General information for g602.t20
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Prosaposin.
Parent gene
- The parent gene g602 is located on chr_3: 4437608..4441569 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g602 | g602.t20 | TSS | g602.t20 | 4434335 | 4434335 |
| chr_3 | g602 | g602.t20 | isoform | g602.t20 | 4435080 | 4437608 |
| chr_3 | g602 | g602.t20 | exon | g602.t20.exon1 | 4435080 | 4435168 |
| chr_3 | g602 | g602.t20 | cds | g602.t20.CDS1 | 4435157 | 4435168 |
| chr_3 | g602 | g602.t20 | exon | g602.t20.exon2 | 4435272 | 4437497 |
| chr_3 | g602 | g602.t20 | cds | g602.t20.CDS2 | 4435272 | 4437497 |
| chr_3 | g602 | g602.t20 | exon | g602.t20.exon3 | 4437561 | 4437608 |
| chr_3 | g602 | g602.t20 | cds | g602.t20.CDS3 | 4437561 | 4437608 |
| chr_3 | g602 | g602.t20 | TTS | g602.t20 | 4437776 | 4437776 |
Sequences
>g602.t20 Gene=g602 Length=2363
TAAAAGTAGTAAAAACGGAATGCAAACGTTTGGCTGATGATTTCATACCAGAACTTGTTG
AGGCACTTGCTTCTCAAATGAATCCACAGGTCGTTTGTTCTACAGCTGGACTTTGTAATA
ATGCAAAGATTGATGCATTATTGGAAGAATATGAAATGCAGAAGAAAAATAATTTATTAA
GTTGCAACCAATGTAACAACATTGGAGAATTGATCACTCAAAAATTCCATACATCATCTC
GTGATCAAGTTCTAGATGGTTTCTTGAGTGCCTGCGGTCGTCTTTCATCGTTTTCTGATG
CATGTTCTAGTATCATTCTGACTTATTTCAACGAAATCTATGAAGAACTTTCGAGAAATC
TAAATTCTGATAGTATTTGTCATATATCTGGCGTATGCGCAAGCAAATATCATAATCATG
CATCAGCAGAAGTTGAAATTGAAGTAAAATCAAATGTTGGAGTACTTAAGAATAAAGAGC
TCAAAGATGATATTCCTTGTGAATTATGTGAACAGTTGGTCAAACATTTAAGTGATATTC
TTGTTGCAAATACAACTGAAACTGAATTTAAAAGCGTCCTTCAAGGTCTTTGTTCACAAA
CAAAAGGATTCAAGAGCGAATGTTTAGAACTGGTAAATCAATATTATGCAATTATTTACG
AGTCTCTCGTTAATAATCTCGACGCAAATGGAGCTTGCTTTTTAATTGGAGTATGTCGCA
AGGGAGATAATAATCATCAAGTCACTCCACACATGCCATTATTGCCTACTAATCAAAAGA
ATATACCAAAGCGCAATCGATTAGGAGAGAATGAAAAATCCTATACAAATGAGCAAATCA
AGAAAATGATTTTGCCTATTGATCAATTAATGGGAGCTAAATCAAACCTTGATTTGGTTG
ATGGTGGTGAAAAGTGTACTCTTTGTCAATATTTTCTTCATTTTGTTCAAGAAGAATTAA
GTGATGCAAAAAATGAAGACGAAATTAAAGAGATAGTAGGAAAAACTTGTGACAAATTCT
CATCCGTAATTCGTTCAAATTGTCATCAATTTGTTAATTTATATGGAGATGCAATAATTG
CTTTATTAGTTCAAAGCATTGATCCTCGCGATGTATGTCCCAATTTAAAATTATGTCCGA
AATTCATGAATCAAGACGTTGATGTTTTTGCACCTTATCCGATCGTTGAAGTAAATGAAT
CCAGCAAAACGCAATGTCCATTGTGTGTTCTTGTTGTAAAAGAGGCTCAAGATTATATAA
AAAGTGAGAAAACTACTGAAACCGTAAAGAAAGCACTAGAAAGAGCGTGTTCGAGACTCC
CAGCAAAATTACAATTGCAATGTAAAGATTTTGTGGAAACTTATTACGATGAATTACTTC
AAAAGTTGCTCTCTGATTTCACTCCAAAAGACATTTGCATTGATTTGCAATTGTGCACAA
GAATTCAAGATGAAATAAAAGTTGGAATTGAAAAAGTCGAACATATTCCAAACATCAAGA
CAAATGAAATTCCTGATTATACTATCAACGGTCAATATTTTACAAGTTCTGATTCTGGAG
AGTGCTTCTTGTGTACAACCGTTTTAAGTAACGCTGAGAGCAAAATTACTCATGGAATGA
CTAAGAATCAAATTGAAGATATTTTATTACGCGAATGCTCAGTTTATTATATTTATGAAG
GCGTCTGCGATAATTTTGTTAAAACGAATATTGATCAAATATTCTCTTTAGTAGAACAAA
AACTTTCTCCTAAGCAAGTTTGTCAGAATTTATCTCTCTGTGCATCAACAGACGAGTTAA
ATATTGATGAAGCAGTTATTGTAAATGTTGTGGCATTACCTGCATTCCCAACTGAAAAGA
AAAATGAATTTAATCGTGTTAAAATTATAAGAGGAGATGATGATGATGATGATGATGATG
