Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g6585 g6585.t9 TTS g6585.t9 17716314 17716314
chr_2 g6585 g6585.t9 isoform g6585.t9 17716396 17717853
chr_2 g6585 g6585.t9 exon g6585.t9.exon1 17716396 17717641
chr_2 g6585 g6585.t9 cds g6585.t9.CDS1 17717039 17717641
chr_2 g6585 g6585.t9 exon g6585.t9.exon2 17717710 17717853
chr_2 g6585 g6585.t9 cds g6585.t9.CDS2 17717710 17717853
chr_2 g6585 g6585.t9 TSS g6585.t9 17717854 17717854

Sequences

>g6585.t9 Gene=g6585 Length=1390
ATGCATTCAAGTATTTTAATTGGATTTTATTCGATAATAACATTGATTTTGTTTGATAAA
GTTTATCAAACATCACAATCAATAATAGACGAATTATTTCATATAAATCAAGGATTAACA
TATTGTTATGGGTTTTATCAAGAATGGAATCCGAAAATTACAACGCTTCCAGGACTTTAT
TTAATTACAATTTTCTTACCACGATGTTCTGTATTTACACTTCGAATGATCTCATTTGTA
TGTTCTATCATCAATTTTTATTTCATCATTGAGATACGATCAAATATTGAAAAGAAGAAA
TCAGATGATTATGATATACTGTTGAATTCTCTATCAATCGTTTTATTGCCACCTATGTAT
TTTTTCTCATTAATATATTATACGGACATACCAGCAATCACAACTATCCTCAGTACAATA
TACTTTTCAATGAAGAGTCAGCATGTTCTTTCATCGATTTGTGGCTTTCTAAGTGTATTA
ATGCGTCAAACTAACATTTGTTGGGTTGCTGGAATTCTTGGAATGAATATAATTGATGCA
ATGATTGCAAAAGTCTATCCTAATATTGATTTGAAAAAATCTTCAACTAAACACCTGTAT
GTGGCAATCAAAAATCATTTGAAGAAACCAAAAATGTTATATGAACTTATTTTGAAAATT
CTCATAAATTTTTATGGCTATATAATTGTCATTCTCTGTTTTGTACTGTTCCTTATTTTA
AATGGCTCAATTGTTGGTAAGTTTTAATCGAAGTCTCTTTTGAAACATCATAATTCTATA
CTTTTTATTCTTTTTAGTTGGAGACAAACAAGCACATGTCGCAACGATACATTTACCACA
ACTTTTTTATTTTTCGCTTTTTACTCTTGTATTTGGTACATCTATTTGGTTGCCAAATTT
AAAAAAAAATTTAATCCATTTAAGAAATATAAAATGTTTATTAATCATATTTTTTACAAT
GATTATAATGGTCGTTATTATACAACAAAATACTATCGTTCATCCATATCTTTTAGCAGA
TAATCGTCATTATACTTTCTATATATGGAACAGATTTTATGGTAGATATTGGTTTTTTAA
ATATACAATGATTCCGGTCTATATATTTGGATTATATAATGTATATGCATCATTAAGCGG
TTCTATTGGATTTAAAATTTTCTATACGTTTTCTATTTTACTAACATTTTGTCTTCAAAC
ACTCATAGAAGTTCGATATTTTTTGATACCATTTTTAATTTTAAGACTTTTCAAAAACAA
CCATGAGAAAAAATGGTCATTCATGGAACTGATAATAAATATTTTAATCAATTTTCTGAC
GTTCAAAATATTTTTCTCAATTAAAATAAACTGGCCAGAGTTTCAAGAACCTCAACGTAT
CATATGGTGA

>g6585.t9 Gene=g6585 Length=248
MHSSILIGFYSIITLILFDKVYQTSQSIIDELFHINQGLTYCYGFYQEWNPKITTLPGLY
LITIFLPRCSVFTLRMISFVCSIINFYFIIEIRSNIEKKKSDDYDILLNSLSIVLLPPMY
FFSLIYYTDIPAITTILSTIYFSMKSQHVLSSICGFLSVLMRQTNICWVAGILGMNIIDA
MIAKVYPNIDLKKSSTKHLYVAIKNHLKKPKMLYELILKILINFYGYIIVILCFVLFLIL
NGSIVGKF

Protein features from InterProScan

Transcript Database ID Name Start End E.value
2 g6585.t9 PANTHER PTHR12989 ALPHA-1,2-GLUCOSYLTRANSFERASE ALG10 6 245 5.2E-48
3 g6585.t9 PANTHER PTHR12989:SF10 DOL-P-GLC:GLC(2)MAN(9)GLCNAC(2)-PP-DOL ALPHA-1,2-GLUCOSYLTRANSFERASE-RELATED 6 245 5.2E-48
1 g6585.t9 Pfam PF04922 DIE2/ALG10 family 26 245 1.9E-54
15 g6585.t9 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 58 -
20 g6585.t9 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 59 86 -
10 g6585.t9 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 87 105 -
17 g6585.t9 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 106 127 -
13 g6585.t9 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 128 138 -
16 g6585.t9 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 139 159 -
12 g6585.t9 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 160 165 -
19 g6585.t9 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 166 186 -
14 g6585.t9 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 187 215 -
18 g6585.t9 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 216 240 -
11 g6585.t9 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 241 248 -
9 g6585.t9 SignalP_EUK SignalP-noTM SignalP-noTM 1 23 -
8 g6585.t9 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 71 93 -
7 g6585.t9 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 106 128 -
5 g6585.t9 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 138 160 -
4 g6585.t9 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 167 189 -
6 g6585.t9 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 220 242 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0106073 dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity MF
GO:0006488 dolichol-linked oligosaccharide biosynthetic process BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values