General information for g6519.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Tyrosine-protein kinase Btk29A.
Parent gene
- The parent gene g6519 is located on chr_2: 17454062..17455300 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6519 | g6519.t1 | TSS | g6519.t1 | 17447857 | 17447857 |
| chr_2 | g6519 | g6519.t1 | isoform | g6519.t1 | 17448462 | 17454732 |
| chr_2 | g6519 | g6519.t1 | exon | g6519.t1.exon1 | 17448462 | 17448614 |
| chr_2 | g6519 | g6519.t1 | cds | g6519.t1.CDS1 | 17448462 | 17448614 |
| chr_2 | g6519 | g6519.t1 | exon | g6519.t1.exon2 | 17449291 | 17449386 |
| chr_2 | g6519 | g6519.t1 | cds | g6519.t1.CDS2 | 17449291 | 17449386 |
| chr_2 | g6519 | g6519.t1 | exon | g6519.t1.exon3 | 17449451 | 17449580 |
| chr_2 | g6519 | g6519.t1 | cds | g6519.t1.CDS3 | 17449451 | 17449580 |
| chr_2 | g6519 | g6519.t1 | exon | g6519.t1.exon4 | 17449649 | 17449795 |
| chr_2 | g6519 | g6519.t1 | cds | g6519.t1.CDS4 | 17449649 | 17449795 |
| chr_2 | g6519 | g6519.t1 | exon | g6519.t1.exon5 | 17451905 | 17452107 |
| chr_2 | g6519 | g6519.t1 | cds | g6519.t1.CDS5 | 17451905 | 17452107 |
| chr_2 | g6519 | g6519.t1 | exon | g6519.t1.exon6 | 17452726 | 17452872 |
| chr_2 | g6519 | g6519.t1 | cds | g6519.t1.CDS6 | 17452726 | 17452872 |
| chr_2 | g6519 | g6519.t1 | exon | g6519.t1.exon7 | 17452943 | 17453023 |
| chr_2 | g6519 | g6519.t1 | cds | g6519.t1.CDS7 | 17452943 | 17453023 |
| chr_2 | g6519 | g6519.t1 | exon | g6519.t1.exon8 | 17453080 | 17453210 |
| chr_2 | g6519 | g6519.t1 | cds | g6519.t1.CDS8 | 17453080 | 17453210 |
| chr_2 | g6519 | g6519.t1 | exon | g6519.t1.exon9 | 17453272 | 17453594 |
| chr_2 | g6519 | g6519.t1 | cds | g6519.t1.CDS9 | 17453272 | 17453594 |
| chr_2 | g6519 | g6519.t1 | exon | g6519.t1.exon10 | 17453659 | 17453999 |
| chr_2 | g6519 | g6519.t1 | cds | g6519.t1.CDS10 | 17453659 | 17453999 |
| chr_2 | g6519 | g6519.t1 | exon | g6519.t1.exon11 | 17454062 | 17454235 |
| chr_2 | g6519 | g6519.t1 | cds | g6519.t1.CDS11 | 17454062 | 17454235 |
| chr_2 | g6519 | g6519.t1 | exon | g6519.t1.exon12 | 17454295 | 17454358 |
| chr_2 | g6519 | g6519.t1 | cds | g6519.t1.CDS12 | 17454295 | 17454358 |
| chr_2 | g6519 | g6519.t1 | exon | g6519.t1.exon13 | 17454424 | 17454557 |
| chr_2 | g6519 | g6519.t1 | cds | g6519.t1.CDS13 | 17454424 | 17454557 |
| chr_2 | g6519 | g6519.t1 | exon | g6519.t1.exon14 | 17454634 | 17454732 |