ACGACGACGATGATGATGATGATGATGATGATGATGATGATCCAAAGAAAAAACCTTCAT
TCAAAGATGATCCAATTTGTGTACTTTGCGAATTTGTAATGACAAAATTAGAATCTGAAT
TGAAAAATAAAAAAACACAGGAAGAAATCAAAGAGGCTGTTGAGAATATTTGCTCAAAAA
TGCCGAAATCTGTTTCTAAGCAATGTACAAAATTCGTTGATCAATACAGTGAACTCATCA
TTGCATTGATTGATACTGTACCACCCAAAGAAATTTGTTCACAAATGGGCTTATGTACAG
CTGTAAAAAAACAAATGCACCTCGTGGGTGCAAATGAGTGCACTTATGGTCCAACTCACT
TTTGTTCTGATATGAAAATTGCTGAAAAATGCAAGGCTACCAAATACTGTAAGGAAAAGA
AGTTGGGAAAATTTTCAGTTTGA
>g602.t20 Gene=g602 Length=761
MNPQVVCSTAGLCNNAKIDALLEEYEMQKKNNLLSCNQCNNIGELITQKFHTSSRDQVLD
GFLSACGRLSSFSDACSSIILTYFNEIYEELSRNLNSDSICHISGVCASKYHNHASAEVE
IEVKSNVGVLKNKELKDDIPCELCEQLVKHLSDILVANTTETEFKSVLQGLCSQTKGFKS
ECLELVNQYYAIIYESLVNNLDANGACFLIGVCRKGDNNHQVTPHMPLLPTNQKNIPKRN
RLGENEKSYTNEQIKKMILPIDQLMGAKSNLDLVDGGEKCTLCQYFLHFVQEELSDAKNE
DEIKEIVGKTCDKFSSVIRSNCHQFVNLYGDAIIALLVQSIDPRDVCPNLKLCPKFMNQD
VDVFAPYPIVEVNESSKTQCPLCVLVVKEAQDYIKSEKTTETVKKALERACSRLPAKLQL
QCKDFVETYYDELLQKLLSDFTPKDICIDLQLCTRIQDEIKVGIEKVEHIPNIKTNEIPD
YTINGQYFTSSDSGECFLCTTVLSNAESKITHGMTKNQIEDILLRECSVYYIYEGVCDNF
VKTNIDQIFSLVEQKLSPKQVCQNLSLCASTDELNIDEAVIVNVVALPAFPTEKKNEFNR
VKIIRGDDDDDDDDDDDDDDDDDDDDDDPKKKPSFKDDPICVLCEFVMTKLESELKNKKT
QEEIKEAVENICSKMPKSVSKQCTKFVDQYSELIIALIDTVPPKEICSQMGLCTAVKKQM
HLVGANECTYGPTHFCSDMKIAEKCKATKYCKEKKLGKFSV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 34 | g602.t20 | Coils | Coil | Coil | 650 | 670 | - |
| 30 | g602.t20 | Gene3D | G3DSA:1.10.225.10 | Saposin | 32 | 116 | 8.9E-11 |
| 32 | g602.t20 | Gene3D | G3DSA:1.10.225.10 | Saposin | 135 | 215 | 4.2E-17 |
| 28 | g602.t20 | Gene3D | G3DSA:1.10.225.10 | Saposin | 276 | 353 | 3.3E-21 |
| 29 | g602.t20 | Gene3D | G3DSA:1.10.225.10 | Saposin | 375 | 455 | 1.1E-20 |
| 33 | g602.t20 | Gene3D | G3DSA:1.10.225.10 | Saposin | 491 | 574 | 7.5E-16 |
| 31 | g602.t20 | Gene3D | G3DSA:1.10.225.10 | Saposin | 635 | 715 | 3.0E-23 |
| 42 | g602.t20 | MobiDBLite | mobidb-lite | consensus disorder prediction | 607 | 632 | - |
| 43 | g602.t20 | MobiDBLite | mobidb-lite | consensus disorder prediction | 608 | 627 | - |
| 12 | g602.t20 | PANTHER | PTHR11480 | SAPOSIN-RELATED | 1 | 223 | 1.1E-121 |
| 15 | g602.t20 | PANTHER | PTHR11480:SF84 | BCDNA.GH08312 | 1 | 223 | 1.1E-121 |
| 11 | g602.t20 | PANTHER | PTHR11480 | SAPOSIN-RELATED | 256 | 459 | 1.1E-121 |
| 14 | g602.t20 | PANTHER | PTHR11480:SF84 | BCDNA.GH08312 | 256 | 459 | 1.1E-121 |
| 13 | g602.t20 | PANTHER | PTHR11480 | SAPOSIN-RELATED | 491 | 716 | 1.1E-121 |
| 16 | g602.t20 | PANTHER | PTHR11480:SF84 | BCDNA.GH08312 | 491 | 716 | 1.1E-121 |
| 20 | g602.t20 | PRINTS | PR01797 | Saposin signature | 125 | 145 | 1.0E-10 |
| 22 | g602.t20 | PRINTS | PR01797 | Saposin signature | 155 | 172 | 1.0E-10 |
| 21 | g602.t20 | PRINTS | PR01797 | Saposin signature | 380 | 400 | 1.0E-10 |
| 19 | g602.t20 | PRINTS | PR01797 | Saposin signature | 429 | 447 | 1.0E-10 |
