Gene loci information

Transcript annotation

  • This transcript has been annotated as Very long-chain-fatty-acid–CoA ligase bubblegum.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g5884 g5884.t2 TSS g5884.t2 12578293 12578293
chr_2 g5884 g5884.t2 isoform g5884.t2 12578386 12584366
chr_2 g5884 g5884.t2 exon g5884.t2.exon1 12578386 12578388
chr_2 g5884 g5884.t2 exon g5884.t2.exon2 12583062 12583476
chr_2 g5884 g5884.t2 cds g5884.t2.CDS1 12583124 12583476
chr_2 g5884 g5884.t2 exon g5884.t2.exon3 12583546 12584366
chr_2 g5884 g5884.t2 cds g5884.t2.CDS2 12583546 12584365
chr_2 g5884 g5884.t2 TTS g5884.t2 NA NA

Sequences

>g5884.t2 Gene=g5884 Length=1239
TAGATATATATTGAAAAATTAGTGACTTTTCTGCGTAATATAATTAAACAAAAAAATTAA
ACAACATGGCAAAACACGTTGCATTACGCGAAACGAAAGAATTTCGCATAACAGATCCAT
CAAAACCCGTGAGAATATTTCAAAATCCACGTGATAAAACAGCATCGGTCGATCCTATTA
CAGTTCCAGCTCTCATGAATCGTACAGTAGAAAATTATGGTAATCATCCAGCTCTCAAAT
ACAAAGATCCTGTCACAGGAAAGTGGCAGACAATTACTTATAAAGAATACAAAGAACGCG
TTGAGAAGATGGCTAAAGTTTTCATAAAACTTGGTCTCGAACGCTATGGCACTGTCGCAG
TTCTCGCATTCAATAGTGTTGAATGGTTTGTCTCTGAATTAGCGGCAATTCATGCCGGTG
GAAATATTGCTGGTGTATACACAACAAATTCTGTCGATTCAGTGTTTTATGTACTTGATA
GTTGTAAAGCAAATATTGTCGTTGTTGATGATGCTAAACAAATGGAAAAGATTCATGCAA
TTAAAGACAAATTGCCTCATTTAAAGGCCATCATTCAAACAATTTCACCTTATGCACCTT
ATGTTAAACGTGCTGATGGTTATTGGCGATGGAGTGAACTCCAAGAAATGAAGACTGATG
ATGTCGAAGAAGAATATCAGCGAAGACTCAAGAGTATAGTTGCAAACGAATGCTGTTGTT
TAGTTTATACATCAGGCACAACAGGCACACCAAAAGGTGCAATGTTATCGCACGATAATT
TTACATGGGATGCTTACAGTTTCACAGTTTATGTGGATAATTTACAAATGGGAAAGGAAG
TTATAGTTAGTTATCTTCCACTTTCACATGTTGCTGCACAGATTATTGATATTTTCATTT
CAATGACAATTGCTGCTACTGTATATTTTGCTGATAAAGATACATTAAAAGGATCACTCG
TCAAAACACTCATTGATGCACGACCAACAAATTTTTTGGGTGTTCCACGTGTTTATGAAA
AAATTCAAGAAAAAATGATGGCTATTGGATCACAGTCGGGTGCATTAAAAAGAGCAATTG
CATCATGGGCAAAAAGTGCAACATTAAAACATCATATGGAGAAAATGGCTGGTCGACCTT
CAGCATCTTTGCAATACAAATTAGCAAATAAGATTGTAATGAGCAAAGTTAAGCAAGCAC
TTGGTCTTAATCGATGCAAAAACTTTATCACTGGTGCAG

>g5884.t2 Gene=g5884 Length=391
MAKHVALRETKEFRITDPSKPVRIFQNPRDKTASVDPITVPALMNRTVENYGNHPALKYK
DPVTGKWQTITYKEYKERVEKMAKVFIKLGLERYGTVAVLAFNSVEWFVSELAAIHAGGN
IAGVYTTNSVDSVFYVLDSCKANIVVVDDAKQMEKIHAIKDKLPHLKAIIQTISPYAPYV
KRADGYWRWSELQEMKTDDVEEEYQRRLKSIVANECCCLVYTSGTTGTPKGAMLSHDNFT
WDAYSFTVYVDNLQMGKEVIVSYLPLSHVAAQIIDIFISMTIAATVYFADKDTLKGSLVK
TLIDARPTNFLGVPRVYEKIQEKMMAIGSQSGALKRAIASWAKSATLKHHMEKMAGRPSA
SLQYKLANKIVMSKVKQALGLNRCKNFITGA

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g5884.t2 Gene3D G3DSA:3.40.50.12780 - 33 362 1.4E-51
2 g5884.t2 PANTHER PTHR43272 LONG-CHAIN-FATTY-ACID–COA LIGASE 9 391 1.8E-127
3 g5884.t2 PANTHER PTHR43272:SF32 LONG-CHAIN-FATTY-ACID–COA LIGASE ACSBG2 9 391 1.8E-127
1 g5884.t2 Pfam PF00501 AMP-binding enzyme 46 338 8.7E-56
5 g5884.t2 ProSitePatterns PS00455 Putative AMP-binding domain signature. 219 230 -
4 g5884.t2 SUPERFAMILY SSF56801 Acetyl-CoA synthetase-like 35 338 2.75E-55

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

Data is missing for g5884/g5884.t2; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5884.t2.fa.iupred3.txt does not exist

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values