General information for g573.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable cytochrome P450 4d14.
Parent gene
- The parent gene g573 is located on chr_3: 4185745..4187603 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g573 | g573.t1 | isoform | g573.t1 | 4185745 | 4187603 |
| chr_3 | g573 | g573.t1 | exon | g573.t1.exon1 | 4185745 | 4186285 |
| chr_3 | g573 | g573.t1 | cds | g573.t1.CDS1 | 4185745 | 4186285 |
| chr_3 | g573 | g573.t1 | exon | g573.t1.exon2 | 4186352 | 4186413 |
| chr_3 | g573 | g573.t1 | cds | g573.t1.CDS2 | 4186352 | 4186413 |
| chr_3 | g573 | g573.t1 | exon | g573.t1.exon3 | 4186538 | 4186789 |
| chr_3 | g573 | g573.t1 | cds | g573.t1.CDS3 | 4186538 | 4186789 |
| chr_3 | g573 | g573.t1 | exon | g573.t1.exon4 | 4186849 | 4187043 |
| chr_3 | g573 | g573.t1 | cds | g573.t1.CDS4 | 4186849 | 4187043 |
| chr_3 | g573 | g573.t1 | exon | g573.t1.exon5 | 4187170 | 4187375 |
| chr_3 | g573 | g573.t1 | cds | g573.t1.CDS5 | 4187170 | 4187375 |
| chr_3 | g573 | g573.t1 | exon | g573.t1.exon6 | 4187432 | 4187603 |
| chr_3 | g573 | g573.t1 | cds | g573.t1.CDS6 | 4187432 | 4187603 |
| chr_3 | g573 | g573.t1 | TTS | g573.t1 | 4187682 | 4187682 |
| chr_3 | g573 | g573.t1 | TSS | g573.t1 | NA | NA |
Sequences
>g573.t1 Gene=g573 Length=1428
ATGAATGTATTGGCATTATTTTTAATTTTCATCGTTGGTGTTTATTTTTGGTATCAAAAA
AAGTATAAACGATATAATGAACTTATGAACAAAATTCCTTGTGCTAAAACATATCCAATT
TTCAATCACTTAATGTACTTTATCAACAAGAAACCAGCTGAGATTTTTACAGTTCTTAAA
GAAATGGCAAAAGATCATGGACCAGTTTGGAAAGTTTTAGTTCCATTCGGTGATCAAATT
TTCGTTCATGACCCAAAAATCGTTGAAGAAATTTTATCAAGTCAAAAAATTATTGACAAG
TCTATGGAATATGATTTTATAATCGATTGGCTTGGTACAGGACTTTTGATATCAACTGGC
AGTAAATGGCATAAAAGACGAAAGATCATAACACCAGCATTTCATTTTAAAATTCTTGAT
CAGTTTGTTGACATCATGAACAAAAACGGAGATGTTTTTGTCGAAAAATTGAAGCATTTT
GAAGGTCGCGAGATTAACATATATCCATTGATATCACTTTATGCGCTTGATGTTGTTTGT
GAATCTGCTATGGGATATCAACTTAATGCCCAAACTGATGAAGACTCTGAATATGTCAAA
GCGCAATTTTCATCAATGTATAAACGTGAAAAAGAATTGATAAAATTTTTACATAACTTT
ACAGATTCAGTTATTTTTTCACGACGGGAATATTGGAAAAGTCAAGGCATTAATGCTAGT
AATGCTGAAAATGTTGATGAAATTGGAATGAAAAAGAAAACTGCATTTTTGGATTTACTT
TTACAAGTTCAAGTTAACGGTCAGCCACTTGATAATGAAGGAATTCGTGAAGAAGTTGAT
ACTTTTATGTTCGAAGCTCATGACACGACTAGTTCTGCAATTGCTTTTACGCTTTTGAAT
CTAGCAAAATTCAAAGACATTCAGAAGCAAGTTCTTGAAGAATGTGAAGAAATTCTTGGT
GATTTGAGTCAACAACCGACAATGCTAGATCTGAATAAAATGAATTATTTGGAGAAAGTT
ATTAAGGAAACTTTAAGATTGTATCCATCAGTTCCATTATTTTCTAGATCGCTCAAATCC
GAGTTTATCTCAAATGGCTACTCCTTTCCTGAGAATTCGACTGTTGTTTTATCACCTTAT
TTTATGGGTCGAGATGAAAAACTTTTCCCTGATCCTCTGAAATTTGATCCATCAAGATTT
GATGATGAGAAAAATATAAGATTGTTTTCGTTTGTGCCTTTCTCAGCTGGTCCAAGGAAT
TGCATTGGCCAAAAATATGCAATGCTTGAAATGAAAAGCATCATTTCAAAACTTGTAAGA
AATTTCGAAATTGCAATCAGAAAGGAAAATAAAGAGCCACAAATTTATGCACAATTAACT
TTGCAATCAACAAATGGAATAAATTTGTCATTGAAACCCAGAAAATAA
>g573.t1 Gene=g573 Length=475
MNVLALFLIFIVGVYFWYQKKYKRYNELMNKIPCAKTYPIFNHLMYFINKKPAEIFTVLK