| chr_2 | g6519 | g6519.t1 | cds | g6519.t1.CDS14 | 17454634 | 17454732 |
| chr_2 | g6519 | g6519.t1 | TTS | g6519.t1 | 17455296 | 17455296 |
Sequences
>g6519.t1 Gene=g6519 Length=2223
ATGAGTGAGACAACAATGTTGGTTGAACCAACTTTGATGATAAAACGTAGTCAAAACAAA
AAGAGATGGTCTCTAATAAATTATAAGACAAGATTGTTTGAATTGTCGCGAACACATTTG
GTTTATTTTGACAATATCGATGGGGGCAAGGAGAAACGTAGAGAAAGAGGACGCATTGAC
GTGCGCGGCATCAAACTAGTGGAACCTGCAATTTTACACGGTGATGGAGGCGATGTTCAT
GCTCCAAATGGATATCCTTTTCAAATAGGCTATTCAGAGAACGGCATAAATAGTAATAGC
ACAGTACCAACTTTCACCCTATATCTCGTTGCTGCCACAGATAAAGAAAGAAGTGAATGG
ATTAGAGCAATAAGAAAAGTCAGTGAAGATTATTCACCAAAATCATTTCGATATCATCCG
GGATTGTTTAAAATCAATAAATGGTCTTGCTGTAAAAACACAAGTCGAACTGCACTTGGC
TGTCTGGTGACAACTAGTTGGCCGGAAAAAAATAATAATTATAAAAATAACGGAAATAAT
AACTCACCAAGCCAGAATTCAACTCGAAGTACTAGTCCTAATAGAACCAATAACAATAAT
AGCACTAGCAGCGGTCATATACAAAAAGTCTCATCTCAAACACAAATACAACTACAAGGT
TCTCCGCAACAGCAACTGCAGAGTCACACATTATTAAATAACTCAACTCCATTCAACTCT
CCTCATCAGACATCGTTAAATAACTTTAAATCGCCATCACTTAACGGAAGTGGTAACTGT
CAGCACGTTGACATGCCTATGCCAGGAAGTACAGGAACTACAACCACAACAACTTCAGCA
ATTCCACCGCCTGGAACACCAAAGGTAAAAGCAAAAGATGGAACAGGACGATTAAAAATA
GTTGTGGCACTATATCCATTTAAAGCAATAGAGACTGGAGATTTATCATTAGAAAAGAAT
GCTGAATATGAAGTTATTGATGATTCACAAGAGCATTGGTGGAAAGTTAAAGATTCATCT
GGAAATATCGGTTACATTCCATCAAATTATGTAAAAGAAAAAGAACTTTTAGGCCTACAG
AAATATGATTGGTATGTCGGCGACATGTCGAGACAGCGTGCAGAGTCATTATTAAAGCAA
AATGACAAAGAAGGCTGCTTCGTGGTTCGCAAGTCATCAACAAAAGGTCTATACACACTC
TCGTTACACACCAAAGTTCCGCAATCGCATGTCAAGCACTATCATATTAAGCAAAATGCA
CGAGGAGAATTTTATTTAAGCGAAAAACACTGCTGTGAAACTATTCCCGATCTTATCAAC
TATCATCGACACAATTCCGGTGGATTAGCGTGTCGATTAAAAGTTTCGCCATGTGATCGT
CCTGTTCCGCCCACTGCCGGTCTTAGTCATGATAAATGGGAAATTCATCCATCTGAACTA
ATGATACTTGAAGAACTCGGTTCGGGCCAATTTGGTGTTGTTCGTCGTGCTAAATGGCGC
GGATCAATTGATACTGCTGTTAAAATGATGAAAGTCGGAACAATGTCGGAAGATGATTTT
ATTGAAGAAGCGAAAGTCATGACTAAATTGCAACATCCTAATCTTGTGCAGCTTTATGGT
GTATGCAGCAAACATCGTCCAATTTATATCATTACTGAATATATGAAGCATGGTTCGCTA
TTAAATTATTTAAGAAGACACGAACAGTCATTAATTGGCAATATGGGATTACTTTTGGAT
ATGTGCATACAAGTTTGCAAAGGAATGTCCTATTTAGAGCGTCATAATTATATTCATCGC
GATTTAGCAGCTCGTAATTGTTTAGTCGGCAGCGAAAATACAGTAAAAGTGGCAGACTTT
GGTTTAGCTCGTTATGTGCTTGATGACCAATATACATCTTCAGGCGGTACAAAGTTTCCC
ATAAAGTGGGCACCTCCAGAGGTCTTAAACTACACAAGATTCTCCTCGAAGTCTGACGTT
TGGGCATATGGTGTTTTAATGTGGGAAGTTTTTACTTGTGGCAAAATGCCATACGGTCGC
CTAAAAAATACCGAAGTTGTTGAAAGAGTTCAGCGTGGAATAATTTTAGAGAAACCAAAA
GCATGTGCAAAAGAAATTTATGATGTAATGAGAAAGTGCTGGTCTCATGGCCCGGAAGAT
