Gene loci information

Transcript annotation

  • This transcript has been annotated as Very long-chain-fatty-acid–CoA ligase bubblegum.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g5884 g5884.t10 isoform g5884.t10 12583568 12585866
chr_2 g5884 g5884.t10 exon g5884.t10.exon1 12583568 12584366
chr_2 g5884 g5884.t10 cds g5884.t10.CDS1 12583649 12584366
chr_2 g5884 g5884.t10 exon g5884.t10.exon2 12584434 12585866
chr_2 g5884 g5884.t10 cds g5884.t10.CDS2 12584434 12585239
chr_2 g5884 g5884.t10 TTS g5884.t10 12585868 12585868
chr_2 g5884 g5884.t10 TSS g5884.t10 NA NA

Sequences

>g5884.t10 Gene=g5884 Length=2232
ACAACAAATTCTGTCGATTCAGTGTTTTATGTACTTGATAGTTGTAAAGCAAATATTGTC
GTTGTTGATGATGCTAAACAAATGGAAAAGATTCATGCAATTAAAGACAAATTGCCTCAT
TTAAAGGCCATCATTCAAACAATTTCACCTTATGCACCTTATGTTAAACGTGCTGATGGT
TATTGGCGATGGAGTGAACTCCAAGAAATGAAGACTGATGATGTCGAAGAAGAATATCAG
CGAAGACTCAAGAGTATAGTTGCAAACGAATGCTGTTGTTTAGTTTATACATCAGGCACA
ACAGGCACACCAAAAGGTGCAATGTTATCGCACGATAATTTTACATGGGATGCTTACAGT
TTCACAGTTTATGTGGATAATTTACAAATGGGAAAGGAAGTTATAGTTAGTTATCTTCCA
CTTTCACATGTTGCTGCACAGATTATTGATATTTTCATTTCAATGACAATTGCTGCTACT
GTATATTTTGCTGATAAAGATACATTAAAAGGATCACTCGTCAAAACACTCATTGATGCA
CGACCAACAAATTTTTTGGGTGTTCCACGTGTTTATGAAAAAATTCAAGAAAAAATGATG
GCTATTGGATCACAGTCGGGTGCATTAAAAAGAGCAATTGCATCATGGGCAAAAAGTGCA
ACATTAAAACATCATATGGAGAAAATGGCTGGTCGACCTTCAGCATCTTTGCAATACAAA
TTAGCAAATAAGATTGTAATGAGCAAAGTTAAGCAAGCACTTGGTCTTAATCGATGCAAA
AACTTTATCACTGGTGCAGCTCCAATGAGCGTAGACACAAAGAAATATTTTATGAGTTTA
GATATGCCAATTGTAGAAGCATTTGGTATGAGTGAATCTACTGGTGGACATTGTATGGGA
AATGTACATGATCCAACATTTGAGTCAATTGGTAGAAATGCACCCGGTACACAGACAAAA
ATTGTGAATGCAGATGAAAAAGGACATGGAGAGATCTGCTTGCGAGGCCGTCATGTTTTT
ATGGGCTATATTAATGAAATTGAAAAGACAATTGATGCTGTTGATGATGAAGGATGGCTT
CATACAGGCGATATTGGATATGTTGACAATGATGGTTATATTTTCATCACTGGAAGACTA
AAGGAAATAATCATCACAGCTGGTGGAGAAAATATTCCTCCAGTTCACATCGAAAACCTC
GTCAAGAGTGAATGTTCAGCCATTTCAAATGCAATACTTATTGGTGATAAAAGAAAATTC
CTTAGTATGTTGATTACATTGAAGACTGAAGTTGATGCAGAAGGAGCACCAAAAAATGAA
CTCACACAAGAAACAATGAAATGGATTCAATCGATGGACTTGAAATACACATCATTAAAA
GAAATTCTCGCAGCAGGACCAGATCCAAAAGTTATGAATGCTATACAAGAAGCTATCAAT
AGAGCTAACAAAAACTCTATTTCAAATGCACAAAAAGTTCAAAAGTTTGCAATTTTACCG
CACGATTTCAGCATACCAACTGGAGAAATCGGACCAACAATGAAAATTAAGCGCAGTTTC
ATTGCAGAAAAATATAAGGAAGTCATCGATAAATTTTATCAATAAGTAATCTCAAAATTT
TTTACGTGTGTCCTTAATTGTGAAACATTCTATTATACTGCTTAAGAACAATGCCAAAAT
TACATCACTTACACACTTTAACTGCCTCACATGATTTTTTGTGAGTCCTTTTATGTTAAT
TTTTTTTCTCATTTTTCTTGGGTTTGATTTTAACTTTTTTTGCTTATGCTCATACCTACC
TATTTATAAATTATCATGAAAGGATCGATTTTTATTAAACTTATTCAATAAGTGTCATTG
TTTTTAAATCATAAATTAATTTATATTGTATATATTTATAGTTATAATGATATGAAGGTA
ATTTGAGCCATTAATTATTATATAAAGAAAAATTTAGAAAATTTTGCTATGCGCTCTGGG
AACTGATATGGGTTAATATTTAATAAATGAGAAAATTTTGAATATACCTATAGCTAGAAG
ATAAGAGTAGTGATGAAAGCCTAAAAGTTGAATCATAAAAGATAGATCTAAATATTAAAG
AAAATTTGTAATAATCTAGTTAATTGAAAAACTTACTTAAGATAATTTTTGTCATTTATC
TTCTTTGTAATGAAGAAAAAATAATCTTATAAATTGATAATAAAATAAAAATCATTGATA
TTGCTAATAAAA