EMAKDHGPVWKVLVPFGDQIFVHDPKIVEEILSSQKIIDKSMEYDFIIDWLGTGLLISTG
SKWHKRRKIITPAFHFKILDQFVDIMNKNGDVFVEKLKHFEGREINIYPLISLYALDVVC
ESAMGYQLNAQTDEDSEYVKAQFSSMYKREKELIKFLHNFTDSVIFSRREYWKSQGINAS
NAENVDEIGMKKKTAFLDLLLQVQVNGQPLDNEGIREEVDTFMFEAHDTTSSAIAFTLLN
LAKFKDIQKQVLEECEEILGDLSQQPTMLDLNKMNYLEKVIKETLRLYPSVPLFSRSLKS
EFISNGYSFPENSTVVLSPYFMGRDEKLFPDPLKFDPSRFDDEKNIRLFSFVPFSAGPRN
CIGQKYAMLEMKSIISKLVRNFEIAIRKENKEPQIYAQLTLQSTNGINLSLKPRK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 18 | g573.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 28 | 475 | 1.7E-114 |
| 4 | g573.t1 | PANTHER | PTHR24291:SF143 | CYTOCHROME P450 4D1-RELATED | 6 | 204 | 5.0E-145 |
| 6 | g573.t1 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 6 | 204 | 5.0E-145 |
| 3 | g573.t1 | PANTHER | PTHR24291:SF143 | CYTOCHROME P450 4D1-RELATED | 203 | 474 | 5.0E-145 |
| 5 | g573.t1 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 203 | 474 | 5.0E-145 |
| 7 | g573.t1 | PRINTS | PR00463 | E-class P450 group I signature | 63 | 82 | 5.6E-21 |
| 16 | g573.t1 | PRINTS | PR00385 | P450 superfamily signature | 285 | 302 | 1.7E-12 |
| 9 | g573.t1 | PRINTS | PR00463 | E-class P450 group I signature | 294 | 320 | 5.6E-21 |
| 12 | g573.t1 | PRINTS | PR00463 | E-class P450 group I signature | 338 | 356 | 5.6E-21 |
| 13 | g573.t1 | PRINTS | PR00385 | P450 superfamily signature | 339 | 350 | 1.7E-12 |
| 11 | g573.t1 | PRINTS | PR00463 | E-class P450 group I signature | 378 | 402 | 5.6E-21 |
| 8 | g573.t1 | PRINTS | PR00463 | E-class P450 group I signature | 411 | 421 | 5.6E-21 |
| 14 | g573.t1 | PRINTS | PR00385 | P450 superfamily signature | 412 | 421 | 1.7E-12 |
| 10 | g573.t1 | PRINTS | PR00463 | E-class P450 group I signature | 421 | 444 | 5.6E-21 |
| 15 | g573.t1 | PRINTS | PR00385 | P450 superfamily signature | 421 | 432 | 1.7E-12 |
| 2 | g573.t1 | Pfam | PF00067 | Cytochrome P450 | 36 | 201 | 5.6E-24 |
| 1 | g573.t1 | Pfam | PF00067 | Cytochrome P450 | 203 | 463 | 1.3E-73 |
| 20 | g573.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 21 | g573.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 22 | g573.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 11 | - |
| 23 | g573.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 16 | - |
| 19 | g573.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 475 | - |
| 25 | g573.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 414 | 423 | - |
| 17 | g573.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 23 | 474 | 1.83E-107 |
| 24 | g573.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 2 | 19 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g573/g573.t1; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g573.t1.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K05917 (sterol 14alpha-demethylase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