CGACCGAGCTTTCGTTTACTAAAAGATCAACTTGCGACTGTAGCACAAGGATTAACCGAC
TAA
>g6519.t1 Gene=g6519 Length=740
MSETTMLVEPTLMIKRSQNKKRWSLINYKTRLFELSRTHLVYFDNIDGGKEKRRERGRID
VRGIKLVEPAILHGDGGDVHAPNGYPFQIGYSENGINSNSTVPTFTLYLVAATDKERSEW
IRAIRKVSEDYSPKSFRYHPGLFKINKWSCCKNTSRTALGCLVTTSWPEKNNNYKNNGNN
NSPSQNSTRSTSPNRTNNNNSTSSGHIQKVSSQTQIQLQGSPQQQLQSHTLLNNSTPFNS
PHQTSLNNFKSPSLNGSGNCQHVDMPMPGSTGTTTTTTSAIPPPGTPKVKAKDGTGRLKI
VVALYPFKAIETGDLSLEKNAEYEVIDDSQEHWWKVKDSSGNIGYIPSNYVKEKELLGLQ
KYDWYVGDMSRQRAESLLKQNDKEGCFVVRKSSTKGLYTLSLHTKVPQSHVKHYHIKQNA
RGEFYLSEKHCCETIPDLINYHRHNSGGLACRLKVSPCDRPVPPTAGLSHDKWEIHPSEL
MILEELGSGQFGVVRRAKWRGSIDTAVKMMKVGTMSEDDFIEEAKVMTKLQHPNLVQLYG
VCSKHRPIYIITEYMKHGSLLNYLRRHEQSLIGNMGLLLDMCIQVCKGMSYLERHNYIHR
DLAARNCLVGSENTVKVADFGLARYVLDDQYTSSGGTKFPIKWAPPEVLNYTRFSSKSDV
WAYGVLMWEVFTCGKMPYGRLKNTEVVERVQRGIILEKPKACAKEIYDVMRKCWSHGPED
RPSFRLLKDQLATVAQGLTD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 26 | g6519.t1 | CDD | cd11768 | SH3_Tec_like | 300 | 353 | 1.86638E-29 |
| 28 | g6519.t1 | CDD | cd09934 | SH2_Tec_family | 357 | 459 | 1.48395E-66 |
| 27 | g6519.t1 | CDD | cd05059 | PTKc_Tec_like | 475 | 732 | 8.74696E-177 |
| 23 | g6519.t1 | Gene3D | G3DSA:2.30.29.30 | - | 2 | 170 | 1.8E-32 |
| 22 | g6519.t1 | Gene3D | G3DSA:2.30.30.40 | SH3 Domains | 293 | 355 | 3.3E-19 |
| 25 | g6519.t1 | Gene3D | G3DSA:3.30.505.10 | SHC Adaptor Protein | 356 | 466 | 3.9E-32 |
| 24 | g6519.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 473 | 737 | 4.9E-95 |
| 35 | g6519.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 172 | 227 | - |
| 36 | g6519.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 271 | 292 | - |
| 6 | g6519.t1 | PANTHER | PTHR24418 | TYROSINE-PROTEIN KINASE | 280 | 735 | 3.2E-200 |
| 7 | g6519.t1 | PANTHER | PTHR24418:SF219 | TYROSINE-PROTEIN KINASE TEC | 280 | 735 | 3.2E-200 |
| 10 | g6519.t1 | PRINTS | PR00401 | SH2 domain signature | 364 | 378 | 2.2E-8 |
| 12 | g6519.t1 | PRINTS | PR00401 | SH2 domain signature | 384 | 394 | 2.2E-8 |
| 11 | g6519.t1 | PRINTS | PR00401 | SH2 domain signature | 395 | 406 | 2.2E-8 |
| 9 | g6519.t1 | PRINTS | PR00401 | SH2 domain signature | 410 | 420 | 2.2E-8 |
| 8 | g6519.t1 | PRINTS | PR00401 | SH2 domain signature | 431 | 445 | 2.2E-8 |
| 15 | g6519.t1 | PRINTS | PR00109 | Tyrosine kinase catalytic domain signature | 552 | 565 | 1.5E-35 |
| 17 | g6519.t1 | PRINTS | PR00109 | Tyrosine kinase catalytic domain signature | 591 | 609 | 1.5E-35 |
