Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g6585 g6585.t8 TTS g6585.t8 17716314 17716314
chr_2 g6585 g6585.t8 isoform g6585.t8 17716396 17717853
chr_2 g6585 g6585.t8 exon g6585.t8.exon1 17716396 17716988
chr_2 g6585 g6585.t8 cds g6585.t8.CDS1 17716396 17716988
chr_2 g6585 g6585.t8 exon g6585.t8.exon2 17717050 17717853
chr_2 g6585 g6585.t8 cds g6585.t8.CDS2 17717050 17717356
chr_2 g6585 g6585.t8 TSS g6585.t8 17717854 17717854

Sequences

>g6585.t8 Gene=g6585 Length=1397
ATGCATTCAAGTATTTTAATTGGATTTTATTCGATAATAACATTGATTTTGTTTGATAAA
GTTTATCAAACATCACAATCAATAATAGACGAATTATTTCATATAAATCAAGGATTAACA
TATTGTTATGGGTTTTATCAAGAAGTAAGTGATGAATCATGATTTTTATTAATAATCAAA
TCTTTATTTAACATCATCAATTTTACTTTAAGTGGAATCCGAAAATTACAACGCTTCCAG
GACTTTATTTAATTACAATTTTCTTACCACGATGTTCTGTATTTACACTTCGAATGATCT
CATTTGTATGTTCTATCATCAATTTTTATTTCATCATTGAGATACGATCAAATATTGAAA
AGAAGAAATCAGATGATTATGATATACTGTTGAATTCTCTATCAATCGTTTTATTGCCAC
CTATGTATTTTTTCTCATTAATATATTATACGGACATACCAGCAATCACAACTATCCTCA
GTACAATATACTTTTCAATGAAGAGTCAGCATGTTCTTTCATCGATTTGTGGCTTTCTAA
GTGTATTAATGCGTCAAACTAACATTTGTTGGGTTGCTGGAATTCTTGGAATGAATATAA
TTGATGCAATGATTGCAAAAGTCTATCCTAATATTGATTTGAAAAAATCTTCAACTAAAC
ACCTGTATGTGGCAATCAAAAATCATTTGAAGAAACCAAAAATGTTATATGAACTTATTT
TGAAAATTCTCATAAATTTTTATGGCTATATAATTGTCATTCTCTGTTTTGTACTGTTCC
TTATTTTAAATGGCTCAATTGTTGTTGGAGACAAACAAGCACATGTCGCAACGATACATT
TACCACAACTTTTTTATTTTTCGCTTTTTACTCTTGTATTTGGTACATCTATTTGGTTGC
CAAATTTAAAAAAAAATTTAATCCATTTAAGAAATATAAAATGTTTATTAATCATATTTT
TTACAATGATTATAATGGTCGTTATTATACAACAAAATACTATCGTTCATCCATATCTTT
TAGCAGATAATCGTCATTATACTTTCTATATATGGAACAGATTTTATGGTAGATATTGGT
TTTTTAAATATACAATGATTCCGGTCTATATATTTGGATTATATAATGTATATGCATCAT
TAAGCGGTTCTATTGGATTTAAAATTTTCTATACGTTTTCTATTTTACTAACATTTTGTC
TTCAAACACTCATAGAAGTTCGATATTTTTTGATACCATTTTTAATTTTAAGACTTTTCA
AAAACAACCATGAGAAAAAATGGTCATTCATGGAACTGATAATAAATATTTTAATCAATT
TTCTGACGTTCAAAATATTTTTCTCAATTAAAATAAACTGGCCAGAGTTTCAAGAACCTC
AACGTATCATATGGTGA

>g6585.t8 Gene=g6585 Length=299
MKSQHVLSSICGFLSVLMRQTNICWVAGILGMNIIDAMIAKVYPNIDLKKSSTKHLYVAI
KNHLKKPKMLYELILKILINFYGYIIVILCFVLFLILNGSIVVGDKQAHVATIHLPQLFY
FSLFTLVFGTSIWLPNLKKNLIHLRNIKCLLIIFFTMIIMVVIIQQNTIVHPYLLADNRH
YTFYIWNRFYGRYWFFKYTMIPVYIFGLYNVYASLSGSIGFKIFYTFSILLTFCLQTLIE
VRYFLIPFLILRLFKNNHEKKWSFMELIINILINFLTFKIFFSIKINWPEFQEPQRIIW

Protein features from InterProScan

Transcript Database ID Name Start End E.value
2 g6585.t8 PANTHER PTHR12989 ALPHA-1,2-GLUCOSYLTRANSFERASE ALG10 2 299 1.9E-68
1 g6585.t8 Pfam PF04922 DIE2/ALG10 family 2 253 3.7E-72
11 g6585.t8 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 72 -
17 g6585.t8 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 73 97 -
13 g6585.t8 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 98 116 -
20 g6585.t8 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 117 137 -
10 g6585.t8 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 138 148 -
18 g6585.t8 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 149 174 -
15 g6585.t8 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 175 193 -
21 g6585.t8 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 194 212 -
12 g6585.t8 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 213 223 -
16 g6585.t8 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 224 250 -
14 g6585.t8 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 251 261 -
19 g6585.t8 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 262 282 -
9 g6585.t8 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 283 299 -
3 g6585.t8 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 81 103 -
8 g6585.t8 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 118 137 -
5 g6585.t8 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 150 169 -
4 g6585.t8 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 189 211 -
6 g6585.t8 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 224 246 -
7 g6585.t8 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 266 288 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0106073 dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity MF
GO:0006488 dolichol-linked oligosaccharide biosynthetic process BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values