>g5884.t10 Gene=g5884 Length=507
MEKIHAIKDKLPHLKAIIQTISPYAPYVKRADGYWRWSELQEMKTDDVEEEYQRRLKSIV
ANECCCLVYTSGTTGTPKGAMLSHDNFTWDAYSFTVYVDNLQMGKEVIVSYLPLSHVAAQ
IIDIFISMTIAATVYFADKDTLKGSLVKTLIDARPTNFLGVPRVYEKIQEKMMAIGSQSG
ALKRAIASWAKSATLKHHMEKMAGRPSASLQYKLANKIVMSKVKQALGLNRCKNFITGAA
PMSVDTKKYFMSLDMPIVEAFGMSESTGGHCMGNVHDPTFESIGRNAPGTQTKIVNADEK
GHGEICLRGRHVFMGYINEIEKTIDAVDDEGWLHTGDIGYVDNDGYIFITGRLKEIIITA
GGENIPPVHIENLVKSECSAISNAILIGDKRKFLSMLITLKTEVDAEGAPKNELTQETMK
WIQSMDLKYTSLKEILAAGPDPKVMNAIQEAINRANKNSISNAQKVQKFAILPHDFSIPT
GEIGPTMKIKRSFIAEKYKEVIDKFYQ

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g5884.t10 Gene3D G3DSA:3.40.50.12780 - 1 206 1.6E-25
7 g5884.t10 Gene3D G3DSA:3.40.50.12780 - 209 352 5.5E-35
2 g5884.t10 PANTHER PTHR43272 LONG-CHAIN-FATTY-ACID–COA LIGASE 2 506 2.1E-199
3 g5884.t10 PANTHER PTHR43272:SF32 LONG-CHAIN-FATTY-ACID–COA LIGASE ACSBG2 2 506 2.1E-199
1 g5884.t10 Pfam PF00501 AMP-binding enzyme 41 359 7.3E-66
5 g5884.t10 ProSitePatterns PS00455 Putative AMP-binding domain signature. 67 78 -
4 g5884.t10 SUPERFAMILY SSF56801 Acetyl-CoA synthetase-like 3 506 8.77E-76

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

Data is missing for g5884/g5884.t10; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5884.t10.fa.iupred3.txt does not exist

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values