General information for g6513.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Insulin gene enhancer protein ISL-1.
Parent gene
- The parent gene g6513 is located on chr_2: 17396545..17397563 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6513 | g6513.t2 | TSS | g6513.t2 | 17395590 | 17395590 |
| chr_2 | g6513 | g6513.t2 | isoform | g6513.t2 | 17395768 | 17397563 |
| chr_2 | g6513 | g6513.t2 | exon | g6513.t2.exon1 | 17395768 | 17395909 |
| chr_2 | g6513 | g6513.t2 | exon | g6513.t2.exon2 | 17396009 | 17396333 |
| chr_2 | g6513 | g6513.t2 | cds | g6513.t2.CDS1 | 17396261 | 17396333 |
| chr_2 | g6513 | g6513.t2 | exon | g6513.t2.exon3 | 17396545 | 17396751 |
| chr_2 | g6513 | g6513.t2 | cds | g6513.t2.CDS2 | 17396545 | 17396751 |
| chr_2 | g6513 | g6513.t2 | exon | g6513.t2.exon4 | 17396814 | 17397103 |
| chr_2 | g6513 | g6513.t2 | cds | g6513.t2.CDS3 | 17396814 | 17397103 |
| chr_2 | g6513 | g6513.t2 | exon | g6513.t2.exon5 | 17397175 | 17397369 |
| chr_2 | g6513 | g6513.t2 | cds | g6513.t2.CDS4 | 17397175 | 17397369 |
| chr_2 | g6513 | g6513.t2 | exon | g6513.t2.exon6 | 17397432 | 17397563 |
| chr_2 | g6513 | g6513.t2 | cds | g6513.t2.CDS5 | 17397432 | 17397563 |
| chr_2 | g6513 | g6513.t2 | TTS | g6513.t2 | 17397641 | 17397641 |
Sequences
>g6513.t2 Gene=g6513 Length=1291
ATGGCAGACATTAAAGGTGGCTTACTACAGCCACAATCATTTAATCATCATCGGCCATAT
CATCATCACGATGAATTTATACAACCAACTTTAATCGAAAACGAAAATTGTGGAAATTAT
GCATACAATCAGAGTGGAATAAAAATGGAAGCAAAAAAGTCATTTTTGTGTTGGATGTTC
GCTAGAAATTCATGATCAATATATTTTGCGAGTAGCACCTGATTTGGAATGGCATGCTGC
ATGCCTCAAATGTCAAGAGTGTCGTCAGTTCTTGGATGAAAGCTGTACGTGTTTTGTACG
CGATGGCAAGACATATTGCAAACGCGATTACGTTCGACTATTTGGTACCAAGTGTGATAA
GTGCGGAAATTCATTCTGTAAAAATGACTTTGTGATGCGAGCTAAAACTAAAATTTATCA
TATTAAATGTTTTCGATGCTCAGCTTGTGAAAGACAATTGAATCCAGGTGATGAATTTGC
ATTGCGTGATGGTGGTGCACTATATTGTAAAAATGATCACGATCAAATAGAGAAAATGAA
GTCTGAAGAAAGATCTTCTTGTCAGTTAGCTGAGTTGAATAATAATAAACCTACACAAGA
ATCTACAATTAAGCAACAATCAACGTGCAATATTAAACGTTCATCTTCAAGTGATGATTT
TGGTTCATTGTCCGATTCTGACAGTGAATCCGACTCGTATAAATCTTCATCAAATCGACG
TGAAAAGCATACGCATACATTAAATGGCAAGCCAGCTCGAGTGAGAACAGTGTTAAATGA
GAAGCAACTAAGTATGCTAAGAGCATGTTATCAGATGAATTCAAGACCAGACTCATTGGT
TAAAGAGCAGCTAGTCGAAATGACGGGACTGTCTGCTCGAGTCGTCCGTGTTTGGTTCCA
AAATAAACGATGCAAAGATAAGAAAAAGGCCATTGAGGTAAAAATGCAGATGCATCAAGA
AAAGGAAGGAAAAATTTCATTCATGCAAGGCATTCCATTGATTGCATCGCCACCAGTGAA
ACATGAGTCGCCAGTCAATCATCAAGGTTATGAAGTCACTAAATATCAACCACCATGGAG
AACTTTCTCAGACTTTGCCATGCAAAATGACTCTGATGATTGCACGTCAACTCCCGCATT
TCAACATTTGGTCAATCAGCTTCATGGTTACGATTTAAGTGAAGAGGCGGCACTACACAA
TTACAATGATCACTCGATGGACTGGCATAAGAATCGTGAAAGTACTGACTCATATCAGGA
AAGCGAGGGAAGCATTTCCGGCTCACTATAA
>g6513.t2 Gene=g6513 Length=298
MRAKTKIYHIKCFRCSACERQLNPGDEFALRDGGALYCKNDHDQIEKMKSEERSSCQLAE
LNNNKPTQESTIKQQSTCNIKRSSSSDDFGSLSDSDSESDSYKSSSNRREKHTHTLNGKP
ARVRTVLNEKQLSMLRACYQMNSRPDSLVKEQLVEMTGLSARVVRVWFQNKRCKDKKKAI
EVKMQMHQEKEGKISFMQGIPLIASPPVKHESPVNHQGYEVTKYQPPWRTFSDFAMQNDS
DDCTSTPAFQHLVNQLHGYDLSEEAALHNYNDHSMDWHKNRESTDSYQESEGSISGSL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g6513.t2 | CDD | cd00086 | homeodomain | 122 | 178 | 2.75876E-14 |
| 8 | g6513.t2 | Coils | Coil | Coil | 170 | 190 | - |
| 7 | g6513.t2 | Gene3D | G3DSA:2.10.110.10 | Cysteine Rich Protein | 1 | 96 | 3.6E-13 |
| 6 | g6513.t2 | Gene3D | G3DSA:1.10.10.60 | - | 119 | 182 | 5.9E-19 |
| 13 | g6513.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 61 | 122 | - |
| 15 | g6513.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 61 | 100 | - |
| 14 | g6513.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 101 | 117 | - |
| 3 | g6513.t2 | PANTHER | PTHR24204 | INSULIN GENE ENHANCER PROTEIN | 1 | 263 | 1.4E-82 |
| 4 | g6513.t2 | PANTHER | PTHR24204:SF8 | TAILUP, ISOFORM A | 1 | 263 | 1.4E-82 |
| 2 | g6513.t2 | Pfam | PF00412 | LIM domain | 1 | 43 | 7.5E-12 |
| 1 | g6513.t2 | Pfam | PF00046 | Homeodomain | 122 | 177 | 5.9E-15 |
| 12 | g6513.t2 | ProSitePatterns | PS00027 | ‘Homeobox’ domain signature. | 153 | 176 | - |
| 17 | g6513.t2 | ProSiteProfiles | PS50023 | LIM domain profile. | 1 | 48 | 10.138 |
| 16 | g6513.t2 | ProSiteProfiles | PS50071 | ‘Homeobox’ domain profile. | 118 | 178 | 15.823 |
| 10 | g6513.t2 | SMART | SM00132 | lim_4 | 1 | 41 | 0.0041 |
| 11 | g6513.t2 | SMART | SM00389 | HOX_1 | 120 | 182 | 3.5E-18 |
| 5 | g6513.t2 | SUPERFAMILY | SSF46689 | Homeodomain-like | 104 | 178 | 1.67E-17 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003677 | DNA binding | MF |
| GO:0000981 | DNA-binding transcription factor activity, RNA polymerase II-specific | MF |
| GO:0006355 | regulation of transcription, DNA-templated | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed