General information for g6281.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Hematopoietically-expressed homeobox protein hhex.
Parent gene
- The parent gene g6281 is located on chr_2: 15808624..15809517 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6281 | g6281.t2 | isoform | g6281.t2 | 15802774 | 15809517 |
| chr_2 | g6281 | g6281.t2 | exon | g6281.t2.exon1 | 15802774 | 15803255 |
| chr_2 | g6281 | g6281.t2 | cds | g6281.t2.CDS1 | 15802774 | 15803255 |
| chr_2 | g6281 | g6281.t2 | exon | g6281.t2.exon2 | 15809115 | 15809517 |
| chr_2 | g6281 | g6281.t2 | cds | g6281.t2.CDS2 | 15809115 | 15809517 |
| chr_2 | g6281 | g6281.t2 | TSS | g6281.t2 | 15809664 | 15809664 |
| chr_2 | g6281 | g6281.t2 | TTS | g6281.t2 | NA | NA |
Sequences
>g6281.t2 Gene=g6281 Length=885
ATGTATCCATTTTCAAAGAATAAAGCTTCATTTAGAATCGCAGATATTTTACATCAACAC
CAGCAACAACAACAACAGCAGCAGCAGCAAACGGCAGCAGATAATCAATTACTTGCACAA
CATCTTATGATGAAAAATGGCGATGCTCGTTCGAGTGAATCAAACAAAAATTCTGATAAC
AAATTACAGTCAAATGAGATAAAAAATGTAAATGACTCATCCAAATCACCTTCACCAATA
TCCGAGGCATCAGTAGAGCACCACGCATTAAAGACCGCTGCAGGAGATATGCCAATGAAG
CCCACACCAATGTACTCGAATTTTCCAATTCCTTTTCCACTCGGCATTCATCCTGCCTTT
CATCCAGCTGCCTATTTAAATTATGCAGATGCAATACATAAAGCGTCTTCAGCACGAAAC
ATGTGGCCTTACTTTCATCCATATAGTAGCTATTTAATGCCACCGTGTGGTTCAAAACGC
AAAGGCGGACAGGTTCGTTTTACGCCACAACAGACACAAAATTTGGAGCGACGCTTCTCA
AATCATAAATATTTATCGCCTGAAGATAGACGCAAACTAGCGATGGAATTAAGTCTGAGT
GATAGACAAGTCAAAACGTGGTTCCAGAACAGGAGGTTTTTTCTTGAAATCCTTAAAAAT
ATTCTCAATAATAAGAAGTCTAATCGCAGGGCAAAACTAAAACGTGATATGAGGCATGTC
AAAAAGGATGTCGAGAGTGTCAAGTTGCTAACGGCACACAAGAGCTTCCTGGAGAATGTA
AACGATATGAATTTGCTAAAGTCAAAGGTGACGCACTCACACGTTGATCGTGATGACCAT
CTGAGTTCGGATAAATTTCGAAGTTTAAGTGAGACTGATCAGTAA
>g6281.t2 Gene=g6281 Length=294
MYPFSKNKASFRIADILHQHQQQQQQQQQQTAADNQLLAQHLMMKNGDARSSESNKNSDN
KLQSNEIKNVNDSSKSPSPISEASVEHHALKTAAGDMPMKPTPMYSNFPIPFPLGIHPAF
HPAAYLNYADAIHKASSARNMWPYFHPYSSYLMPPCGSKRKGGQVRFTPQQTQNLERRFS
NHKYLSPEDRRKLAMELSLSDRQVKTWFQNRRFFLEILKNILNNKKSNRRAKLKRDMRHV
KKDVESVKLLTAHKSFLENVNDMNLLKSKVTHSHVDRDDHLSSDKFRSLSETDQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g6281.t2 | CDD | cd00086 | homeodomain | 161 | 212 | 2.00879E-16 |
| 5 | g6281.t2 | Coils | Coil | Coil | 223 | 243 | - |
| 4 | g6281.t2 | Gene3D | G3DSA:1.10.10.60 | - | 167 | 213 | 2.6E-18 |
| 9 | g6281.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 46 | 85 | - |
| 8 | g6281.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 47 | 81 | - |
| 2 | g6281.t2 | PANTHER | PTHR24324 | HOMEOBOX PROTEIN HHEX | 20 | 290 | 9.1E-24 |
| 1 | g6281.t2 | Pfam | PF00046 | Homeodomain | 165 | 212 | 6.4E-18 |
| 10 | g6281.t2 | ProSiteProfiles | PS50071 | ‘Homeobox’ domain profile. | 158 | 212 | 17.265 |
| 7 | g6281.t2 | SMART | SM00389 | HOX_1 | 159 | 222 | 4.4E-16 |
| 3 | g6281.t2 | SUPERFAMILY | SSF46689 | Homeodomain-like | 141 | 212 | 8.13E-19 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003677 | DNA binding | MF |
KEGG
Orthology
- This transcript has been annotated with K08024 (homeobox protein HEX)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
