Gene loci information

Transcript annotation

  • This transcript has been annotated as Enolase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g6296 g6296.t5 isoform g6296.t5 16017843 16020627
chr_2 g6296 g6296.t5 exon g6296.t5.exon1 16017843 16018812
chr_2 g6296 g6296.t5 TTS g6296.t5 16017865 16017865
chr_2 g6296 g6296.t5 cds g6296.t5.CDS1 16018808 16018812
chr_2 g6296 g6296.t5 exon g6296.t5.exon2 16018887 16019620
chr_2 g6296 g6296.t5 cds g6296.t5.CDS2 16018887 16019613
chr_2 g6296 g6296.t5 exon g6296.t5.exon3 16020562 16020627
chr_2 g6296 g6296.t5 TSS g6296.t5 16020625 16020625

Sequences

>g6296.t5 Gene=g6296 Length=1770
ATTTTCAAGCTGAATCGCGTTGAGAAAGTTCACTTGTAGCCGATTTCCTGAAAATTAATT
GAAAAACTCAAAAATGCCAATCAAAAGCATCAAAGCACGTCAAATTTTTGACTCTCGCGG
TAACCCAACCGTTGAAGTTGATCTCACAACTGAATTGGGAATGTTCCGTGCTGCAGTTCC
ATCAGGAGCTTCGACTGGCATCCATGAAGCACTTGAATTACGTGACAAGGAAGCTGCTAA
CTGGATGGGAAAGGGTGTTTTGAAAGCCGTTGCTCATGTCAATGACTCACTCGGCCCAGA
AGTTATCAAATCAGGCTTGGAAGTAACTCAACAACGCGAAATCGATGAATTGATGATCAA
ACTTGATGGCACACCAAACAAATCAAAGTTCGGTGCTAATGCTATTTTGGGTGTCTCATT
GGCTGTCTGCAAAGCTGGTGCAGCAAAGAAAGGTGTTCCTCTTTATCAACATATTGCTGA
CTTAGCTGGCAACAAGGATATCTGGCTTCCAGTTCCAGCTTTTAACGTCATTAACGGTGG
TGAACATGCTGGTAACTTGTTAGCTATGCAAGAATTTATGCTCTTGCCAGTTGGAGCTAA
GTCATTCACTGAGGCAATGAAAATGGGAGCTGAAACATACCACAATTTGGCTAAAGTTAT
TCAACAAAAGTTCGGAAAGGATGCCACAGCTGTCGGTGATGAAGGTGGTTTTGCACCAAA
CATCCTTGATAATCGTGCTGGTTTGGAATTGATTGTTGAGGCTATTGAAAAAGCTGGTTA
CACTGGCAAAATTAAGATCGGATAAGTCACGCAACTTATCATCAGATAAATTGGGTGATC
TCTATATGGAATTCATCAAGGATTTCCCAGTTGTAAGTATTGAGGACCCATTTGATCAAG
ACGATTGGGAAGCATGGACTAAACTTAATGCTCGTGCTGGAATTCAAATTGTCGGTGATG
ATTTGACTGTCACTAATCCAAAACGTATTCAAACCGCTGTCGAAAAGAAGGCTTGCAACT
GCTTGTTACTCAAAGTCAACCAAATCGGCTCAGTCACAGAGTCAATTGATGCTCATTTGT
TAGCAAAGAAAAATGGCTGGGGTACAATGGTCTCACATCGTTCAGGCGAAACTGAAGACT
CATTCATTGCTGACTTGGTTGTTGGTCTTTCAACTGGTCAAATTAAGACTGGTGCACCAT
GCCGTTCTGAGAGATTGTGCAAATACAATCAAATCTTACGTATTGAGGAAGAACTCGGAG
CTAATGCCAAGTTTGCTGGCGAGAAATTCAGAAATCCACACGCCTAAATTTTCAGCTGAA
TTTATTTCTCTTATTCTAATTTAATGCAACACAAAACATACAAAAATAAAGTTAATTGAT
AAAAACATCCCATATGTCTATACTAGACCGAAATAATTCTGCATTTCCTCTTGATTTCTC
CTCCTTACATCGATGACTATCCATATCTTTATTAGAGATGCTCTGCTATGTATGCTATAA
GTGAGGAGAAAGCAGACAGACATATGATTAATATCACATCACACAAGTATAACTACTATA
TTCATGATGTTAATCTCAAATTATCTAGTTAAGGATATTTTAAATATCTGTCTCTCATTG
AAAAAAGTTTCCTTAATAAAAGAAGACGTGAAAAGTTTTAAAGACACGCACTCATAATAA
AATGAAATGTAGGCTTCTTGATTTGTTGTTAATAAAGAAGAAAAATAAAAAAGATGTTCA
ATGAATATAAAAATAGAATTTTGTTTTTAT

>g6296.t5 Gene=g6296 Length=243
MPIKSIKARQIFDSRGNPTVEVDLTTELGMFRAAVPSGASTGIHEALELRDKEAANWMGK
GVLKAVAHVNDSLGPEVIKSGLEVTQQREIDELMIKLDGTPNKSKFGANAILGVSLAVCK
AGAAKKGVPLYQHIADLAGNKDIWLPVPAFNVINGGEHAGNLLAMQEFMLLPVGAKSFTE
AMKMGAETYHNLAKVIQQKFGKDATAVGDEGGFAPNILDNRAGLELIVEAIEKAGYTGKI
KIG

Protein features from InterProScan

Transcript Database ID Name Start End E.value
13 g6296.t5 Gene3D G3DSA:3.30.390.10 - 1 127 0
12 g6296.t5 Gene3D G3DSA:3.20.20.120 Enolase superfamily 128 243 0
3 g6296.t5 PANTHER PTHR11902:SF5 BETA-ENOLASE 1 243 0
4 g6296.t5 PANTHER PTHR11902 ENOLASE 1 243 0
5 g6296.t5 PRINTS PR00148 Enolase signature 35 49 0
6 g6296.t5 PRINTS PR00148 Enolase signature 107 123 0
7 g6296.t5 PRINTS PR00148 Enolase signature 164 177 0
1 g6296.t5 Pfam PF03952 Enolase, N-terminal domain 3 134 0
2 g6296.t5 Pfam PF00113 Enolase, C-terminal TIM barrel domain 144 243 0
10 g6296.t5 SMART SM01193 Enolase_N_3 3 134 0
11 g6296.t5 SMART SM01192 Enolase_C_3 142 243 0
8 g6296.t5 SUPERFAMILY SSF54826 Enolase N-terminal domain-like 3 138 0
9 g6296.t5 SUPERFAMILY SSF51604 Enolase C-terminal domain-like 143 243 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0000287 magnesium ion binding MF
GO:0004634 phosphopyruvate hydratase activity MF
GO:0006096 glycolytic process BP
GO:0000015 phosphopyruvate hydratase complex CC

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values