Gene loci information

Transcript annotation

  • This transcript has been annotated as Enolase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g6296 g6296.t1 TTS g6296.t1 16018227 16018227
chr_2 g6296 g6296.t1 isoform g6296.t1 16018306 16019613
chr_2 g6296 g6296.t1 exon g6296.t1.exon1 16018306 16019613
chr_2 g6296 g6296.t1 cds g6296.t1.CDS1 16018306 16019613
chr_2 g6296 g6296.t1 TSS g6296.t1 16020625 16020625

Sequences

>g6296.t1 Gene=g6296 Length=1308
ATGCCAATCAAAAGCATCAAAGCACGTCAAATTTTTGACTCTCGCGGTAACCCAACCGTT
GAAGTTGATCTCACAACTGAATTGGGAATGTTCCGTGCTGCAGTTCCATCAGGAGCTTCG
ACTGGCATCCATGAAGCACTTGAATTACGTGACAAGGAAGCTGCTAACTGGATGGGAAAG
GGTGTTTTGAAAGCCGTTGCTCATGTCAATGACTCACTCGGCCCAGAAGTTATCAAATCA
GGCTTGGAAGTAACTCAACAACGCGAAATCGATGAATTGATGATCAAACTTGATGGCACA
CCAAACAAATCAAAGTTCGGTGCTAATGCTATTTTGGGTGTCTCATTGGCTGTCTGCAAA
GCTGGTGCAGCAAAGAAAGGTGTTCCTCTTTATCAACATATTGCTGACTTAGCTGGCAAC
AAGGATATCTGGCTTCCAGTTCCAGCTTTTAACGTCATTAACGGTGGTGAACATGCTGGT
AACTTGTTAGCTATGCAAGAATTTATGCTCTTGCCAGTTGGAGCTAAGTCATTCACTGAG
GCAATGAAAATGGGAGCTGAAACATACCACAATTTGGCTAAAGTTATTCAACAAAAGTTC
GGAAAGGATGCCACAGCTGTCGGTGATGAAGGTGGTTTTGCACCAAACATCCTTGATAAT
CGTGCTGGTTTGGAATTGATTGTTGAGGCTATTGAAAAAGCTGGTTACACTGGCAAAATT
AAGATCGGTATGGATGTTGCTGCTTCAGAATTCAAGGACGAAGATGGAACATATGACTTA
TTCAAGAAAGACAAGGTTAAGGATAAGTCACGCAACTTATCATCAGATAAATTGGGTGAT
CTCTATATGGAATTCATCAAGGATTTCCCAGTTGTAAGTATTGAGGACCCATTTGATCAA
GACGATTGGGAAGCATGGACTAAACTTAATGCTCGTGCTGGAATTCAAATTGTCGGTGAT
GATTTGACTGTCACTAATCCAAAACGTATTCAAACCGCTGTCGAAAAGAAGGCTTGCAAC
TGCTTGTTACTCAAAGTCAACCAAATCGGCTCAGTCACAGAGTCAATTGATGCTCATTTG
TTAGCAAAGAAAAATGGCTGGGGTACAATGGTCTCACATCGTTCAGGCGAAACTGAAGAC
TCATTCATTGCTGACTTGGTTGTTGGTCTTTCAACTGGTCAAATTAAGACTGGTGCACCA
TGCCGTTCTGAGAGATTGTGCAAATACAATCAAATCTTACGTATTGAGGAAGAACTCGGA
GCTAATGCCAAGTTTGCTGGCGAGAAATTCAGAAATCCACACGCCTAA

>g6296.t1 Gene=g6296 Length=435
MPIKSIKARQIFDSRGNPTVEVDLTTELGMFRAAVPSGASTGIHEALELRDKEAANWMGK
GVLKAVAHVNDSLGPEVIKSGLEVTQQREIDELMIKLDGTPNKSKFGANAILGVSLAVCK
AGAAKKGVPLYQHIADLAGNKDIWLPVPAFNVINGGEHAGNLLAMQEFMLLPVGAKSFTE
AMKMGAETYHNLAKVIQQKFGKDATAVGDEGGFAPNILDNRAGLELIVEAIEKAGYTGKI
KIGMDVAASEFKDEDGTYDLFKKDKVKDKSRNLSSDKLGDLYMEFIKDFPVVSIEDPFDQ
DDWEAWTKLNARAGIQIVGDDLTVTNPKRIQTAVEKKACNCLLLKVNQIGSVTESIDAHL
LAKKNGWGTMVSHRSGETEDSFIADLVVGLSTGQIKTGAPCRSERLCKYNQILRIEEELG
ANAKFAGEKFRNPHA

Protein features from InterProScan

Transcript Database ID Name Start End E.value
16 g6296.t1 CDD cd03313 enolase 5 418 0.0
14 g6296.t1 Gene3D G3DSA:3.30.390.10 - 1 127 3.1E-59
13 g6296.t1 Gene3D G3DSA:3.20.20.120 Enolase superfamily 128 435 1.8E-143
4 g6296.t1 Hamap MF_00318 Enolase [eno]. 1 419 42.620113
3 g6296.t1 PANTHER PTHR11902 ENOLASE 1 432 2.9E-225
15 g6296.t1 PIRSF PIRSF001400 Enolase 1 432 1.6E-184
6 g6296.t1 PRINTS PR00148 Enolase signature 35 49 9.0E-54
7 g6296.t1 PRINTS PR00148 Enolase signature 107 123 9.0E-54
10 g6296.t1 PRINTS PR00148 Enolase signature 164 177 9.0E-54
8 g6296.t1 PRINTS PR00148 Enolase signature 319 330 9.0E-54
5 g6296.t1 PRINTS PR00148 Enolase signature 342 356 9.0E-54
9 g6296.t1 PRINTS PR00148 Enolase signature 371 388 9.0E-54
1 g6296.t1 Pfam PF03952 Enolase, N-terminal domain 3 134 8.5E-57
2 g6296.t1 Pfam PF00113 Enolase, C-terminal TIM barrel domain 144 432 9.9E-147
19 g6296.t1 ProSitePatterns PS00164 Enolase signature. 342 355 -
21 g6296.t1 SFLD SFLDG00178 enolase 3 419 0.0
22 g6296.t1 SFLD SFLDF00002 enolase 3 419 0.0
17 g6296.t1 SMART SM01193 Enolase_N_3 3 134 3.3E-91
18 g6296.t1 SMART SM01192 Enolase_C_3 142 433 3.6E-192
11 g6296.t1 SUPERFAMILY SSF54826 Enolase N-terminal domain-like 3 138 7.29E-56
12 g6296.t1 SUPERFAMILY SSF51604 Enolase C-terminal domain-like 143 432 2.18E-125
20 g6296.t1 TIGRFAM TIGR01060 eno: phosphopyruvate hydratase 3 430 1.3E-165

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0000287 magnesium ion binding MF
GO:0004634 phosphopyruvate hydratase activity MF
GO:0006096 glycolytic process BP
GO:0000015 phosphopyruvate hydratase complex CC

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values