General information for g58.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 6A1.
Parent gene
- The parent gene g58 is located on chr_3: 435829..436764 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g58 | g58.t1 | TTS | g58.t1 | 435517 | 435517 |
| chr_3 | g58 | g58.t1 | isoform | g58.t1 | 435829 | 436764 |
| chr_3 | g58 | g58.t1 | exon | g58.t1.exon1 | 435829 | 436764 |
| chr_3 | g58 | g58.t1 | cds | g58.t1.CDS1 | 435829 | 436764 |
| chr_3 | g58 | g58.t1 | TSS | g58.t1 | 436814 | 436814 |
Sequences
>g58.t1 Gene=g58 Length=936
ATGAATTTAGTAAATTTTGCGTTGTACATTATTTTGCCAATAGCGGCAGCAATTTATTTG
TTTTTTAAGAAAAAGTTTTCATATTTTAAGGATCGTGGTATTCCTTATGTTGAGCCTACT
ATTCCACTTGGTAATATGAGTGGATTAGGCTCAAAATATCACTTGGCTGATATTTTACTT
GAGTTCTATGAAAAATGTAAAGAAAAAGGACCAATTGCTGGATTTTACAACACAATTTCT
CCAACATATATCGTTACTGACATCGAACTTGTGACACAAATCACAATTAAAGATTTCAAT
AGCTTTGTCAATCGTGGAATGTATGTCAATGAAGAAGATGAACCCTTAACAGGTCATTTA
GTGTCAATTGAAGATGAAAAATGGAGATTCCTACGAAATAAATTAAGTCCGGTTTTCACT
TCTGGCAAATTAAAAGCCATGTTTAATATAATCAAAGATAAAGGTGACAGTCTCGTGAAA
GCCATTGAAGACTCTTCGAAAAATGGAAAATCAATTGATGCAAAAGATCCAGCAAGTCGT
TTTACGACTGATATAATTTCTTCAACTGCCTTTGGTATGGAATCGAACACACTTAAAGGA
GAACATCTAGAATTATTGAAATTCGCAAAAGAAGTTTTTGGTTCTGAAGGCATAAGCGCA
CTTCAATTTTTCTTCATGATGGCCTACCCAAAAATTTCTAAATTTCTTAAATTGAGACAA
TTTAGCAATGAACTCACAAATTTCTTTACAAAAGTTGTTGGTGATAATATTAAACTTCGT
GAAGAGACCAATGATAAAAGACACGACTTTCTCAACATGTTGATTCAGTTGAAAAATAAA
GGTTCAATTGATGGAGAATTTTCATCAGAAACTCGAAAGTTGACAATGGATGAAGTTACA
GCTCAAGCATTTTTGTTTTTCTTCGCAGTTAAGTAG
>g58.t1 Gene=g58 Length=311
MNLVNFALYIILPIAAAIYLFFKKKFSYFKDRGIPYVEPTIPLGNMSGLGSKYHLADILL
EFYEKCKEKGPIAGFYNTISPTYIVTDIELVTQITIKDFNSFVNRGMYVNEEDEPLTGHL
VSIEDEKWRFLRNKLSPVFTSGKLKAMFNIIKDKGDSLVKAIEDSSKNGKSIDAKDPASR
FTTDIISSTAFGMESNTLKGEHLELLKFAKEVFGSEGISALQFFFMMAYPKISKFLKLRQ
FSNELTNFFTKVVGDNIKLREETNDKRHDFLNMLIQLKNKGSIDGEFSSETRKLTMDEVT
AQAFLFFFAVK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g58.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 17 | 310 | 3.1E-58 |
| 2 | g58.t1 | PANTHER | PTHR24292 | CYTOCHROME P450 | 8 | 309 | 1.8E-90 |
| 3 | g58.t1 | PANTHER | PTHR24292:SF95 | CYP6A16, ISOFORM B-RELATED | 8 | 309 | 1.8E-90 |
| 5 | g58.t1 | PRINTS | PR00464 | Group II E-class P450 signature | 125 | 145 | 3.0E-9 |
| 4 | g58.t1 | PRINTS | PR00464 | Group II E-class P450 signature | 180 | 198 | 3.0E-9 |
| 1 | g58.t1 | Pfam | PF00067 | Cytochrome P450 | 50 | 309 | 4.9E-29 |
| 10 | g58.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 5 | - |
| 11 | g58.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 6 | 22 | - |
| 9 | g58.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 23 | 311 | - |
| 7 | g58.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 26 | 309 | 4.32E-43 |
| 6 | g58.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 5 | 22 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g58/g58.t1; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g58.t1.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0004497 | monooxygenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed