Gene loci information

Transcript annotation

  • This transcript has been annotated as Enolase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g6296 g6296.t6 TTS g6296.t6 16017865 16017865
chr_2 g6296 g6296.t6 isoform g6296.t6 16018227 16019616
chr_2 g6296 g6296.t6 exon g6296.t6.exon1 16018227 16019307
chr_2 g6296 g6296.t6 cds g6296.t6.CDS1 16018306 16019307
chr_2 g6296 g6296.t6 exon g6296.t6.exon2 16019410 16019616
chr_2 g6296 g6296.t6 cds g6296.t6.CDS2 16019410 16019613
chr_2 g6296 g6296.t6 TSS g6296.t6 16020625 16020625

Sequences

>g6296.t6 Gene=g6296 Length=1288
AAAATGCCAATCAAAAGCATCAAAGCACGTCAAATTTTTGACTCTCGCGGTAACCCAACC
GTTGAAGTTGATCTCACAACTGAATTGGGAATGTTCCGTGCTGCAGTTCCATCAGGAGCT
TCGACTGGCATCCATGAAGCACTTGAATTACGTGACAAGGAAGCTGCTAACTGGATGGGA
AAGGGTGTTTTGAAAGCCGTTGCTCATAAATCAAAGTTCGGTGCTAATGCTATTTTGGGT
GTCTCATTGGCTGTCTGCAAAGCTGGTGCAGCAAAGAAAGGTGTTCCTCTTTATCAACAT
ATTGCTGACTTAGCTGGCAACAAGGATATCTGGCTTCCAGTTCCAGCTTTTAACGTCATT
AACGGTGGTGAACATGCTGGTAACTTGTTAGCTATGCAAGAATTTATGCTCTTGCCAGTT
GGAGCTAAGTCATTCACTGAGGCAATGAAAATGGGAGCTGAAACATACCACAATTTGGCT
AAAGTTATTCAACAAAAGTTCGGAAAGGATGCCACAGCTGTCGGTGATGAAGGTGGTTTT
GCACCAAACATCCTTGATAATCGTGCTGGTTTGGAATTGATTGTTGAGGCTATTGAAAAA
GCTGGTTACACTGGCAAAATTAAGATCGGTATGGATGTTGCTGCTTCAGAATTCAAGGAC
GAAGATGGAACATATGACTTATTCAAGAAAGACAAGGTTAAGGATAAGTCACGCAACTTA
TCATCAGATAAATTGGGTGATCTCTATATGGAATTCATCAAGGATTTCCCAGTTGTAAGT
ATTGAGGACCCATTTGATCAAGACGATTGGGAAGCATGGACTAAACTTAATGCTCGTGCT
GGAATTCAAATTGTCGGTGATGATTTGACTGTCACTAATCCAAAACGTATTCAAACCGCT
GTCGAAAAGAAGGCTTGCAACTGCTTGTTACTCAAAGTCAACCAAATCGGCTCAGTCACA
GAGTCAATTGATGCTCATTTGTTAGCAAAGAAAAATGGCTGGGGTACAATGGTCTCACAT
CGTTCAGGCGAAACTGAAGACTCATTCATTGCTGACTTGGTTGTTGGTCTTTCAACTGGT
CAAATTAAGACTGGTGCACCATGCCGTTCTGAGAGATTGTGCAAATACAATCAAATCTTA
CGTATTGAGGAAGAACTCGGAGCTAATGCCAAGTTTGCTGGCGAGAAATTCAGAAATCCA
CACGCCTAAATTTTCAGCTGAATTTATTTCTCTTATTCTAATTTAATGCAACACAAAACA
TACAAAAATAAAGTTAATTGATAAAAAC

>g6296.t6 Gene=g6296 Length=401
MPIKSIKARQIFDSRGNPTVEVDLTTELGMFRAAVPSGASTGIHEALELRDKEAANWMGK
GVLKAVAHKSKFGANAILGVSLAVCKAGAAKKGVPLYQHIADLAGNKDIWLPVPAFNVIN
GGEHAGNLLAMQEFMLLPVGAKSFTEAMKMGAETYHNLAKVIQQKFGKDATAVGDEGGFA
PNILDNRAGLELIVEAIEKAGYTGKIKIGMDVAASEFKDEDGTYDLFKKDKVKDKSRNLS
SDKLGDLYMEFIKDFPVVSIEDPFDQDDWEAWTKLNARAGIQIVGDDLTVTNPKRIQTAV
EKKACNCLLLKVNQIGSVTESIDAHLLAKKNGWGTMVSHRSGETEDSFIADLVVGLSTGQ
IKTGAPCRSERLCKYNQILRIEEELGANAKFAGEKFRNPHA

Protein features from InterProScan

Transcript Database ID Name Start End E.value
17 g6296.t6 CDD cd03313 enolase 5 384 0.0
13 g6296.t6 Gene3D G3DSA:3.30.390.10 - 1 67 1.5E-28
14 g6296.t6 Gene3D G3DSA:3.30.390.10 - 68 93 7.3E-7
12 g6296.t6 Gene3D G3DSA:3.20.20.120 Enolase superfamily 94 401 1.4E-143
5 g6296.t6 Hamap MF_00318 Enolase [eno]. 1 385 37.793961
4 g6296.t6 PANTHER PTHR11902 ENOLASE 1 68 3.1E-206
3 g6296.t6 PANTHER PTHR11902 ENOLASE 69 398 3.1E-206
16 g6296.t6 PIRSF PIRSF001400 Enolase 1 68 3.6E-25
15 g6296.t6 PIRSF PIRSF001400 Enolase 67 398 7.8E-141
7 g6296.t6 PRINTS PR00148 Enolase signature 35 49 9.4E-33
8 g6296.t6 PRINTS PR00148 Enolase signature 285 296 9.4E-33
6 g6296.t6 PRINTS PR00148 Enolase signature 308 322 9.4E-33
9 g6296.t6 PRINTS PR00148 Enolase signature 337 354 9.4E-33
1 g6296.t6 Pfam PF03952 Enolase, N-terminal domain 3 68 4.5E-25
2 g6296.t6 Pfam PF00113 Enolase, C-terminal TIM barrel domain 110 398 7.7E-147
20 g6296.t6 ProSitePatterns PS00164 Enolase signature. 308 321 -
21 g6296.t6 SFLD SFLDG00178 enolase 68 385 0.0
22 g6296.t6 SFLD SFLDS00001 Enolase 68 385 0.0
18 g6296.t6 SMART SM01193 Enolase_N_3 3 100 1.9E-55
19 g6296.t6 SMART SM01192 Enolase_C_3 108 399 3.6E-192
10 g6296.t6 SUPERFAMILY SSF54826 Enolase N-terminal domain-like 2 105 9.28E-39
11 g6296.t6 SUPERFAMILY SSF51604 Enolase C-terminal domain-like 109 398 1.63E-125

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0000287 magnesium ion binding MF
GO:0004634 phosphopyruvate hydratase activity MF
GO:0006096 glycolytic process BP
GO:0000015 phosphopyruvate hydratase complex CC

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values