNFNTIVEAVNEVVIARETKRGRLDKSYSTPTYDPTEPTNELESI
ETLSINVPPRQQAVKIPPVPPRKQNMQAPHPPPSPFSISIEANDHDNNKSNIEKQKRDQT
TPKNNNLILEAEIHIKKSSPTEISELHTLTKSTKNQTTLTSKKKKSLMAKRRKVTLKSLM
STAAGIQGHLYRRAKDKSEVAYWSKLYFVLVDTTLYGFKNKEAQKADCVVFLVGFTVGLA
SEVHSKEFAFKVYHPKKTLYLAAETSEAMTQWIDYIRQATQKGTTNLDCDTKELYSETEC
SDDDFDISSNKNQLTISSPGSQSQTSNDKSIEQTPPSSKHHHYHLNFGSLKKTFTRGSEG
SSPSDNKFLGFFSSSKNEKKSTDIVQTAQFKTYKKVKEMNGGLQLGATSMINSNVSDMYL
SSGIENFTHLSTDIKHSPPPQQEDTTSIKTSESSPSSSSNDDRSKNSSKGNLRKTHNFLH
ASNPNLLDFNFHHTIDFPINSVSSNTNWDLNGMTLLDFMNQQREEEEKDMYDKRVDQGFE
RSEDRSALKVSSEKPAIPQKPNSHIAKIQKRTLPLPPDAAQSFKPNDQAILYTRSKEGQK
LRDFGYELISNDDDQNSNEKNNDIKLWASKSKRKVSPLTESLKKKTGFNWMMMSHDKEEC
HTNVNLGNSGSFRKLMKSNDEKTAKLQGASSSNMSSKNDNFSSIVRKNSVPSNPTSLLNK
LSFSSSNTKEKKLLGSPKLHRAIFGRNAHSSTSSNISTQALKDNSDHEIFTPITFSKQPS
NNLQPFHLTQHQNYPDLTLPPVFQQETYTLNALKNLKNE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
13 g5575.t1 CDD cd00992 PDZ_signaling 204 281 7.08906E-10
12 g5575.t1 Gene3D G3DSA:1.10.150.50 Transcription Factor 2 85 3.7E-21
11 g5575.t1 Gene3D G3DSA:2.30.42.10 - 201 292 5.2E-13
10 g5575.t1 Gene3D G3DSA:2.30.29.30 - 720 825 4.1E-20
17 g5575.t1 MobiDBLite mobidb-lite consensus disorder prediction 855 881 -
20 g5575.t1 MobiDBLite mobidb-lite consensus disorder prediction 855 873 -
19 g5575.t1 MobiDBLite mobidb-lite consensus disorder prediction 972 1011 -
18 g5575.t1 MobiDBLite mobidb-lite consensus disorder prediction 1079 1098 -
5 g5575.t1 PANTHER PTHR12844 CONNECTOR ENCHANCER OF KINASE SUPPRESSOR OF RAS 1 1160 2.5E-209
6 g5575.t1 PANTHER PTHR12844:SF42 CONNECTOR ENHANCER OF KSR PROTEIN CNK 1 1160 2.5E-209
4 g5575.t1 Pfam PF00536 SAM domain (Sterile alpha motif) 8 72 5.5E-15
2 g5575.t1 Pfam PF10534 Connector enhancer of kinase suppressor of ras 82 163 4.0E-13
3 g5575.t1 Pfam PF00595 PDZ domain 205 280 6.1E-6
1 g5575.t1 Pfam PF00169 PH domain 726 820 6.4E-11
21 g5575.t1 ProSiteProfiles PS50105 SAM domain profile. 9 74 15.582
23 g5575.t1 ProSiteProfiles PS51290 CRIC domain profile. 82 167 14.207
22 g5575.t1 ProSiteProfiles PS50106 PDZ domain profile. 204 284 13.933
24 g5575.t1 ProSiteProfiles PS50003 PH domain profile. 723 821 12.643
16 g5575.t1 SMART SM00454 SAM_4 6 74 1.0E-16
14 g5575.t1 SMART SM00228 pdz_new 212 284 4.8E-6
15 g5575.t1 SMART SM00233 PH_update 724 823 1.8E-13
8 g5575.t1 SUPERFAMILY SSF47769 SAM/Pointed domain 5 79 2.84E-16
9 g5575.t1 SUPERFAMILY SSF50156 PDZ domain-like 176 287 6.05E-16
7 g5575.t1 SUPERFAMILY SSF50729 PH domain-like 700 821 8.49E-24

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

Data is missing for g5575/g5575.t1; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5575.t1.fa.iupred3.txt does not exist

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005515 protein binding MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values