Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g635 g635.t7 TSS g635.t7 4679520 4679520
chr_3 g635 g635.t7 isoform g635.t7 4679597 4682070
chr_3 g635 g635.t7 exon g635.t7.exon1 4679597 4679662
chr_3 g635 g635.t7 cds g635.t7.CDS1 4679597 4679662
chr_3 g635 g635.t7 exon g635.t7.exon2 4679983 4680068
chr_3 g635 g635.t7 cds g635.t7.CDS2 4679983 4680068
chr_3 g635 g635.t7 exon g635.t7.exon3 4680127 4680255
chr_3 g635 g635.t7 cds g635.t7.CDS3 4680127 4680255
chr_3 g635 g635.t7 exon g635.t7.exon4 4680321 4680606
chr_3 g635 g635.t7 cds g635.t7.CDS4 4680321 4680606
chr_3 g635 g635.t7 exon g635.t7.exon5 4681640 4682070
chr_3 g635 g635.t7 cds g635.t7.CDS5 4681640 4682068
chr_3 g635 g635.t7 TTS g635.t7 4682871 4682871

Sequences

>g635.t7 Gene=g635 Length=998
ATGATTCGATCACTTTCCTCATTTCCACGAAGTGTTGTTCTTCGCATAAGCAATAGAAGT
AATCAGATCCAGCAAACGTTGAGGAATAATATTCATCAACAATGTGCCGTTCAAAAGTTG
GTGCTTACATGCGACACAAATTCTCAAAATTCAACACTTAAAAATAGATTAGTTATGCAG
CAACAACATGTTAGATTAATTCAAACAACTACGCAACTTCTTAATTCTGAAATCGTTAAG
GTGCCACCATTTGCCGATTCTGTATCAGAAGGTGATGTAAAATTTGATAAGAAAGTAGGT
GATGCAGTTGCAGCTGATGAGGTTGTTATGGAAATTGAAACCGATAAGACAACAGTTGGT
GTTCCAGCTCCATCGCATGGTATCATTGAGGAAATTTTTGTTTCAGACGGTGATACAGTA
AAGGCTGGACAACAGTTGTTCAAAATGAAGTTTACAGATGCCGCACCAGAAAAAGAAGCA
TCAACTTCCACGCCAGAACCTCCAAAAGCAGCTGCTGCACCTTCACCACCACCACCACAA
CCTAAACAAGAAGCACCAAAAGCTGCTCCTCAACCACAAGCAGCACCACCACCTGCTGCA
TCAACACCTCCACCACCGCCTCCAAAACCAGCAGCACCAGCAAAAACCGTTCCAATTGCT
GCCATTCGACATGCACAAGCTATCGATGCTGCCACAGTTAAAGTACCTCCAGCTGATTAT
ACAAAAGAAATCACTGGCACACGCACGGAACAGCGTGTAAAAATGAATCGAATGCGTCTA
AAGATTGCATCACGTTTGAAGGAAGCACAAAATGTCAATGCAATGTTAACAACATTCAAT
GAAATTGATATGAGTGCAATCATGGAATTCCGCAAGTTGCATCAAGAAACCTTCCAAAAG
CGTTATGGAATTAAATTAGGATTTATGTCAGCTTTCTGTAAAGCTGCTGCTTATGCTCTT
CAAGATCAACCAGTTGTTAATGCTGTTATTGAAGATAA

>g635.t7 Gene=g635 Length=332
MIRSLSSFPRSVVLRISNRSNQIQQTLRNNIHQQCAVQKLVLTCDTNSQNSTLKNRLVMQ
QQHVRLIQTTTQLLNSEIVKVPPFADSVSEGDVKFDKKVGDAVAADEVVMEIETDKTTVG
VPAPSHGIIEEIFVSDGDTVKAGQQLFKMKFTDAAPEKEASTSTPEPPKAAAAPSPPPPQ
PKQEAPKAAPQPQAAPPPAASTPPPPPPKPAAPAKTVPIAAIRHAQAIDAATVKVPPADY
TKEITGTRTEQRVKMNRMRLKIASRLKEAQNVNAMLTTFNEIDMSAIMEFRKLHQETFQK
RYGIKLGFMSAFCKAAAYALQDQPVVNAVIED

Protein features from InterProScan

Transcript Database ID Name Start End E.value
9 g635.t7 CDD cd06849 lipoyl_domain 78 149 2.3549E-22
7 g635.t7 Gene3D G3DSA:2.40.50.100 - 74 175 4.5E-18
8 g635.t7 Gene3D G3DSA:3.30.559.10 Chloramphenicol Acetyltransferase 247 332 2.0E-20
12 g635.t7 MobiDBLite mobidb-lite consensus disorder prediction 153 214 -
11 g635.t7 MobiDBLite mobidb-lite consensus disorder prediction 169 214 -
3 g635.t7 PANTHER PTHR43416 DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED 63 332 5.6E-65
4 g635.t7 PANTHER PTHR43416:SF18 DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL 63 332 5.6E-65
1 g635.t7 Pfam PF00364 Biotin-requiring enzyme 79 149 7.3E-18
2 g635.t7 Pfam PF00198 2-oxoacid dehydrogenases acyltransferase (catalytic domain) 249 331 8.9E-15
10 g635.t7 ProSitePatterns PS00189 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site. 100 129 -
13 g635.t7 ProSiteProfiles PS50968 Biotinyl/lipoyl domain profile. 76 150 24.11
5 g635.t7 SUPERFAMILY SSF51230 Single hybrid motif 77 168 9.29E-20
6 g635.t7 SUPERFAMILY SSF52777 CoA-dependent acyltransferases 238 331 2.36E-19

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0016746 acyltransferase activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values