| 18 | g602.t20 | PRINTS | PR01797 | Saposin signature | 493 | 515 | 1.0E-10 |
| 17 | g602.t20 | PRINTS | PR01797 | Saposin signature | 546 | 569 | 1.0E-10 |
| 4 | g602.t20 | Pfam | PF05184 | Saposin-like type B, region 1 | 140 | 174 | 1.0E-6 |
| 9 | g602.t20 | Pfam | PF03489 | Saposin-like type B, region 2 | 181 | 213 | 2.1E-5 |
| 3 | g602.t20 | Pfam | PF05184 | Saposin-like type B, region 1 | 279 | 314 | 2.5E-7 |
| 6 | g602.t20 | Pfam | PF03489 | Saposin-like type B, region 2 | 322 | 353 | 1.1E-7 |
| 1 | g602.t20 | Pfam | PF05184 | Saposin-like type B, region 1 | 379 | 415 | 1.1E-10 |
| 5 | g602.t20 | Pfam | PF03489 | Saposin-like type B, region 2 | 421 | 453 | 1.1E-9 |
| 7 | g602.t20 | Pfam | PF03489 | Saposin-like type B, region 2 | 536 | 568 | 2.8E-8 |
| 2 | g602.t20 | Pfam | PF05184 | Saposin-like type B, region 1 | 639 | 676 | 1.6E-13 |
| 8 | g602.t20 | Pfam | PF03489 | Saposin-like type B, region 2 | 681 | 713 | 1.2E-9 |
| 10 | g602.t20 | Pfam | PF02199 | Saposin A-type domain | 726 | 754 | 9.5E-9 |
| 50 | g602.t20 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 32 | 111 | 11.733 |
| 47 | g602.t20 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 137 | 217 | 15.929 |
| 49 | g602.t20 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 276 | 357 | 17.318 |
| 46 | g602.t20 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 376 | 457 | 19.58 |
| 45 | g602.t20 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 492 | 572 | 15.258 |
| 48 | g602.t20 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 637 | 717 | 19.907 |
| 44 | g602.t20 | ProSiteProfiles | PS51110 | Saposin A-type domain profile. | 721 | 761 | 10.22 |
| 39 | g602.t20 | SMART | SM00741 | sapb_4 | 34 | 107 | 1.2E-5 |
| 37 | g602.t20 | SMART | SM00741 | sapb_4 | 139 | 213 | 1.0E-15 |
| 41 | g602.t20 | SMART | SM00741 | sapb_4 | 278 | 353 | 4.0E-19 |
| 38 | g602.t20 | SMART | SM00741 | sapb_4 | 378 | 453 | 2.1E-22 |
| 36 | g602.t20 | SMART | SM00741 | sapb_4 | 494 | 568 | 1.2E-15 |
| 40 | g602.t20 | SMART | SM00741 | sapb_4 | 639 | 713 | 1.3E-25 |
| 35 | g602.t20 | SMART | SM00162 | sapA_2 | 724 | 757 | 0.0074 |
| 27 | g602.t20 | SUPERFAMILY | SSF47862 | Saposin | 72 | 173 | 9.29E-15 |
| 26 | g602.t20 | SUPERFAMILY | SSF47862 | Saposin | 178 | 314 | 1.18E-16 |
| 25 | g602.t20 | SUPERFAMILY | SSF47862 | Saposin | 319 | 415 | 3.14E-19 |
| 24 | g602.t20 | SUPERFAMILY | SSF47862 | Saposin | 493 | 570 | 3.4E-14 |
| 23 | g602.t20 | SUPERFAMILY | SSF47862 | Saposin | 637 | 714 | 5.94E-20 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006665 | sphingolipid metabolic process | BP |
| GO:0005764 | lysosome | CC |
| GO:0006629 | lipid metabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K12382 (saposin)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
