Gene loci information

Transcript annotation

  • This transcript has been annotated as Enolase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g6296 g6296.t8 TTS g6296.t8 16017865 16017865
chr_2 g6296 g6296.t8 isoform g6296.t8 16018227 16020627
chr_2 g6296 g6296.t8 exon g6296.t8.exon1 16018227 16019372
chr_2 g6296 g6296.t8 cds g6296.t8.CDS1 16018306 16019372
chr_2 g6296 g6296.t8 exon g6296.t8.exon2 16019568 16019620
chr_2 g6296 g6296.t8 cds g6296.t8.CDS2 16019568 16019613
chr_2 g6296 g6296.t8 exon g6296.t8.exon3 16020562 16020627
chr_2 g6296 g6296.t8 TSS g6296.t8 16020625 16020625

Sequences

>g6296.t8 Gene=g6296 Length=1265
ATTTTCAAGCTGAATCGCGTTGAGAAAGTTCACTTGTAGCCGATTTCCTGAAAATTAATT
GAAAAACTCAAAAATGCCAATCAAAAGCATCAAAGCACGTCAAATTTTTGACTCTCGCGG
CTTGGAAGTAACTCAACAACGCGAAATCGATGAATTGATGATCAAACTTGATGGCACACC
AAACAAATCAAAGTTCGGTGCTAATGCTATTTTGGGTGTCTCATTGGCTGTCTGCAAAGC
TGGTGCAGCAAAGAAAGGTGTTCCTCTTTATCAACATATTGCTGACTTAGCTGGCAACAA
GGATATCTGGCTTCCAGTTCCAGCTTTTAACGTCATTAACGGTGGTGAACATGCTGGTAA
CTTGTTAGCTATGCAAGAATTTATGCTCTTGCCAGTTGGAGCTAAGTCATTCACTGAGGC
AATGAAAATGGGAGCTGAAACATACCACAATTTGGCTAAAGTTATTCAACAAAAGTTCGG
AAAGGATGCCACAGCTGTCGGTGATGAAGGTGGTTTTGCACCAAACATCCTTGATAATCG
TGCTGGTTTGGAATTGATTGTTGAGGCTATTGAAAAAGCTGGTTACACTGGCAAAATTAA
GATCGGTATGGATGTTGCTGCTTCAGAATTCAAGGACGAAGATGGAACATATGACTTATT
CAAGAAAGACAAGGTTAAGGATAAGTCACGCAACTTATCATCAGATAAATTGGGTGATCT
CTATATGGAATTCATCAAGGATTTCCCAGTTGTAAGTATTGAGGACCCATTTGATCAAGA
CGATTGGGAAGCATGGACTAAACTTAATGCTCGTGCTGGAATTCAAATTGTCGGTGATGA
TTTGACTGTCACTAATCCAAAACGTATTCAAACCGCTGTCGAAAAGAAGGCTTGCAACTG
CTTGTTACTCAAAGTCAACCAAATCGGCTCAGTCACAGAGTCAATTGATGCTCATTTGTT
AGCAAAGAAAAATGGCTGGGGTACAATGGTCTCACATCGTTCAGGCGAAACTGAAGACTC
ATTCATTGCTGACTTGGTTGTTGGTCTTTCAACTGGTCAAATTAAGACTGGTGCACCATG
CCGTTCTGAGAGATTGTGCAAATACAATCAAATCTTACGTATTGAGGAAGAACTCGGAGC
TAATGCCAAGTTTGCTGGCGAGAAATTCAGAAATCCACACGCCTAAATTTTCAGCTGAAT
TTATTTCTCTTATTCTAATTTAATGCAACACAAAACATACAAAAATAAAGTTAATTGATA
AAAAC

>g6296.t8 Gene=g6296 Length=370
MPIKSIKARQIFDSRGLEVTQQREIDELMIKLDGTPNKSKFGANAILGVSLAVCKAGAAK
KGVPLYQHIADLAGNKDIWLPVPAFNVINGGEHAGNLLAMQEFMLLPVGAKSFTEAMKMG
AETYHNLAKVIQQKFGKDATAVGDEGGFAPNILDNRAGLELIVEAIEKAGYTGKIKIGMD
VAASEFKDEDGTYDLFKKDKVKDKSRNLSSDKLGDLYMEFIKDFPVVSIEDPFDQDDWEA
WTKLNARAGIQIVGDDLTVTNPKRIQTAVEKKACNCLLLKVNQIGSVTESIDAHLLAKKN
GWGTMVSHRSGETEDSFIADLVVGLSTGQIKTGAPCRSERLCKYNQILRIEEELGANAKF
AGEKFRNPHA

Protein features from InterProScan

Transcript Database ID Name Start End E.value
14 g6296.t8 CDD cd03313 enolase 16 353 0.0
13 g6296.t8 Gene3D G3DSA:3.30.390.10 - 16 62 2.3E-19
12 g6296.t8 Gene3D G3DSA:3.20.20.120 Enolase superfamily 63 370 1.1E-143
4 g6296.t8 Hamap MF_00318 Enolase [eno]. 1 354 33.13372
3 g6296.t8 PANTHER PTHR11902 ENOLASE 18 367 5.2E-192
7 g6296.t8 PRINTS PR00148 Enolase signature 42 58 6.7E-44
6 g6296.t8 PRINTS PR00148 Enolase signature 99 112 6.7E-44
8 g6296.t8 PRINTS PR00148 Enolase signature 254 265 6.7E-44
5 g6296.t8 PRINTS PR00148 Enolase signature 277 291 6.7E-44
9 g6296.t8 PRINTS PR00148 Enolase signature 306 323 6.7E-44
1 g6296.t8 Pfam PF03952 Enolase, N-terminal domain 16 69 1.0E-21
2 g6296.t8 Pfam PF00113 Enolase, C-terminal TIM barrel domain 79 367 5.9E-147
17 g6296.t8 ProSitePatterns PS00164 Enolase signature. 277 290 -
19 g6296.t8 SFLD SFLDG00178 enolase 16 354 0.0
20 g6296.t8 SFLD SFLDS00001 Enolase 16 354 0.0
15 g6296.t8 SMART SM01193 Enolase_N_3 3 69 4.7E-18
16 g6296.t8 SMART SM01192 Enolase_C_3 77 368 3.6E-192
10 g6296.t8 SUPERFAMILY SSF54826 Enolase N-terminal domain-like 3 75 3.31E-23
11 g6296.t8 SUPERFAMILY SSF51604 Enolase C-terminal domain-like 78 367 1.28E-125
18 g6296.t8 TIGRFAM TIGR01060 eno: phosphopyruvate hydratase 15 365 6.1E-134

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0000287 magnesium ion binding MF
GO:0004634 phosphopyruvate hydratase activity MF
GO:0006096 glycolytic process BP
GO:0000015 phosphopyruvate hydratase complex CC

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values