General information for g6226.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Apyrase.
Parent gene
- The parent gene g6226 is located on chr_2: 15411401..15413465 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6226 | g6226.t1 | TTS | g6226.t1 | 15411319 | 15411319 |
| chr_2 | g6226 | g6226.t1 | isoform | g6226.t1 | 15411401 | 15413465 |
| chr_2 | g6226 | g6226.t1 | exon | g6226.t1.exon1 | 15411401 | 15412329 |
| chr_2 | g6226 | g6226.t1 | cds | g6226.t1.CDS1 | 15411401 | 15412329 |
| chr_2 | g6226 | g6226.t1 | exon | g6226.t1.exon2 | 15412386 | 15412824 |
| chr_2 | g6226 | g6226.t1 | cds | g6226.t1.CDS2 | 15412386 | 15412824 |
| chr_2 | g6226 | g6226.t1 | exon | g6226.t1.exon3 | 15413122 | 15413269 |
| chr_2 | g6226 | g6226.t1 | cds | g6226.t1.CDS3 | 15413122 | 15413269 |
| chr_2 | g6226 | g6226.t1 | exon | g6226.t1.exon4 | 15413332 | 15413465 |
| chr_2 | g6226 | g6226.t1 | cds | g6226.t1.CDS4 | 15413332 | 15413465 |
| chr_2 | g6226 | g6226.t1 | TSS | g6226.t1 | 15413516 | 15413516 |
Sequences
>g6226.t1 Gene=g6226 Length=1650
ATGCATAAATTTTCAAGTGTTTTTCTAATCGTTTTACTCCTGAGAATTTCACCATCTTTA
CCACAAAAAAACATTGCATTTGATGACAGTTGGTTTCCTTTAAGCATCATCCATATAAAT
GATTTTCATGCAAGATTCGATGAAACAAATAATCAATCAAATGACTGTAAACCTGAAATA
GAGGAATGTATTGGTGGATATGCACGTGTTGTGACGACTGTTAAAGAATTACTGAAAACA
AGAGAAAATCCACTTTATTTAAATGCAGGAGATAATTTTCAGGGAACATTATGGTATAAT
ATCGGTCGTTGGAATGTTACAAATCAATTTCTTAACATGCTTCCTGCTGATGCACAAACA
ATTGGCAATCACGATTTCGATCATGGTGTTGAAGGTGTAATTCCATTTTTAAATCGAACT
TTAACGCCTGTAGTCATTGCAAATGTTGATGACTCAAATGAACCATCATTTCAAGGAATG
TATAAAAAATCAATTATAATTACAAAATATGGAAGAAAAATTGGAATCATTGGTGCAATT
TTAAGAAGCACTAACACAATAGCAAAAACTGGAAATATAAAATTTTTCAATGAAGCAGAA
AAAGTCAGAGAAGAAGCAGCTCGATTAAGAACTGAAGAAGGTGTAAACATTATTATTGTC
ATTTCACATTGCGGTCTTGATCGTGATCGTGAAATTGCACTCAATGGTGGACCTGATATT
GATATCATTGTCGGTGGTCACAGTCATTCATTTTTATGGAATGGAACTGATTATCCAGCA
CTTGATCGTCCTGCTGCTAATTATCCAGTTGTTGTTAATCAAAACGATGGACATAGAGTT
TTAATTGTTCAAGCATCAGCTTATACAAAATATGTTGGTGACATAACTCTCTATTTCGAT
GATGAAGGAATAATTCAATCATGGAATGGAAATCCAATTTTTATGGGATCAAATGTTGCT
CAAGATCCTGAAGTTTTAGCAGCTATCCAACCATGGAAACAAATTATTGATATGGAAGGT
CAAAAGATTGTTGGAAGTATTAAATTCACAGCATCAAGTTCTGGCTGTTACAATGGAGAT
TGTTTAATGGGAAATTTGCAAGCTGATGCAATGGCTTATTCTGCTTTCTTGGAAGATGAT
GAAGAAGGAGCTTGGACTTATGCAACAATTGCAATTACTAATCCCGGTGGCGTAAGAGGA
TCTTTGATATCTGGTGAACTTTCTTATAGTAATTTAGTCACAACAACTCCATTTGAAAAC
ACTTGCGATAGAGCTGAAGTGCAGGGAAAACACATTAGAGAAGCACTTGAATTTAGTGTT
CGAACTTCAAGTCCAAGTGTTTTACAAACATCAGGAATTCAAGTTGTATTCAATAGAACA
AGACCAGCATACAGTCGTGTACAATCATTGAAAGTTCTTTGCAGACTTTGTGATGTACCA
CGATATGAAGATATTGAAGATGAAACTTGGTATCGTGTTATTATCAATAATTTCTTATTT
CAAAATGGCGACAATTTTGCTATGCTTAGAGACAACATGAGAAATCACAAAGTTGGAGAT
GTTGACATTGATGCTTTAAAAACTTACATTGAAAGAAATTCACCAATTACTATGTTGCCA
CCTCGTGGTCGTGTTACATTTGTGACTTAA
>g6226.t1 Gene=g6226 Length=549
MHKFSSVFLIVLLLRISPSLPQKNIAFDDSWFPLSIIHINDFHARFDETNNQSNDCKPEI
