General information for g635.t8
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial.
Parent gene
- The parent gene g635 is located on chr_3: 4682651..4683238 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g635 | g635.t8 | TSS | g635.t8 | 4679520 | 4679520 |
| chr_3 | g635 | g635.t8 | isoform | g635.t8 | 4679597 | 4682070 |
| chr_3 | g635 | g635.t8 | exon | g635.t8.exon1 | 4679597 | 4679662 |
| chr_3 | g635 | g635.t8 | cds | g635.t8.CDS1 | 4679597 | 4679662 |
| chr_3 | g635 | g635.t8 | exon | g635.t8.exon2 | 4679989 | 4680068 |
| chr_3 | g635 | g635.t8 | cds | g635.t8.CDS2 | 4679989 | 4680068 |
| chr_3 | g635 | g635.t8 | exon | g635.t8.exon3 | 4680127 | 4680255 |
| chr_3 | g635 | g635.t8 | cds | g635.t8.CDS3 | 4680127 | 4680255 |
| chr_3 | g635 | g635.t8 | exon | g635.t8.exon4 | 4680321 | 4680606 |
| chr_3 | g635 | g635.t8 | cds | g635.t8.CDS4 | 4680321 | 4680606 |
| chr_3 | g635 | g635.t8 | exon | g635.t8.exon5 | 4681640 | 4682070 |
| chr_3 | g635 | g635.t8 | cds | g635.t8.CDS5 | 4681640 | 4682068 |
| chr_3 | g635 | g635.t8 | TTS | g635.t8 | 4682871 | 4682871 |
Sequences
>g635.t8 Gene=g635 Length=992
ATGATTCGATCACTTTCCTCATTTCCACGAAGTGTTGTTCTTCGCATAAGCAATAGAAGT
AATCAGCAAACGTTGAGGAATAATATTCATCAACAATGTGCCGTTCAAAAGTTGGTGCTT
ACATGCGACACAAATTCTCAAAATTCAACACTTAAAAATAGATTAGTTATGCAGCAACAA
CATGTTAGATTAATTCAAACAACTACGCAACTTCTTAATTCTGAAATCGTTAAGGTGCCA
CCATTTGCCGATTCTGTATCAGAAGGTGATGTAAAATTTGATAAGAAAGTAGGTGATGCA
GTTGCAGCTGATGAGGTTGTTATGGAAATTGAAACCGATAAGACAACAGTTGGTGTTCCA
GCTCCATCGCATGGTATCATTGAGGAAATTTTTGTTTCAGACGGTGATACAGTAAAGGCT
GGACAACAGTTGTTCAAAATGAAGTTTACAGATGCCGCACCAGAAAAAGAAGCATCAACT
TCCACGCCAGAACCTCCAAAAGCAGCTGCTGCACCTTCACCACCACCACCACAACCTAAA
CAAGAAGCACCAAAAGCTGCTCCTCAACCACAAGCAGCACCACCACCTGCTGCATCAACA
CCTCCACCACCGCCTCCAAAACCAGCAGCACCAGCAAAAACCGTTCCAATTGCTGCCATT
CGACATGCACAAGCTATCGATGCTGCCACAGTTAAAGTACCTCCAGCTGATTATACAAAA
GAAATCACTGGCACACGCACGGAACAGCGTGTAAAAATGAATCGAATGCGTCTAAAGATT
GCATCACGTTTGAAGGAAGCACAAAATGTCAATGCAATGTTAACAACATTCAATGAAATT
GATATGAGTGCAATCATGGAATTCCGCAAGTTGCATCAAGAAACCTTCCAAAAGCGTTAT
GGAATTAAATTAGGATTTATGTCAGCTTTCTGTAAAGCTGCTGCTTATGCTCTTCAAGAT
CAACCAGTTGTTAATGCTGTTATTGAAGATAA
>g635.t8 Gene=g635 Length=330
MIRSLSSFPRSVVLRISNRSNQQTLRNNIHQQCAVQKLVLTCDTNSQNSTLKNRLVMQQQ
HVRLIQTTTQLLNSEIVKVPPFADSVSEGDVKFDKKVGDAVAADEVVMEIETDKTTVGVP
APSHGIIEEIFVSDGDTVKAGQQLFKMKFTDAAPEKEASTSTPEPPKAAAAPSPPPPQPK
QEAPKAAPQPQAAPPPAASTPPPPPPKPAAPAKTVPIAAIRHAQAIDAATVKVPPADYTK
EITGTRTEQRVKMNRMRLKIASRLKEAQNVNAMLTTFNEIDMSAIMEFRKLHQETFQKRY
GIKLGFMSAFCKAAAYALQDQPVVNAVIED
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g635.t8 | CDD | cd06849 | lipoyl_domain | 76 | 147 | 2.13038E-22 |
| 7 | g635.t8 | Gene3D | G3DSA:2.40.50.100 | - | 72 | 173 | 4.4E-18 |
| 8 | g635.t8 | Gene3D | G3DSA:3.30.559.10 | Chloramphenicol Acetyltransferase | 245 | 330 | 2.0E-20 |
| 11 | g635.t8 | MobiDBLite | mobidb-lite | consensus disorder prediction | 151 | 212 | - |
| 12 | g635.t8 | MobiDBLite | mobidb-lite | consensus disorder prediction | 167 | 212 | - |
| 3 | g635.t8 | PANTHER | PTHR43416 | DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED | 60 | 330 | 4.4E-65 |
| 4 | g635.t8 | PANTHER | PTHR43416:SF18 | DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL | 60 | 330 | 4.4E-65 |
| 1 | g635.t8 | Pfam | PF00364 | Biotin-requiring enzyme | 77 | 147 | 7.2E-18 |
| 2 | g635.t8 | Pfam | PF00198 | 2-oxoacid dehydrogenases acyltransferase (catalytic domain) | 247 | 329 | 8.8E-15 |
| 10 | g635.t8 | ProSitePatterns | PS00189 | 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site. | 98 | 127 | - |
| 13 | g635.t8 | ProSiteProfiles | PS50968 | Biotinyl/lipoyl domain profile. | 74 | 148 | 24.11 |
| 5 | g635.t8 | SUPERFAMILY | SSF51230 | Single hybrid motif | 75 | 166 | 9.16E-20 |
| 6 | g635.t8 | SUPERFAMILY | SSF52777 | CoA-dependent acyltransferases | 247 | 329 | 2.31E-19 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016746 | acyltransferase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
