General information for g63.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 6A1.
Parent gene
- The parent gene g63 is located on chr_3: 447236..450989 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g63 | g63.t1 | TTS | g63.t1 | 446108 | 446108 |
| chr_3 | g63 | g63.t1 | isoform | g63.t1 | 446462 | 448301 |
| chr_3 | g63 | g63.t1 | exon | g63.t1.exon1 | 446462 | 446635 |
| chr_3 | g63 | g63.t1 | cds | g63.t1.CDS1 | 446462 | 446635 |
| chr_3 | g63 | g63.t1 | exon | g63.t1.exon2 | 446695 | 447107 |
| chr_3 | g63 | g63.t1 | cds | g63.t1.CDS2 | 446695 | 447107 |
| chr_3 | g63 | g63.t1 | exon | g63.t1.exon3 | 447169 | 447510 |
| chr_3 | g63 | g63.t1 | cds | g63.t1.CDS3 | 447169 | 447510 |
| chr_3 | g63 | g63.t1 | exon | g63.t1.exon4 | 447599 | 447815 |
| chr_3 | g63 | g63.t1 | cds | g63.t1.CDS4 | 447599 | 447815 |
| chr_3 | g63 | g63.t1 | exon | g63.t1.exon5 | 447939 | 448301 |
| chr_3 | g63 | g63.t1 | cds | g63.t1.CDS5 | 447939 | 448301 |
| chr_3 | g63 | g63.t1 | TSS | g63.t1 | 448395 | 448395 |
Sequences
>g63.t1 Gene=g63 Length=1509
ATGGCGTCGTCATTAGTTCTCATAAGCATTAGTCTCTTATTTTTATATCTTTTTAATTTA
GTTAAAAAACAATACAATTATTTTAAAGATCGTGGAATACCAAACCCAAAGCCAATTTTC
CCATTAGGCAATTTTTGGGGTGTTGGATTATCTGTGCATTTTATTCATCGTGTAAATGAA
ATTTATAGACAATTTAAAGGCAAAGCAAAAATTTGTGGATTTTTTGTCACTACAATACCA
GCATATATCGCACTTGATATTGAATTGATCAAGAATATTCTAGTGAAAGATTTTGACTCA
TTTCATGATCGTGGAATCTATTATAATGAAAAAGCCGATCCCTTATCAGCGCATATCTTT
TCGATAGCGGGTGAGCAATGGAAAACATTACGACAAAAAATGACTACGACATTTTCGAGT
GGCAAAATTAAAGCTATGATGCCGACCTTAAATAAAATTTCTGAAGAAATGACGGAAGTA
CTTGGTGAATATGCAGATGAAGGCAAAACTTTGAATTATAGAGACATGGCATCACGTCTT
ATGTGCGAAATTGTAGGAGAGATTGCATTTGGAATTAAATGTAATGCACTTCGTGATCCC
AATTGTGAAATGATGGTATTAAGTGATTTTCTCAGTATTAAAGACATGAAACAAAGAGTT
ATGTATTTATTTTCAAACGCATTTCCTGAAGTATGTCGTAAATTAAATATTCAAATCACA
CCCAAAGATGTACAACATTATTTTCTTAAAATTATCAACGAAGCAATTGAATATCGTGAA
AAGAACAAAATACGTAGAAATGATTATTTTGACATGCTTCTTCAGTTGAAAAATGAAGGA
AAACTTGATGGTGATGTGACAAATGTTGGAAGAATTTCACACACAGACATGTTGGCAGCA
TGCTTTGTCTTTTTTGTAGCTGGCTTCGAAACTTCATCAACTGCACTCGCTTATGCTCTC
TATGAACTTGCAGTAAATCAAGAACTTCAAGATCGCACACGTGATGAAATTGAAAAAGTT
TTAAGTAATTATGATGGAAAAGTGACATATGAAGCTCTCATGAATATGAATTATCCTGGT
CAAGTTATTAGTGAATCACTTCGTAAATACACACCAGGAAATGTTTTGATGAGAAAATGT
ACAAAAGAATATAAAATTCCTGACACTGATATTGTAATTGAGAAAGGTCGAATGGTCTTT
TTACCAATGCATGCCATTCATAATGATCCTGAGTACTTCCCTGATCCTGAAAAATTTGAT
CCTGATAGATTTTCTCCTGAAATGGAAAAAGAACGAAATCCATTTACATTTTTACCATTT
GGTGAAGGAATGAGAATCTGCATGGGTCTACGTTTCGGTCTAATTCAAACACGTCTTGGC
CTTGTTTCGGTTTTACGTAAATACCGTGTAACACTCAATGAGAAAACAGTTCATCCTATT
CGTCTTGATCCAAAAGATGTCACAATGATGCCTTACGGTGGTATTTGGCTCAACACAGAA
CACATCTGA
