General information for g6585.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase.
Parent gene
- The parent gene g6585 is located on chr_2: 17717853..17718946 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6585 | g6585.t1 | TTS | g6585.t1 | 17716314 | 17716314 |
| chr_2 | g6585 | g6585.t1 | isoform | g6585.t1 | 17716396 | 17717853 |
| chr_2 | g6585 | g6585.t1 | exon | g6585.t1.exon1 | 17716396 | 17716988 |
| chr_2 | g6585 | g6585.t1 | cds | g6585.t1.CDS1 | 17716396 | 17716988 |
| chr_2 | g6585 | g6585.t1 | exon | g6585.t1.exon2 | 17717050 | 17717641 |
| chr_2 | g6585 | g6585.t1 | cds | g6585.t1.CDS2 | 17717050 | 17717641 |
| chr_2 | g6585 | g6585.t1 | exon | g6585.t1.exon3 | 17717710 | 17717853 |
| chr_2 | g6585 | g6585.t1 | cds | g6585.t1.CDS3 | 17717710 | 17717853 |
| chr_2 | g6585 | g6585.t1 | TSS | g6585.t1 | 17717854 | 17717854 |
Sequences
>g6585.t1 Gene=g6585 Length=1329
ATGCATTCAAGTATTTTAATTGGATTTTATTCGATAATAACATTGATTTTGTTTGATAAA
GTTTATCAAACATCACAATCAATAATAGACGAATTATTTCATATAAATCAAGGATTAACA
TATTGTTATGGGTTTTATCAAGAATGGAATCCGAAAATTACAACGCTTCCAGGACTTTAT
TTAATTACAATTTTCTTACCACGATGTTCTGTATTTACACTTCGAATGATCTCATTTGTA
TGTTCTATCATCAATTTTTATTTCATCATTGAGATACGATCAAATATTGAAAAGAAGAAA
TCAGATGATTATGATATACTGTTGAATTCTCTATCAATCGTTTTATTGCCACCTATGTAT
TTTTTCTCATTAATATATTATACGGACATACCAGCAATCACAACTATCCTCAGTACAATA
TACTTTTCAATGAAGAGTCAGCATGTTCTTTCATCGATTTGTGGCTTTCTAAGTGTATTA
ATGCGTCAAACTAACATTTGTTGGGTTGCTGGAATTCTTGGAATGAATATAATTGATGCA
ATGATTGCAAAAGTCTATCCTAATATTGATTTGAAAAAATCTTCAACTAAACACCTGTAT
GTGGCAATCAAAAATCATTTGAAGAAACCAAAAATGTTATATGAACTTATTTTGAAAATT
CTCATAAATTTTTATGGCTATATAATTGTCATTCTCTGTTTTGTACTGTTCCTTATTTTA
AATGGCTCAATTGTTGTTGGAGACAAACAAGCACATGTCGCAACGATACATTTACCACAA
CTTTTTTATTTTTCGCTTTTTACTCTTGTATTTGGTACATCTATTTGGTTGCCAAATTTA
AAAAAAAATTTAATCCATTTAAGAAATATAAAATGTTTATTAATCATATTTTTTACAATG
ATTATAATGGTCGTTATTATACAACAAAATACTATCGTTCATCCATATCTTTTAGCAGAT
AATCGTCATTATACTTTCTATATATGGAACAGATTTTATGGTAGATATTGGTTTTTTAAA
TATACAATGATTCCGGTCTATATATTTGGATTATATAATGTATATGCATCATTAAGCGGT
TCTATTGGATTTAAAATTTTCTATACGTTTTCTATTTTACTAACATTTTGTCTTCAAACA
CTCATAGAAGTTCGATATTTTTTGATACCATTTTTAATTTTAAGACTTTTCAAAAACAAC
CATGAGAAAAAATGGTCATTCATGGAACTGATAATAAATATTTTAATCAATTTTCTGACG
TTCAAAATATTTTTCTCAATTAAAATAAACTGGCCAGAGTTTCAAGAACCTCAACGTATC
ATATGGTGA
>g6585.t1 Gene=g6585 Length=442
MHSSILIGFYSIITLILFDKVYQTSQSIIDELFHINQGLTYCYGFYQEWNPKITTLPGLY
LITIFLPRCSVFTLRMISFVCSIINFYFIIEIRSNIEKKKSDDYDILLNSLSIVLLPPMY
FFSLIYYTDIPAITTILSTIYFSMKSQHVLSSICGFLSVLMRQTNICWVAGILGMNIIDA
MIAKVYPNIDLKKSSTKHLYVAIKNHLKKPKMLYELILKILINFYGYIIVILCFVLFLIL
NGSIVVGDKQAHVATIHLPQLFYFSLFTLVFGTSIWLPNLKKNLIHLRNIKCLLIIFFTM
IIMVVIIQQNTIVHPYLLADNRHYTFYIWNRFYGRYWFFKYTMIPVYIFGLYNVYASLSG
SIGFKIFYTFSILLTFCLQTLIEVRYFLIPFLILRLFKNNHEKKWSFMELIINILINFLT
FKIFFSIKINWPEFQEPQRIIW
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g6585.t1 | PANTHER | PTHR12989 | ALPHA-1,2-GLUCOSYLTRANSFERASE ALG10 | 6 | 442 | 6.3E-104 |
| 32 | g6585.t1 | PIRSF | PIRSF028810 | Alg10 | 1 | 442 | 1.1E-118 |
| 1 | g6585.t1 | Pfam | PF04922 | DIE2/ALG10 family | 26 | 396 | 4.2E-108 |
| 22 | g6585.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 58 | - |
| 31 | g6585.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 59 | 86 | - |
| 16 | g6585.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 87 | 105 | - |
| 29 | g6585.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 106 | 128 | - |
| 18 | g6585.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 129 | 147 | - |
| 23 | g6585.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 148 | 174 | - |
| 15 | g6585.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 175 | 215 | - |
| 26 | g6585.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 216 | 240 | - |
| 20 | g6585.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 241 | 259 | - |
| 28 | g6585.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 260 | 280 | - |
| 14 | g6585.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 281 | 291 | - |
| 25 | g6585.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 292 | 317 | - |
| 19 | g6585.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 318 | 336 | - |
| 30 | g6585.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 337 | 355 | - |
| 13 | g6585.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 356 | 366 | - |
| 24 | g6585.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 367 | 393 | - |
| 21 | g6585.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 394 | 404 | - |
| 27 | g6585.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 405 | 425 | - |
| 17 | g6585.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 426 | 442 | - |
| 12 | g6585.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 23 | - |
| 4 | g6585.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 71 | 93 | - |
| 8 | g6585.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 106 | 128 | - |
| 10 | g6585.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 166 | 188 | - |
| 5 | g6585.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 224 | 246 | - |
| 9 | g6585.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 261 | 280 | - |
| 11 | g6585.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 293 | 312 | - |
| 6 | g6585.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 332 | 354 | - |
| 3 | g6585.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 367 | 389 | - |
| 7 | g6585.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 409 | 431 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0106073 | dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity | MF |
| GO:0006488 | dolichol-linked oligosaccharide biosynthetic process | BP |
KEGG
Orthology
- This transcript has been annotated with K03850 (alpha-1,2-glucosyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
