General information for g581.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Probable cytochrome P450 313a4.
Parent gene
- The parent gene g581 is located on chr_3: 4309151..4312262 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g581 | g581.t1 | isoform | g581.t1 | 4307542 | 4309151 |
| chr_3 | g581 | g581.t1 | exon | g581.t1.exon1 | 4307542 | 4307833 |
| chr_3 | g581 | g581.t1 | cds | g581.t1.CDS1 | 4307542 | 4307833 |
| chr_3 | g581 | g581.t1 | exon | g581.t1.exon2 | 4307913 | 4308452 |
| chr_3 | g581 | g581.t1 | cds | g581.t1.CDS2 | 4307913 | 4308452 |
| chr_3 | g581 | g581.t1 | exon | g581.t1.exon3 | 4308512 | 4308920 |
| chr_3 | g581 | g581.t1 | cds | g581.t1.CDS3 | 4308512 | 4308920 |
| chr_3 | g581 | g581.t1 | exon | g581.t1.exon4 | 4308980 | 4309151 |
| chr_3 | g581 | g581.t1 | cds | g581.t1.CDS4 | 4308980 | 4309151 |
| chr_3 | g581 | g581.t1 | TSS | g581.t1 | NA | NA |
| chr_3 | g581 | g581.t1 | TTS | g581.t1 | NA | NA |
Sequences
>g581.t1 Gene=g581 Length=1413
ATGAATTTTATAGTAGTGTCAAGTGTGCTTGTTGCAATTTGTTCAGTACTTGTTGCAATT
TTAATGAGGAAAATCAAAAATTGGATTCCACAAGATTTGGATAAATTTTTTCAATCGGTT
GATCGCCTGTCATCAAAAAAGTTATTTAAAGTGAAATTTTTGTACAAGGACATGTATTTT
GTAAATGATCCAAATCTTATTCATAAAGTCCTAACATCTGATGAGTGCTTAGAAAAGCCA
CAAATGATTTATAAATTTTTCGGTTTAAGTGATGGTTTTCTCTGCTGTAAATTCAATCAA
TGGAAAAGAGATCGCAAAAATTTTAGCTGTTCTTTCAATAATGTTATGGTAAACGCTTTT
ACATCGATATTTTGTGAAATTGCTGATCGAATGGTTGAAAAAATTAAAGAAGTTGGCTGT
GGTCAAAAAGACATAATTTACTTCACTGAACGTTGCACAATTTCAATGATTTTGGCTTCA
TCATTTAATATTTTTCCCAATGATGTTGAGAATAATGAAGAGAGAATTAATGAAGTTGCA
ACAGCTGTAAAAATTCAATCGACTTTACTTTGTGAAAGATTCAGAAAATTTCATCTCTTT
TTTGATGCACTTTTTCGTCACACAAATCATTATCGAAAAATTACAAATCTAAAAAATTAT
CTACTTGAAATTCTTTTTAAAGAAGTCGACAGAAAAAATAGAAGAATTGAAGCAAATAAT
GAGAAGGTTCCAAATTTTGTTGGAAACATTTTCTTCAACAAATTGCTCTTTCATAAAAAT
GAAATGACATTAAATCAATTTAAAGATTCAATTACAACAGTTATTGGCGCTGGTTTTGAA
ACTACTGGAACTGCATCTGCACATTGCATTCTATTTCTTGCAATGCATCCAGAAATTCAA
GAAAAAGCTTATCAAGAAATTCTAAGTATTTATCCTTCAAATGATGATGAAATTACACTA
GAATCTCTCACAAAACTTGAATATATTGAGCGCGTAATGAAAGAAAGTCTACGATTAGGT
CCAACAGTGCATTCAATCGCACGTGAAGCGATGAGAGATTTTGAACTAATTCCAAATAAA
ATTGCGAAAAAAGGATCGATTTTCTGCATAAATATTTACGGCTTGCATAGACGAAAAGAT
TTGTGGGGTGATGATGCTGAGTTGTTTAACCCAGATCGATTTCTACCAGAGAATTTTGAG
GATAAACAAAAGTATTTCCTACCTTTTAGCGTCGGAAGAAGAAATTGCATAGGGTATCGT
TATGCTTACACATCTTTCAAAATTATGATGATAAAATTACTGCGTAATTTTAAATTTACA
ACAAATTTAAAATTAAATGAAATCAAATTTAATCGACAAATTGCTTTAAAGCTTACAGGT
GAACATTTAGTAATGATTGAACAACGAAATTGA
>g581.t1 Gene=g581 Length=470
MNFIVVSSVLVAICSVLVAILMRKIKNWIPQDLDKFFQSVDRLSSKKLFKVKFLYKDMYF
VNDPNLIHKVLTSDECLEKPQMIYKFFGLSDGFLCCKFNQWKRDRKNFSCSFNNVMVNAF
TSIFCEIADRMVEKIKEVGCGQKDIIYFTERCTISMILASSFNIFPNDVENNEERINEVA
TAVKIQSTLLCERFRKFHLFFDALFRHTNHYRKITNLKNYLLEILFKEVDRKNRRIEANN
EKVPNFVGNIFFNKLLFHKNEMTLNQFKDSITTVIGAGFETTGTASAHCILFLAMHPEIQ
EKAYQEILSIYPSNDDEITLESLTKLEYIERVMKESLRLGPTVHSIAREAMRDFELIPNK
IAKKGSIFCINIYGLHRRKDLWGDDAELFNPDRFLPENFEDKQKYFLPFSVGRRNCIGYR
YAYTSFKIMMIKLLRNFKFTTNLKLNEIKFNRQIALKLTGEHLVMIEQRN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g581.t1 | Coils | Coil | Coil | 222 | 242 | - |
| 14 | g581.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 18 | 469 | 2.7E-83 |
| 2 | g581.t1 | PANTHER | PTHR24291:SF140 | CYTOCHROME P450 4C3-RELATED | 53 | 457 | 6.3E-61 |
| 3 | g581.t1 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 53 | 457 | 6.3E-61 |
| 7 | g581.t1 | PRINTS | PR00463 | E-class P450 group I signature | 266 | 283 | 2.2E-14 |
| 12 | g581.t1 | PRINTS | PR00385 | P450 superfamily signature | 277 | 294 | 1.8E-8 |
| 8 | g581.t1 | PRINTS | PR00463 | E-class P450 group I signature | 286 | 312 | 2.2E-14 |
| 11 | g581.t1 | PRINTS | PR00385 | P450 superfamily signature | 331 | 342 | 1.8E-8 |
| 6 | g581.t1 | PRINTS | PR00463 | E-class P450 group I signature | 344 | 362 | 2.2E-14 |
| 5 | g581.t1 | PRINTS | PR00463 | E-class P450 group I signature | 406 | 416 | 2.2E-14 |
| 9 | g581.t1 | PRINTS | PR00385 | P450 superfamily signature | 407 | 416 | 1.8E-8 |
| 4 | g581.t1 | PRINTS | PR00463 | E-class P450 group I signature | 416 | 439 | 2.2E-14 |
| 10 | g581.t1 | PRINTS | PR00385 | P450 superfamily signature | 416 | 427 | 1.8E-8 |
| 1 | g581.t1 | Pfam | PF00067 | Cytochrome P450 | 34 | 449 | 1.1E-66 |
| 17 | g581.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 19 | - |
| 18 | g581.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 19 | g581.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 14 | - |
| 20 | g581.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 19 | - |
| 16 | g581.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 20 | 470 | - |
| 22 | g581.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 409 | 418 | - |
| 13 | g581.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 45 | 469 | 5.89E-81 |
| 21 | g581.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 2 | 21 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g581/g581.t1; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g581.t1.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