EECIGGYARVVTTVKELLKTRENPLYLNAGDNFQGTLWYNIGRWNVTNQFLNMLPADAQT
IGNHDFDHGVEGVIPFLNRTLTPVVIANVDDSNEPSFQGMYKKSIIITKYGRKIGIIGAI
LRSTNTIAKTGNIKFFNEAEKVREEAARLRTEEGVNIIIVISHCGLDRDREIALNGGPDI
DIIVGGHSHSFLWNGTDYPALDRPAANYPVVVNQNDGHRVLIVQASAYTKYVGDITLYFD
DEGIIQSWNGNPIFMGSNVAQDPEVLAAIQPWKQIIDMEGQKIVGSIKFTASSSGCYNGD
CLMGNLQADAMAYSAFLEDDEEGAWTYATIAITNPGGVRGSLISGELSYSNLVTTTPFEN
TCDRAEVQGKHIREALEFSVRTSSPSVLQTSGIQVVFNRTRPAYSRVQSLKVLCRLCDVP
RYEDIEDETWYRVIINNFLFQNGDNFAMLRDNMRNHKVGDVDIDALKTYIERNSPITMLP
PRGRVTFVT
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 22 | g6226.t1 | CDD | cd07409 | MPP_CD73_N | 34 | 315 | 3.27522E-147 |
| 15 | g6226.t1 | Gene3D | G3DSA:3.60.21.10 | - | 32 | 339 | 8.1E-91 |
| 16 | g6226.t1 | Gene3D | G3DSA:3.90.780.10 | - | 345 | 547 | 2.9E-49 |
| 3 | g6226.t1 | PANTHER | PTHR11575 | 5’-NUCLEOTIDASE-RELATED | 10 | 537 | 3.4E-210 |
| 4 | g6226.t1 | PANTHER | PTHR11575:SF32 | APYRASE-LIKE PROTEIN | 10 | 537 | 3.4E-210 |
| 7 | g6226.t1 | PRINTS | PR01607 | Apyrase family signature | 32 | 50 | 3.5E-31 |
| 10 | g6226.t1 | PRINTS | PR01607 | Apyrase family signature | 213 | 230 | 3.5E-31 |
| 5 | g6226.t1 | PRINTS | PR01607 | Apyrase family signature | 233 | 256 | 3.5E-31 |
| 6 | g6226.t1 | PRINTS | PR01607 | Apyrase family signature | 280 | 300 | 3.5E-31 |
| 9 | g6226.t1 | PRINTS | PR01607 | Apyrase family signature | 406 | 429 | 3.5E-31 |
| 8 | g6226.t1 | PRINTS | PR01607 | Apyrase family signature | 484 | 503 | 3.5E-31 |
| 2 | g6226.t1 | Pfam | PF00149 | Calcineurin-like phosphoesterase | 35 | 250 | 2.1E-14 |
| 1 | g6226.t1 | Pfam | PF02872 | 5’-nucleotidase, C-terminal domain | 355 | 510 | 5.9E-32 |
| 18 | g6226.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 21 | - |
| 19 | g6226.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 20 | g6226.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 14 | - |
| 21 | g6226.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 21 | - |
| 17 | g6226.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 22 | 549 | - |
| 23 | g6226.t1 | ProSitePatterns | PS00785 | 5’-nucleotidase signature 1. | 34 | 46 | - |
| 12 | g6226.t1 | SUPERFAMILY | SSF56300 | Metallo-dependent phosphatases | 33 | 336 | 6.2E-82 |
| 11 | g6226.t1 | SUPERFAMILY | SSF55816 | 5’-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain | 345 | 545 | 1.83E-42 |
| 14 | g6226.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
| 13 | g6226.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 26 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0046872 | metal ion binding | MF |
| GO:0016787 | hydrolase activity | MF |
| GO:0000166 | nucleotide binding | MF |
| GO:0016788 | hydrolase activity, acting on ester bonds | MF |
| GO:0009166 | nucleotide catabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K01081 (5’-nucleotidase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed