General information for g635.t16
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial .
Parent gene
- The parent gene g635 is located on chr_3: 4682651..4683238 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g635 | g635.t16 | TSS | g635.t16 | 4679520 | 4679520 |
| chr_3 | g635 | g635.t16 | isoform | g635.t16 | 4680132 | 4682054 |
| chr_3 | g635 | g635.t16 | exon | g635.t16.exon1 | 4680132 | 4680255 |
| chr_3 | g635 | g635.t16 | cds | g635.t16.CDS1 | 4680149 | 4680255 |
| chr_3 | g635 | g635.t16 | exon | g635.t16.exon2 | 4680321 | 4680606 |
| chr_3 | g635 | g635.t16 | cds | g635.t16.CDS2 | 4680321 | 4680606 |
| chr_3 | g635 | g635.t16 | exon | g635.t16.exon3 | 4681640 | 4682054 |
| chr_3 | g635 | g635.t16 | cds | g635.t16.CDS3 | 4681640 | 4682053 |
| chr_3 | g635 | g635.t16 | TTS | g635.t16 | 4682871 | 4682871 |
Sequences
>g635.t16 Gene=g635 Length=825
TTAAAAATAGATTAGTTATGCAGCAACAACATGTTAGATTAATTCAAACAACTACGCAAC
TTCTTAATTCTGAAATCGTTAAGGTGCCACCATTTGCCGATTCTGTATCAGAAGGTGATG
TAAAATTTGATAAGAAAGTAGGTGATGCAGTTGCAGCTGATGAGGTTGTTATGGAAATTG
AAACCGATAAGACAACAGTTGGTGTTCCAGCTCCATCGCATGGTATCATTGAGGAAATTT
TTGTTTCAGACGGTGATACAGTAAAGGCTGGACAACAGTTGTTCAAAATGAAGTTTACAG
ATGCCGCACCAGAAAAAGAAGCATCAACTTCCACGCCAGAACCTCCAAAAGCAGCTGCTG
CACCTTCACCACCACCACCACAACCTAAACAAGAAGCACCAAAAGCTGCTCCTCAACCAC
AAGCAGCACCACCACCTGCTGCATCAACACCTCCACCACCGCCTCCAAAACCAGCAGCAC
CAGCAAAAACCGTTCCAATTGCTGCCATTCGACATGCACAAGCTATCGATGCTGCCACAG
TTAAAGTACCTCCAGCTGATTATACAAAAGAAATCACTGGCACACGCACGGAACAGCGTG
TAAAAATGAATCGAATGCGTCTAAAGATTGCATCACGTTTGAAGGAAGCACAAAATGTCA
ATGCAATGTTAACAACATTCAATGAAATTGATATGAGTGCAATCATGGAATTCCGCAAGT
TGCATCAAGAAACCTTCCAAAAGCGTTATGGAATTAAATTAGGATTTATGTCAGCTTTCT
GTAAAGCTGCTGCTTATGCTCTTCAAGATCAACCAGTTGTTAATG
>g635.t16 Gene=g635 Length=269
MQQQHVRLIQTTTQLLNSEIVKVPPFADSVSEGDVKFDKKVGDAVAADEVVMEIETDKTT
VGVPAPSHGIIEEIFVSDGDTVKAGQQLFKMKFTDAAPEKEASTSTPEPPKAAAAPSPPP
PQPKQEAPKAAPQPQAAPPPAASTPPPPPPKPAAPAKTVPIAAIRHAQAIDAATVKVPPA
DYTKEITGTRTEQRVKMNRMRLKIASRLKEAQNVNAMLTTFNEIDMSAIMEFRKLHQETF
QKRYGIKLGFMSAFCKAAAYALQDQPVVN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g635.t16 | CDD | cd06849 | lipoyl_domain | 20 | 91 | 1.99402E-21 |
| 7 | g635.t16 | Gene3D | G3DSA:2.40.50.100 | - | 16 | 117 | 3.0E-18 |
| 8 | g635.t16 | Gene3D | G3DSA:3.30.559.10 | Chloramphenicol Acetyltransferase | 189 | 269 | 5.1E-19 |
| 12 | g635.t16 | MobiDBLite | mobidb-lite | consensus disorder prediction | 95 | 156 | - |
| 11 | g635.t16 | MobiDBLite | mobidb-lite | consensus disorder prediction | 111 | 156 | - |
| 3 | g635.t16 | PANTHER | PTHR43416 | DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED | 6 | 269 | 3.9E-61 |
| 4 | g635.t16 | PANTHER | PTHR43416:SF18 | DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL | 6 | 269 | 3.9E-61 |
| 1 | g635.t16 | Pfam | PF00364 | Biotin-requiring enzyme | 21 | 91 | 5.0E-18 |
| 2 | g635.t16 | Pfam | PF00198 | 2-oxoacid dehydrogenases acyltransferase (catalytic domain) | 191 | 269 | 1.6E-13 |
| 10 | g635.t16 | ProSitePatterns | PS00189 | 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site. | 42 | 71 | - |
| 13 | g635.t16 | ProSiteProfiles | PS50968 | Biotinyl/lipoyl domain profile. | 18 | 92 | 24.11 |
| 5 | g635.t16 | SUPERFAMILY | SSF51230 | Single hybrid motif | 19 | 110 | 6.15E-20 |
| 6 | g635.t16 | SUPERFAMILY | SSF52777 | CoA-dependent acyltransferases | 180 | 269 | 4.89E-18 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016746 | acyltransferase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