>g63.t1 Gene=g63 Length=502
MASSLVLISISLLFLYLFNLVKKQYNYFKDRGIPNPKPIFPLGNFWGVGLSVHFIHRVNE
IYRQFKGKAKICGFFVTTIPAYIALDIELIKNILVKDFDSFHDRGIYYNEKADPLSAHIF
SIAGEQWKTLRQKMTTTFSSGKIKAMMPTLNKISEEMTEVLGEYADEGKTLNYRDMASRL
MCEIVGEIAFGIKCNALRDPNCEMMVLSDFLSIKDMKQRVMYLFSNAFPEVCRKLNIQIT
PKDVQHYFLKIINEAIEYREKNKIRRNDYFDMLLQLKNEGKLDGDVTNVGRISHTDMLAA
CFVFFVAGFETSSTALAYALYELAVNQELQDRTRDEIEKVLSNYDGKVTYEALMNMNYPG
QVISESLRKYTPGNVLMRKCTKEYKIPDTDIVIEKGRMVFLPMHAIHNDPEYFPDPEKFD
PDRFSPEMEKERNPFTFLPFGEGMRICMGLRFGLIQTRLGLVSVLRKYRVTLNEKTVHPI
RLDPKDVTMMPYGGIWLNTEHI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g63.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 16 | 502 | 1.5E-123 |
| 2 | g63.t1 | PANTHER | PTHR24292 | CYTOCHROME P450 | 10 | 501 | 6.3E-174 |
| 3 | g63.t1 | PANTHER | PTHR24292:SF95 | CYP6A16, ISOFORM B-RELATED | 10 | 501 | 6.3E-174 |
| 7 | g63.t1 | PRINTS | PR00463 | E-class P450 group I signature | 89 | 110 | 3.0E-20 |
| 4 | g63.t1 | PRINTS | PR00463 | E-class P450 group I signature | 296 | 313 | 3.0E-20 |
| 13 | g63.t1 | PRINTS | PR00385 | P450 superfamily signature | 307 | 324 | 1.1E-9 |
| 8 | g63.t1 | PRINTS | PR00463 | E-class P450 group I signature | 316 | 342 | 3.0E-20 |
| 12 | g63.t1 | PRINTS | PR00385 | P450 superfamily signature | 361 | 372 | 1.1E-9 |
| 9 | g63.t1 | PRINTS | PR00463 | E-class P450 group I signature | 402 | 426 | 3.0E-20 |
| 5 | g63.t1 | PRINTS | PR00463 | E-class P450 group I signature | 437 | 447 | 3.0E-20 |
| 11 | g63.t1 | PRINTS | PR00385 | P450 superfamily signature | 438 | 447 | 1.1E-9 |
| 6 | g63.t1 | PRINTS | PR00463 | E-class P450 group I signature | 447 | 470 | 3.0E-20 |
| 10 | g63.t1 | PRINTS | PR00385 | P450 superfamily signature | 447 | 458 | 1.1E-9 |
| 1 | g63.t1 | Pfam | PF00067 | Cytochrome P450 | 44 | 495 | 2.1E-90 |
| 18 | g63.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 23 | - |
| 19 | g63.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 20 | g63.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 18 | - |
| 21 | g63.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 19 | 23 | - |
| 17 | g63.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 24 | 502 | - |
| 24 | g63.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 440 | 449 | - |
| 14 | g63.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 33 | 498 | 1.07E-105 |
| 15 | g63.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 23 | - |
| 23 | g63.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 4 | 21 | - |
| 22 | g63.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 71 | 90 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed