Gene loci information

Transcript annotation

  • This transcript has been annotated as Tyrosine-protein kinase Btk29A.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g6519 g6519.t5 isoform g6519.t5 17454062 17455300
chr_2 g6519 g6519.t5 exon g6519.t5.exon1 17454062 17454235
chr_2 g6519 g6519.t5 cds g6519.t5.CDS1 17454074 17454235
chr_2 g6519 g6519.t5 exon g6519.t5.exon2 17454295 17454358
chr_2 g6519 g6519.t5 cds g6519.t5.CDS2 17454295 17454358
chr_2 g6519 g6519.t5 exon g6519.t5.exon3 17454424 17454557
chr_2 g6519 g6519.t5 cds g6519.t5.CDS3 17454424 17454557
chr_2 g6519 g6519.t5 exon g6519.t5.exon4 17454634 17455300
chr_2 g6519 g6519.t5 cds g6519.t5.CDS4 17454634 17454732
chr_2 g6519 g6519.t5 TTS g6519.t5 17455296 17455296
chr_2 g6519 g6519.t5 TSS g6519.t5 NA NA

Sequences

>g6519.t5 Gene=g6519 Length=1039
GTTTGCAAAGGAATGTCCTATTTAGAGCGTCATAATTATATTCATCGCGATTTAGCAGCT
CGTAATTGTTTAGTCGGCAGCGAAAATACAGTAAAAGTGGCAGACTTTGGTTTAGCTCGT
TATGTGCTTGATGACCAATATACATCTTCAGGCGGTACAAAGTTTCCCATAAAGTGGGCA
CCTCCAGAGGTCTTAAACTACACAAGATTCTCCTCGAAGTCTGACGTTTGGGCATATGGT
GTTTTAATGTGGGAAGTTTTTACTTGTGGCAAAATGCCATACGGTCGCCTAAAAAATACC
GAAGTTGTTGAAAGAGTTCAGCGTGGAATAATTTTAGAGAAACCAAAAGCATGTGCAAAA
GAAATTTATGATGTAATGAGAAAGTGCTGGTCTCATGGCCCGGAAGATCGACCGAGCTTT
CGTTTACTAAAAGATCAACTTGCGACTGTAGCACAAGGATTAACCGACTAAAAATGTGGA
GATTTTTATACAATCGTGTTTTTTTCTTTTCCTCTTTTGACTTTTCATGTTTTTTATACG
AACTTAACAACAAAGTTTTTTTAGTTTTATGCTTGACAATGATTTTGATTTGATTCTGAG
TGTTTTGATGTCATCAAATTATTAGTTAGATAATATCACACAAACACATACATTACCTAT
AATTATTAAACAATTTTTTTCTTAAATAAGAAATTTAACTCACAAAAACACAAAAATATC
TAGTTTATAATAATTATAATAAAAATTATAATGATATTTCTTCCTTCAGACAAACACTGT
CTTCGATAGCTAAAAATAATCATTTCTCGCTGATAATAACAATACAATAAGATAAAAAAG
ACATGAAAATACTCAAAGGAACTAAACTAAACTCATTTTGCGTAATATCATCCTATATAT
TGCCACACAAATTTTTATGGAGAATACTAGTAGTATGAAAAATAATATCATTAAAGATAA
AATTAGAAAAGAAGAATATAAGAAAAAACTTTGCTATTTTGTACTCCTTGATTCAATAGT
TAAGTTGAGTGAAGCAAAA

>g6519.t5 Gene=g6519 Length=152
MSYLERHNYIHRDLAARNCLVGSENTVKVADFGLARYVLDDQYTSSGGTKFPIKWAPPEV
LNYTRFSSKSDVWAYGVLMWEVFTCGKMPYGRLKNTEVVERVQRGIILEKPKACAKEIYD
VMRKCWSHGPEDRPSFRLLKDQLATVAQGLTD

Protein features from InterProScan

Transcript Database ID Name Start End E.value
9 g6519.t5 Gene3D G3DSA:1.10.510.10 Transferase(Phosphotransferase) domain 1 1 149 2.2E-55
2 g6519.t5 PANTHER PTHR24418 TYROSINE-PROTEIN KINASE 1 148 1.7E-71
3 g6519.t5 PANTHER PTHR24418:SF61 TYROSINE-PROTEIN KINASE ITK/TSK 1 148 1.7E-71
7 g6519.t5 PRINTS PR00109 Tyrosine kinase catalytic domain signature 3 21 7.2E-31
6 g6519.t5 PRINTS PR00109 Tyrosine kinase catalytic domain signature 51 61 7.2E-31
5 g6519.t5 PRINTS PR00109 Tyrosine kinase catalytic domain signature 70 92 7.2E-31
4 g6519.t5 PRINTS PR00109 Tyrosine kinase catalytic domain signature 114 136 7.2E-31
1 g6519.t5 Pfam PF07714 Protein tyrosine and serine/threonine kinase 1 143 4.9E-59
10 g6519.t5 ProSitePatterns PS00109 Tyrosine protein kinases specific active-site signature. 9 21 -
12 g6519.t5 ProSiteProfiles PS50011 Protein kinase domain profile. 1 146 25.46
11 g6519.t5 SMART SM00219 tyrkin_6 1 143 1.3E-33
8 g6519.t5 SUPERFAMILY SSF56112 Protein kinase-like (PK-like) 1 143 2.47E-45

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004713 protein tyrosine kinase activity MF
GO:0005524 ATP binding MF
GO:0004672 protein kinase activity MF
GO:0006468 protein phosphorylation BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values