| 14 | g6519.t1 | PRINTS | PR00109 | Tyrosine kinase catalytic domain signature | 639 | 649 | 1.5E-35 |
| 13 | g6519.t1 | PRINTS | PR00109 | Tyrosine kinase catalytic domain signature | 658 | 680 | 1.5E-35 |
| 16 | g6519.t1 | PRINTS | PR00109 | Tyrosine kinase catalytic domain signature | 702 | 724 | 1.5E-35 |
| 3 | g6519.t1 | Pfam | PF00169 | PH domain | 13 | 128 | 2.3E-11 |
| 4 | g6519.t1 | Pfam | PF00779 | BTK motif | 137 | 166 | 5.5E-11 |
| 1 | g6519.t1 | Pfam | PF00018 | SH3 domain | 302 | 348 | 1.2E-13 |
| 2 | g6519.t1 | Pfam | PF00017 | SH2 domain | 364 | 442 | 5.9E-22 |
| 5 | g6519.t1 | Pfam | PF07714 | Protein tyrosine and serine/threonine kinase | 481 | 731 | 4.1E-96 |
| 34 | g6519.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 486 | 508 | - |
| 33 | g6519.t1 | ProSitePatterns | PS00109 | Tyrosine protein kinases specific active-site signature. | 597 | 609 | - |
| 41 | g6519.t1 | ProSiteProfiles | PS50003 | PH domain profile. | 6 | 129 | 12.098 |
| 38 | g6519.t1 | ProSiteProfiles | PS51113 | Zinc finger Btk-type profile. | 131 | 167 | 13.383 |
| 37 | g6519.t1 | ProSiteProfiles | PS50002 | Src homology 3 (SH3) domain profile. | 296 | 356 | 25.566 |
| 39 | g6519.t1 | ProSiteProfiles | PS50001 | Src homology 2 (SH2) domain profile. | 364 | 458 | 20.622 |
| 40 | g6519.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 480 | 734 | 40.852 |
| 31 | g6519.t1 | SMART | SM00233 | PH_update | 12 | 131 | 3.8E-11 |
| 30 | g6519.t1 | SMART | SM00326 | SH3_2 | 299 | 355 | 4.4E-18 |
| 32 | g6519.t1 | SMART | SM00252 | SH2_5 | 362 | 448 | 1.6E-30 |
| 29 | g6519.t1 | SMART | SM00219 | tyrkin_6 | 480 | 731 | 2.3E-137 |
| 18 | g6519.t1 | SUPERFAMILY | SSF50729 | PH domain-like | 13 | 164 | 7.0E-26 |
| 21 | g6519.t1 | SUPERFAMILY | SSF50044 | SH3-domain | 276 | 354 | 4.62E-16 |
| 20 | g6519.t1 | SUPERFAMILY | SSF55550 | SH2 domain | 332 | 455 | 4.98E-32 |
| 19 | g6519.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 470 | 731 | 6.97E-79 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004713 | protein tyrosine kinase activity | MF |
| GO:0005524 | ATP binding | MF |
| GO:0005515 | protein binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
| GO:0035556 | intracellular signal transduction | BP |
KEGG
Orthology
- This transcript has been annotated with K07364 (tyrosine-protein kinase Tec)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
